Investigation of the Influence of Gamma Radiation on Structural Transformations in Portlandcement Stone

The radiation resistance of concrete under the influence of large doses of gamma radiation was investigated. To study the behavior of concrete under the influence of gamma radiation, two series of samples were taken: оne was the reference sample, and the other was exposed to gamma radiation. The temperature of the irradiated samples during testing did not exceed 40 °C, the reference temperature was also accepted to be 40 °C. The dose of gamma radiation was 109 rad. Its value corresponds to the dose that concrete can receive when it comes into contact with high-level radioactive waste from the Shelter over 300 years. Characteristics of an industrial gamma radiation equipment are: radiation energy is 1.25 MeV and dose rate is 2 Mrad/h. The use of such equipment allows reaching a dose of 109 rad in less than a month, and 108 rad - in 4-5 days. Concretes that were 28 days old and stored under normal conditions were exposed to gamma radiation.

Exosomal MicroRNA-151a-3p Improves the Sensitivity to Radiotherapy via the Interaction between p53 and Histone Deacetylase 5

Background: Tumor-derived exosomal microRNAs are key elements of the cell-cell communications response to lots of stimuli. However, various functions of the exosome in tumor suppression by radiotherapy (RT) are not clearly understood. Our study showed a previously unknown interaction of p53 and histone deacetylase 5 (HDAC5) by radiation exposure in hepatocellular carcinoma (HCC). Methods: Using serial ultracentrifugation methods, radiation and non-radiation exosomes were purified to investigate the radioresistance of miRNA151a-3p. Radiation doses were treated in 2gy and 4gy using radiation equipment X-RAD 320 to observe the expression of HDAC5 and p53 in hepatic cancer cells. Exosomal miRNA bioinformatics analysis was conducted to find a variation in the miRNA configuration inside Exosome after radiation exposure.Results: HDAC5 and p53 interacted by exposure to radiation, which increased exosome release and altered microRNAs' composition within exosomes. Also, we have described the intercommunication occurring between irradiated and untreated cells via exosomal microRNAs that affect tumor proliferation. In particular, the expression of exosomal microR151a-3p was markedly reduced by radiation treatment. We confirmed that inhibition of exosomal microR151a-3p promotes suppression of non-irradiated cancer cells, thereby increasing RT sensitivity. Conclusion: our present findings demonstrated HDAC5 is a key component of the p53-mediated release of exosomes resulting in tumor suppression through exosomal microRNA-151a-3p in response to radiation. Finally, we highlight the important role of exosomal microRNA 151a-3p as a biomarker in enhancing RT sensitivity.

Abstract 14656: Fusion of Fluoroscopy and Echocardiography During the Mitraclip® Procedure Reduces Time to Transseptal Puncture and Radiation Exposure

Introduction: Transseptal puncture (TSP) is an essential step in percutaneous structural heart interventions, such as the MitraClip® procedure. Radiation exposure is a hazard for Interventional Cardiologists, Echocardiographers, and patients. Advancements in shielding and radiation equipment have reduced this exposure, but further reduction is desired. EchoNav® (Philips) fuses fluoroscopy and echocardiography resulting in a single multimodal image display. Prior studies demonstrated reduced time to TSP with use of EchoNav® but failed to show significant reduction in radiation. We hypothesized that increased experience using EchoNav® would further decrease TSP time and radiation dose. Methods: Single center, retrospective analysis evaluated 202 patients undergoing MitraClip® procedure pre and post-EchoNav® from 6/2010 to 12/2019: 8 pre and 194 post. We measured time to TSP and radiation exposure by Air Kerma and dose area product (DAP). For the post-EchoNav® cases, we evaluated these variables by 2-year time periods to examine change over time. Results: Comparing pre and post-EchoNav ® procedures, time to TSP was non-significantly reduced overall (40.00±14.95 to 33.63±15.92, p=0.23). However, post-EchoNav ® had a decreasing trend (48.00±28.39, 43.47±15.86, 28.45±11.94, 27.55±10.64; R 2 =0.89, p =0.009) that was significantly less than pre-EchoNav ® by the third time period (40.00±14.95 vs 28.45±11.94, p=0.05) and remained less in the fourth (40.00±14.95 vs 27.55±10.64, p=0.03). Radiation was reduced post-EchoNav ® by both DAP (264.52±150.03 to 109.00±97.68) (p=0.02) and Air Kerma (1472.92±883.50 to 582.49±485.28) (p=0.05). Significant radiation reduction occurred by the third time period for Air Kerma (1472.92±883.50 vs 494.12±413,79, p=0.03) and second time period for DAP (264.52±150.03 to 127.51±110.35, p=0.03). Conclusion: These results suggest that use of EchoNav® has a learning curve, but ultimately reduces time to TSP and radiation.

