Types of Solid Lipids on Physical Stability of Resveratrol-Loaded Nanostructured Lipid Carriers

2020 ◽  
Vol 859 ◽  
pp. 203-207 ◽  
Author(s):  
Benchawan Chamsai ◽  
Wipada Samprasit ◽  
Praneet Opanasopit ◽  
Pontip Benjasirimongkol ◽  
Pornsak Sriamornsak
Keyword(s):  
Zeta Potential ◽  
High Stability ◽  
Negative Charge ◽  
Physical Stability ◽  
Nanostructured Lipid Carriers ◽  
Stability Studies ◽  
Sorbitan Monooleate ◽  
Glyceryl Monostearate ◽  
High Shear Homogenization ◽  
The Stability

Resveratrol (Res) loaded nanostructured lipid carriers (NLCs) were prepared by high shear homogenization and ultrasonication technique. Caprylic/capric glyceride (IM) was used as a liquid lipid, while glyceryl monostearate (GMS), beeswax (BW), palmitic acid (PA) and myristic acid (MA) at various concentrations (5, 10, 15, and 20 %w/w) were solid lipids. Polyoxyethylene 20 sorbitan monooleate or polysorbate 80 (Tween® 80) was use as a surfactant. The results showed that all concentration of PA and MA can be prepared the smaller particle NLC than that using GMS and BW according to the small molecule of MA and PA. The zeta potential of Res loaded NLCs were negative charge (-24.30 to -37.80 mV), which could be considered as a stable system. The stability studies revealed that NLCs with MA and PA showed a - high stability in particle size and zeta potential after storage at 40 ± 2°C /75 %RH for 3 months. These results suggested the MA and PA were suitable solid lipids for the NLCs preparation which have potential to be a nanoparticulate carrier for Res delivery.

Optimization of Diclofenac Sodium-Loaded Nanostructured Lipid Carriers (NLCs) Using the Box-Behnken Design

2021 ◽  
Vol 901 ◽  
pp. 137-143
Author(s):  
Pakorn Kraisit ◽  
Narong Sarisuta
Keyword(s):  
Oleic Acid ◽  
Negative Charge ◽  
Diclofenac Sodium ◽  
Nanostructured Lipid Carriers ◽  
Polysorbate 80 ◽  
Particle Sizes ◽  
Glyceryl Monostearate ◽  
Ultrasonic Probe ◽  
Box Behnken Design ◽  
Experimental Values

This study aimed to prepare diclofenac sodium (DCF)–loaded nanostructured lipid carriers (NLCs) (DCF-loaded NLCs) for optimizing the NLCs by using the Box-Behnken design. A hot emulsification method using an ultrasonic probe was employed to prepare DCF-loaded NLCs. The active ingredient, solid lipid, oil, and emulsifier were DCF, glyceryl monostearate (GMS) (X1), oleic acid (X2), and polysorbate 80 (X3), respectively. The DCF-loaded NLCs had particle sizes of 69.29–187.3 nm. The polydispersity index (PDI) was in the range of 0.216–0.516, indicating a relatively narrow size distribution. The zeta potential of all formulations revealed the negative charge and ranged between -26.0 and -42.13 mV. The percentage encapsulation efficiency (%EE) was 92.71%–104.21%. The responses of all model formulations were created and the optimized formulation was selected by Design-Expert® software. The optimal formulation was composed of 2 g GMS, 0.926 g oleic acid, and 2.724 g polysorbate 80. The particle size and PDI experimental values with the optimal formulation did not differ from those predicted and were within the 95% CI. Therefore, the Box-Behnken design could be efficient in formulating and optimizing DCF-loaded NLCs.

Effect of Solid Lipid and Liquid Oil Ratios on Properties of Nanostructured Lipid Carriers for Oral Curcumin Delivery

2014 ◽  
Vol 1060 ◽  
pp. 62-65 ◽  
Author(s):  
Yaowaporn Sangsen ◽  
Punsupang Laochai ◽  
Pravara Chotsathidchai ◽  
Ruedeekorn Wiwattanapatapee
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Sustained Release ◽  
Physicochemical Properties ◽  
Nanostructured Lipid Carriers ◽  
Loading Capacity ◽  
High Shear ◽  
Solid Lipid ◽  
High Shear Homogenization ◽  
Xrd Techniques

