The German Job Search Panel

This report introduces the German Job Search Panel, a longitudinal survey that follows people who register as job seeking over the course of up to two years. The focus of the survey is on job seekers’ well-being and health. An innovative survey app is used to allow for frequent measurement every month and for conducting the experience sampling method. The collected data may be linked to administrative records of the Federal Employment Agency, provided that people give their consent. A subsample of surveyed job seekers took part in hair sampling to measure their cortisol levels. In this report, we describe the sampling procedure, adjustments over the recruitment period and the collected data. We moreover examine selective participation in the panel. It turns out that high-skilled workers, young individuals and women were more likely to sign up. Age increases the probability to take part in the hair sampling. People working in East Germany were more likely to consent to the linkage of survey data and administrative records.

DETERMINATION OF TOXIC, ESSENTIAL AND RELATIVELY ESSENTIAL ELEMENTS LEVEL IN ALPACA HAIR WITH USING OF DIFFERENT SAMPLING TYPES

The method of inductive coupled plasma mass spectrometry (ICP-MS) was used for elemental analysis of alpaca hair. Various methods impact of hair sampling for analysis was evaluated with using deionized water and acetone. Sodium content decrease in the in hair samples washed with deionized water and Mg, Li, V, Ag, Cd, Ba level decrease in samples treated with acetone were found. The results can be explained by sodium attendance in the hair in the form of simple cations that are not bound to any organic compounds by strong bonds, while the results obtained for Mg, Li, V, Ag, Cd, Ba, suggest that the ions of these elements are connected with structural hair formations. Significant differences in the content of other macro- and microelements in alpaca hair treated with deionized water and acetone were not found.

Steroid hormones in hair reveal sexual maturity and competition in wild house mice (Mus musculus domesticus)

Endocrine data from wild populations provide important insight into social systems. However, obtaining samples for traditional methods involves capture and restraint of animals, and/or pain, which can influence the animal’s stress level, and thereby undesirable release of hormones. Here, we measured corticosterone, testosterone and progesterone in the hair of 482 wild-derived house mice that experienced sexual competition while living under semi-natural conditions. We tested whether sex, age, weight and indicators of sexual maturity, reproduction and social conflicts predict hormone concentrations measured in hair (sampling at endpoint). We show that body weight, sex and age significantly predict cumulative testosterone and progesterone levels, allowing the differentiation between subadults and adults in both sexes. Corticosterone was only slightly elevated in older males compared to older females and increased with the level of visible injuries or scars. Testosterone in males positively correlated with body weight, age, testes size, and sperm number. Progesterone in females significantly increased with age, body weight, and the number of embryos implanted throughout life, but not with the number of litters when controlled for age and weight. Our results highlight the biological validity of hair steroid measurements and provide important insight into reproductive competition in wild house mice.

Are we overestimating the utility of hair glucocorticoids? A systematic review exploring the empirical evidence supporting hair glucocorticoids as a measure of stress

Quantitating glucocorticoids (GCs) in hairs is a popular method for assessing chronic stress in studies of humans and animals alike. The cause-and-effect relationship between stress and elevated GC levels in hairs, sampled weeks later, is however hard to prove. This systematic review evaluated the evidence supporting hair glucocorticoids (hGCs) as a biomarker of stress.Only a relatively small number of controlled studies employing hGC analyses have been published, and the quality of the evidence is compromised by unchecked sources of bias. Subjects exposed to stress mostly demonstrate elevated levels of hGCs, and these concentrations correlate significantly with GC concentrations in serum, saliva and feces. This supports hGCs as a biomarker of stress, but the dataset provided no evidence that hGCs are a marker of historical stress. Only in cases where the stressor persisted at the time of hair sampling could a clear link between stress and hGCs be established.

Accurate Estimation of Drug Intake Day by Microsegmental Analysis of a Strand of Hair by Use of Internal Temporal Markers

Background Segmental hair analysis can be useful for estimating the time of drug intake. However, this estimation is currently only accurate to within several months. We previously conducted microsegmental analysis of a strand of hair to visualize drug distribution at a spatial resolution of 0.4 mm, which corresponds to daily hair-growth length. Herein, we describe a procedure for accurately estimating the day of drug intake by using internal temporal markers (ITMs) to mark a timescale in the analyzed strand of hair. Methods Five drugs were administered in a single dose to the subjects, and then administration was stopped for several weeks. Two subsequent cycles of drug administration and similar withdrawal were performed. For analysis, a strand of hair was plucked from the subject's scalp. The first intake day was considered as the unknown and the drugs administered second and third were regarded as the ITMs. The first intake day was estimated based on the distance from hair root end to 3 drug peaks and 3 known days (hair sampling and 2 ITM cycles). Results The drug concentration–hair segment curve had 3 peaks, which reflected the 3 drug cycles. The use of ITMs reduced the error of the true intake day to within 2 days, because the growth rate of the analyzed strand of hair was accounted for by the 2 ITMs. Conclusions The estimated accuracy showed little dependency on drug and individual variation. This procedure for estimating the time of drug intake down to a particular day can be used in drug-related crimes, drug abuse and compliance, and for medical diagnosis.