Availability of radiation diagnostic equipment for examination of cancer patients in healthcare institutions

Objectives to analyze the equipment of health care institutions, engaged in providing medical care to patients with cancer, with medical devices for diagnostic radiology. Materials and methods.The data from official reporting forms of medical organizations in Saint Petersburg on the availability of medical equipment for diagnostic radiology were analyzed for the period of 5 years using a continuous sampling method. The obtained data were statistically processed with the calculation of statistical series, extensive and intensive indicators. The Student t-test was used to assess the statistically significant differences in indicators in individual years of observation. Results.In the period from 2013 to 2018, the analysis showed an increase of the availability of diagnostic radiology devices in the medical organizations in St. Petersburg, particularly for ultrasound equipment by 39.7%, x-ray machines by 9.5%, CT machines by 23.9%, MRI machines by 16.6%, SPECT and PET devices. At the same time, a uniform increase in the number of ultrasound and x-ray medical equipment was observed in urban and Federal medical organizations, while the rate of "heavy" radiation medical equipment (CT, MRI, PET, SPECT) was higher in Federal medical institutions. Conclusion.Despite the marked increase in the equipment of medical institutions, a significant part of medical radiation equipment has been in use for 10 years or longer (from 14.4% to 29.8% for certain types of medical equipment) and requires a scheduled replacement.

Nutritional Intervention during Chemical, Biological, Radiological, Nuclear Environments A Dietary Perspective

The future war scenario is based on use and applications of various conventional and non-convectional agents which includes weaponised or non-weaponised chemical, biological, radiological, nuclear (CBRN), toxic industrial materials, direct energy devices/ weapons, and or high yield explosives. These include nerve agents, blood agents, vesicants or skin blistering agents, lung irritants, asphyxiants or choking agents. Biological weapons are basically disease causing microorganisms and other replicating entities including viruses, infectious nucleic acids and prions. These agents have ability to infect host and are highly virulent, pathogenic and dangerous in nature. The interface between ammunitions and above agents is soldier whose physical and mental health is affected as enough precautionary measures are not adopted. The reducing environment thus created has various agents which enter into exposed body and lead to mild to serious damage to various vital parts of the human body. As food is important component for survival and intrinsic to basic human nutrition and health, therefore, it is imperative to develop certain kind of a wholesome meal system which can be consumed by the soldiers tasked with combating CBRN situations during such operations. Such meals can be in the form of solid or liquid type and packaged in suitable delivery system, compatible and amenable with the CBRN suit. Food can be contaminated during CBRN conditions by coming in direct or indirect contact with CBRN agents. Therefore, the food materials to be used under such conditions need to be protected in suitable coverings as consumption of contaminated food can be lethal. Designer meal for CBRN environment is not only suitable for soldiers but also to all human interface dealing with similar scenario viz. the low intensity conflicts and surgical operations, nuclear submarines, cosmonauts, pilots, individuals handling radiation equipment and patient undergoing chemotherapy for cancer.

Research on development of multi - channel analyzer used for monitoring and warning radiation equipment

The subject assigned to this paper presents research on development of multi-channel analyzer used for monitoring and warning environmental radiation equipment under the project KC.05.16/11-15 "Research on manufacturing equipment monitoring and warning radiation". In this thematic we have two subjects that need to be resolved such as: i) Designing spectroscopy amplifier block (AMP) duty pulse signals obtained about few hundred millivolts output from scintillation detector preamplifier, amplified as a few volts and the standard Gaussian pulses shaped to connect to the analog-to-digital converter. The spectroscopy amplifier block can change the gain by digital control to respond to the problem of automatic spectrum stability for multi-channel analyzer systems. ii) Designing analog-to-digital converter block (ADC) in accordance with the actual conditions, such as high stability, fast convertion time, high throughput, and it consums low energy. Selecting suitable microprocessor for fast connection ability, to operate reliably paired with the analog-to-digital converter into a multi-channel analyzer (MCA) serving analysis.