RESEARCH OF AMYLOLYTIC ACTIVITY OF CULTURAL LIQUIDS IN BIOTECHNOLOGY OF SYMBIOTIC CROPS МЕDUSOMYCES GISEVII AND ORYZAMYCES INDICI

2021 ◽  
pp. 64-78
Author(s):  
N. Masalitina ◽  
O. Bliznjuk ◽  
A. Belinska ◽  
O. Varankina ◽  
L. Krichkovska
Keyword(s):  
Culture Medium ◽  
Sucrose Concentration ◽  
Culture Liquid ◽  
Amylolytic Activity ◽  
Raw Material ◽  
Biomass Growth ◽  
Material Source ◽  
Microbial Symbiont ◽  
Tea Fungus ◽  
Growing Conditions

Based on the literature review found that the natural symbionts Medusomyces gisevii and Oryzamyces indici biotechnology are a valuable objects. It is urgent to find ways to impact on it in order to obtain these or other products of its life activity. At present, it is urgent to search for microorganisms producing enzymes, including amylase. One of the most promising in terms of biological objects is a natural microbial symbiont Medusomyces Gisevii (tea fungus) and Oryzamyces Indici, which, thanks to the not identical, microbiological composition and different growing conditions may have a different composition of metabolites. Studies of the amylolytic activity of the culture liquid Medusomyces Gisevii and Oryzamyces indici with different cultivation periods have been carried out. Cultivation of the fungus was carried out in the laboratory according to the classic method. The optimal concentration of sucrose for Medusomyces Gisevii and Oryzamyces Indici biomass growth was set at 5%. Sucrose concentration of 15% and above is not recommended for use due to inhibition of biomass growth with increasing concentration of carbohydrates in the culture medium. It is established that these symbionts start to show the amylolytic activity already on the 10th day of cultivation cultivation in standard nutrient medium, medium supplemented with 10 % milk and serum-based medium and stores it in the course of the experiment (30 days). Over time the amylolytic activity increases. However, the intensity of metabolism of the microorganisms, the criterion of which is the ratio of the total and exogenous amylase is most pronounced in the early stages of cultivation. It was found that the culture medium of polycultures shows high amylolytic activity. This fact allows us to consider the Medusomyces Gisevii and Oryzamyces Indici inoculum as a promising biotechnological raw material source of amylase enzyme.  

Determination of main raw material source in bar soaps using mid-infrared spectroscopy combined with classification tools

2021 ◽  
Vol 164 ◽  
pp. 106029
Author(s):  
Diego Maciel Gerônimo ◽  
Sheila Catarina de Oliveira ◽  
Frederico Luis Felipe Soares ◽  
Patricio Peralta-Zamora ◽  
Noemi Nagata
Keyword(s):  
Infrared Spectroscopy ◽  
Raw Material ◽  
Material Source ◽  
Mid Infrared ◽  
Mid Infrared Spectroscopy

scholarly journals Investigating microcrystalline cellulose crystallinity using Raman spectroscopy

Cellulose ◽  
2021 ◽  
Author(s):  
Ana Luiza P. Queiroz ◽  
Brian M. Kerins ◽  
Jayprakash Yadav ◽  
Fatma Farag ◽  
Waleed Faisal ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Microcrystalline Cellulose ◽  
Web Application ◽  
Principal Component ◽  
Raw Material ◽  
Least Squares Regression ◽  
Analysis Model ◽  
General Reference ◽  
Material Source ◽  
Univariate Model

AbstractMicrocrystalline cellulose (MCC) is a semi-crystalline material with inherent variable crystallinity due to raw material source and variable manufacturing conditions. MCC crystallinity variability can result in downstream process variability. The aim of this study was to develop models to determine MCC crystallinity index (%CI) from Raman spectra of 30 commercial batches using Raman probes with spot sizes of 100 µm (MR probe) and 6 mm (PhAT probe). A principal component analysis model separated Raman spectra of the same samples captured using the different probes. The %CI was determined using a previously reported univariate model based on the ratio of the peaks at 380 and 1096 cm−1. The univariate model was adjusted for each probe. The %CI was also predicted from spectral data from each probe using partial least squares regression models (where Raman spectra and univariate %CI were the dependent and independent variables, respectively). Both models showed adequate predictive power. For these models a general reference amorphous spectrum was proposed for each instrument. The development of the PLS model substantially reduced the analysis time as it eliminates the need for spectral deconvolution. A web application containing all the models was developed. Graphic abstract

scholarly journals Evaluation of Usefulness of the Designed Laboratory Photobioreactor for Microalgae Cultivation in Controlled Conditions