In this study, three nanostructured lipid carriers (NLC) formulations comprised of varying ratios of lipid (Compritol® 888 ATO) and oil (Labrafac® CC) including 4:1, 3:2, and 2.5:2.5, were developed by high shear homogenization technique. The effect of different ratios on the physicochemical properties and release profiles of the formulations were investigated. Increasing the amount of liquid oil increased the particle size and zeta potential whereas decreased size distribution of the blank and curcumin loaded NLC. However, the entrapment efficacy and loading capacity of the NLC for curcumin were not increased following such ratios. The different ratios were not influence on the sequence of sustained release of curcumin from the NLC over 60 h. Moreover, the amorphous curcumin and crystalline behavior of the optimized NLC were characterized by DSC and XRD techniques. Thus, the effect of the proportions of solid lipid and liquid oil in the formulations should be considered for development of suitable NLC system for oral curcumin delivery.

scholarly journals FORMULATION AND OPTIMIZATION OF NIFEDIPINE LOADED NANOCARRIERS

2021 ◽  
pp. 311-317
Author(s):  
ASHWINI JADHAV ◽  
BINOY VARGHESE CHERIYAN
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Physical Stability ◽  
Entrapment Efficiency ◽  
Tween 80 ◽  
Physicochemical Characteristics ◽  
Good Choice ◽  
The Stability

Objective: The main aim of this study to formulate a nifedipine-loaded nanocarrier for improving solubility and bioavailability. Methods: To improve the solubility of drug, nifedipine-loaded nanocarrier (lipotomes) were prepared by using the film lipid hydration technique. lipotomes were prepared by using tween 80, which is used for increasing solubility and cetyl alcohol for lipophilic environment. Drug excipients interaction determined by FTIR. lipotomes were characterized for particle size, Entrapment efficiency and zeta potential. lipotomes were optimized by using Design-Expert 12 software. Optimized formula further lyophilized by using different cyroproyectant to improve the stability and oral administration of the drug. Results: FTIR shows there was no interaction between formulation ingredients. Mean particle size, entrapment efficiency, zeta potential was determined and found to be 308.1 nm, 96.7%, 20.1mV, respectively. Surface morphology of lipotomes was observed by a scanning electron microscope (SEM). Optimized lipotomes was lyophilized with Mannitol (8% w/v) was the ideal cryoprotectant to retain the physicochemical characteristics of the OLT formulation after lyophilization. Conclusion: Nifedipine loaded nanocarrier was successfully prepared, using film hydration method. Which have good particle size, EE% and zeta potential. After lyophilization no significant changes was observed in particle size with good physical stability, so it could be a good choice for conventional drug delivery system by doing further investigation as in vitro and in vivo study

scholarly journals SYNTHESIS AND STABILITY TEST OF RESVERATROL-CONJUGATED GOLD NANOPARTICLE WITH POLYVINYL ALCOHOL STABILIZATION

2020 ◽  
pp. 221-229
Author(s):  
SUTRIYO SUTRIYO ◽  
RADITYA ISWANDANA ◽  
MARINA DWI HAFSHARI
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Physical Stability ◽  
Drug Efficacy ◽  
Particle Size Analysis ◽  
Size Analysis ◽  
Chloroauric Acid ◽  
Stability Tests ◽  
The Stability ◽  
Effective Site

Objective: Gold nanoparticles (AuNPs) have been developed as a promising effective site-specific drug to increase drug efficacy and reduce potentialside effects. However, AuNPs are unstable because they easily aggregate. This study aims to produce stable resveratrol (RSV)-conjugated AuNPs usingpolyvinyl alcohol (PVA).Methods: AuNPs were synthesized using the Turkevich method, which involves the reduction of chloroauric acid with sodium citrate as a reductor.AuNPs were then modified with PVA as a stabilizing agent and conjugated with RSV as a drug model in the carrier system. The formed conjugates werecharacterized using ultraviolet–visible spectrophotometry, Fourier transform infrared spectroscopy, particle size analysis, and high-performanceliquid chromatography. Furthermore, stability tests were performed in various media (2% bovine serum albumin [BSA], 1% cysteine, phosphatebufferedsaline [PBS] pH 4, PBS pH 7.4, and 0.9% NaCl) for 28 days.Results: RSV–AuNPs–PVA had a particle size of 78.75 nm, with polydispersity index (PDI) of 0.356, zeta potential of −36.1 mV, and highest entrapmentefficiency of 78.1%±0.7. RSV–AuNPs without PVA stabilization had a particle size of 51.97 nm, with PDI of 0.694 and zeta potential of −24.6 mV. Theresults of the stability tests demonstrated that RSV–AuNPs–PVA was stable in 2% BSA, PBS pH 7.4, PBS pH 4, and NaCl 0.9% and were unstable in 1%cysteine. RSV–AuNPs without PVA were stable in 2% BSA and PBS pH 7.4 and unstable in 1% cysteine, PBS pH 4, and 0.9% NaCl.Conclusion: PVA can improve the physical stability of RSV-AuNPs conjugates.

scholarly journals Influence of process parameters on properties of Nanostructured Lipid Carriers (NLC) formulation.