2016 ◽  
Vol 20 (1) ◽  
pp. 13-22
Author(s):  
Beata Brzychczyk ◽  
Zbigniew Kowalczyk ◽  
Jan Giełżecki
Keyword(s):  
Culture Medium ◽  
Dry Mass ◽  
Biomass Growth ◽  
Microalgae Cultivation ◽  
Freshwater Microalgae ◽  
Laboratory Conditions ◽  
Medium Concentration ◽  
Controlled Conditions ◽  
The Impact

AbstractThe objective of the paper was to analyse the use of the designed photobioreactor for freshwater microalgae cultivation in the controlled laboratory conditions. The work covered the design and construction of photobioreactors (PBR) and setting up comparative cultivations of freshwater microalgae chlorelli vulgaris along with determination of the biomass growth intensity for a varied amount of supplied culture medium. It was found out that the constructed PBR may be used for microalgae cultivation in the controlled conditions. The impact of the culture medium amount on the growth of chlorelli vulgaris was proved. As a result of the increase of culture medium concentration to 30.1-120.4 ml·l−1 of water, dry mass in photobioreactorsincreased respectively from 1.33 g·dm−3 to 4.68 g·dm−3.

scholarly journals Black shale ore of Big Karatau is a raw material source of rare and rare earth elements

2021 ◽  
pp. 105733
Author(s):  
B.K. Kenzhaliyev ◽  
T. Yu Surkovа ◽  
M.N. Azlan ◽  
S.B. Yulusov ◽  
B.M. Sukurov ◽  
...  
Keyword(s):  
Rare Earth ◽  
Rare Earth Elements ◽  
Black Shale ◽  
Raw Material ◽  
Material Source

Copernicus data to boost raw material source management: Illustrations from the RawMatCop programme

2021 ◽  
Vol 74 ◽  
pp. 102384
Author(s):  
Sara Kasmaeeyazdi ◽  
Mehdi Abdolmaleki ◽  
Elsy Ibrahim ◽  
Jingyi Jiang ◽  
Ignacio Marzan ◽  
...  
Keyword(s):  
Raw Material ◽  
Material Source ◽  
Source Management

scholarly journals Greenhouse Tomato Producers’ Views on Soilless Agriculture in Antalya

2018 ◽  
Vol 6 (4) ◽  
pp. 460
Author(s):  
Bilge Gözener ◽  
Halime Dereli
Keyword(s):  
Food Industry ◽  
Agricultural Land ◽  
Raw Material ◽  
Vegetable Production ◽  
Material Source ◽  
Greenhouse Production ◽  
Tomato Production ◽  
Greenhouse Tomato ◽  
Tomato Paste ◽  
Greenhouse Vegetable

Tomato comes as the most commonly produced, consumed and subject for trading in the world. Alongside fresh consumption, on the other hand it forms the most significant raw material source of food industry, especially for tomato paste, frozen and dried vegetable-fruit and canned food industry. Turkey's greenhouse vegetable production field for 2016 year is 675173 decars and Antalya forms 51% of this field. Tomato forms 61.72% of Antalya's greenhouse production. The main material of the research consists of interviews made with producers resided in 5 villages/towns, where greenhouse tomato production is carried out densely in Antalya city, Alanya district. In 48 villages and towns, greenhouse tomato production is carried out, according to the official records. In the chosen areas, 365 producers exist. 20% of these producers (73) form the sample size. In the research, it was determined that the producers' average agricultural land possession is 9.13 decars and in 40.53% of these areas they grew tomatoes. None of these producers are engaged in contractual growing. All of the yield is produced for the edible (as table-top item). After the harvest, all of the products are sold in the wholesales market in county and city. 7.89% of the producers have no information on soilless agriculture, as 10.52% of them think that it has no advantages and 73.36% of them recommend traditional agriculture.

scholarly journals AMINO ACID COMPOSITION OF LEAVES OF THREE SPECIES OF ARTEMISIA L. GROWING IN THE ELTON REGION

2021 ◽  
pp. 219-225
Author(s):  
Галина Табаленкова
Keyword(s):  
Amino Acids ◽  
Czech Republic ◽  
Amino Acid ◽  
Dry Weight ◽  
Biologically Active ◽  
Raw Material ◽  
Buffer System ◽  
Amino Acid Analyzer ◽  
Growing Conditions ◽  
High Level

The results of studies of the qualitative composition and quantitative content of amino acids (AAs) in the leaves of three plant species of the genus Artemisia, widespread in the Elton region, were presented. Protein AAs were determined on an AAA T-339 amino acid analyzer (Czech Republic) after hydrolysis of a sample in 6N HCl at 105 °C for 24 h, free AAs – on an AAA-400 amino acid analyzer (Czech Republic) in a lithium buffer system. The protein AAs amount varied from 66 mg / g in A. lerchiana to 113 mg / g dry weight in A. santonica. 17 AAs were found in composition of these species, aspartic and glutamic acids were dominant. The content of free AAs varied from 4.4 mg / g in A. santonica to 8.3 mg / g dry weight in A. pauciflora. 14 AAs have been identified, among them proline was the predominant free AA. The share of proline was 75-81% of the total free AAs. Among the minor components, 3-4 compounds with a content above 2% dominated. The free AAs contain 3 non-proteinogenic ones (ornithine, citruline, and γ-aminobutyric acid). A. lerchiana and A. pauciflora species were similar in protein and free amino acids, probably due to the same growing conditions. A high level of free proline, together with a complex of biologically active substances in Artemisia species, which grow abundantly in the Elton region, allow to consider the possibility of their use as a medicinal raw material.

The antioxidant activity of phenolic compounds isolated from the bark of Ryukyu pine trees (Pinus luchuensis Mayr.)

2021 ◽  
Vol 17 ◽  
Author(s):  
Hanagasaki Takashi ◽  
Teruya Shoei
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Radical Scavenging ◽  
Raw Material ◽  
Tree Bark ◽  
Cosmetic Products ◽  
Material Source ◽  
Pine Tree ◽  
Pine Trees ◽  
Pinus Luchuensis

Background: Considering that many foreign tourists visit Okinawa, Japan, to purchase some cosmetic products, there is an urgent need to create cosmetic products native to Okinawa. As the Ryukyu pine tree, which is endemic to Okinawa, has been used as a source of wood, investigating the possible use of its bark is recommended. Using this natural resource from Okinawa would aid in promoting the products of Okinawa’s unique brands. As a result, this study was designed to isolate useful materials for cosmetic production. Therefore, fractionation was conducted based on a few types of chromatographies, after which the extracted product of the Ryukyu pine tree (Pinus luchuensis Mayr.) bark was analyzed, and its polyphenol contents compared. Methods: Bark of the Ryukyu pine tree cultivated in the northern mountainous region of the Okinawa Main Island was used for ASE extraction using ultrapure water at 130°C. DIAION HP20 with methanol and two HPLC fractionation types were subsequently used for phenolic compound isolation. Results: ASE extraction and HP20 and HPLC fractionations resulted in an isolation of several compounds: threo-1,2-bis-(4-hydroxy-3-methoxyphenyl)-propane-1,3-diol (compound 1; 0.03% w/w of an ASE extract), erythro-1,2-bis-(4-hydroxy-3-methoxyphenyl)-propane-1,3-diol (compound 2; 0.03% w/w of an ASE extract), catechin (0.11% w/w of an ASE extract), and vanillin (0.31% w/w of an ASE extract). In addition, the value of its antioxidant activity by the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging capacity assay was 3.3 mmol-trolox eq./g, 2.6 mmol-trolox eq./g, 9.7 mmol-trolox eq./g and 0.7 mmol-trolox eq./g for compound 1, compound 2, catechin, and vanillic acid, respectively. Conclusion: These phenolic compounds possess whitening and anti-aging potentials. Therefore, Ryukyu pine tree bark would be a useful raw material source for cosmetic production.

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