1970 ◽  
Vol 60 (4) ◽  
Author(s):  
Elwira Lasoń ◽  
Elżbieta Sikora ◽  
Jan Ogonowski
Keyword(s):  
Zeta Potential ◽  
Electrophoretic Mobility ◽  
Process Parameters ◽  
Tween 80 ◽  
Controlled Drug Release ◽  
Sodium Cholate ◽  
Nanostructured Lipid Carriers ◽  
Probe Type ◽  
Influence Of Process Parameters ◽  
The Stability

Nanostructured lipid carriers (NLC) are stable colloidal formulations with notable advantages for drug delivery systems. Thanks to their physicochemical stability, biocompatibility, biodegradability and controlled drug release, they have received increasing attention for the last several years. The aim of the study was to prepare and characterize nanostructured lipid carriers (NLC). Both, the effect of the process parameters and the effect of the preemulsion composition on the NLC properties were investigated. In the work, different type of surfactants (i.e. decyl glucoside, Poloxamer188, Tween 80, sodium cholate) and their combinations were used to stabilize NLC dispersions. Moreover, several kinds of solid lipids (modified beeswax, gliceryl behenate, cetyl palmitate and berry wax) and liquid lipids (caprilic/capric triglyceride and decyl oleate) were applied. An ultrasonication method using a probe type sonicator was used to obtain NLC, and the time and energy of the process were modified throuhout. The physicochemical properties of the formulations, such as particle size, size distribution, polidispersity index were studied using the dynamic light scattering (DLS) method. The electrophoretic mobility of obtained particles was also measured, using the Zetasizer Nano ZS Malvern Instrument based on the Laser Doppler Velocimetry (LDV) technique. Knowing the value of electrophoretic mobility of particles for given conditions, the zeta potential was determined. The obtained results showed that the process parameters and the composition of the preemulsion had significant impact on the nanoparticles structure. The optimal formulations size ranged between 60 and 80 nm, and the value of their zeta potential was up to -30mV. The stability of these systems was further confirmed by macroscopic observation.

scholarly journals Microencapsulation Preservation of the Stability and Efficacy of Citrus Grandis Oil-Based Repellent Formulation against Aedes aegypti during Storage

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3599
Author(s):  
Norashiqin Misni ◽  
Zurainee Mohamed Nor ◽  
Rohani Ahmad ◽  
Nur Raihana Ithnin ◽  
Ngah Zasmy Unyah
Keyword(s):  
Relative Humidity ◽  
Essential Oil ◽  
Zeta Potential ◽  
Aedes Aegypti ◽  
Essential Oils ◽  
Ph Value ◽  
Physical Stability ◽  
Storage Conditions ◽  
Mosquito Repellent ◽  
The Stability

Essential oils have been widely used as an active ingredient in mosquito repellent products. However, essential oils are highly unstable and prone to degradation when exposed to the environment during storage. Microencapsulation techniques help to maintain the stability of molecules in essential oils that are sensitive to environmental stress, and therefore improve shelf life. In this study, the physical stability and efficacy of a repellent formulation consisting of encapsulated Citrus grandis essential oil (CGEO) were evaluated under different storage conditions over a 12-month period by comparing the formulation with a non-encapsulated formulation. The formulations were both stored under two different storage conditions, i.e., 25 ± 2 °C/60% ± 5% relative humidity (RH) and 40 ± 2 °C/75% RH ± 5%, for 12 months. Droplet size, zeta potential, and pH value were measured after 1, 6, and 12 months of storage to determine their stability. For the study of efficacy, each formulation was tested against Aedes aegypti under laboratory conditions. We found that the microencapsulated formulation’s physical characteristics showed insignificant changes as compared with the non-encapsulated formulation during storage. The microencapsulated formulation demonstrated better repellent effects, sustaining high protection (>80%) for 4 more hours of exposure after 12 months of storage as compared with the non-encapsulated formulation that demonstrated high protection for only an hour post application. Microencapsulation helped to preserve the stability of the formulation, which resulted in high protection being maintained for over 12 months of storage.

Design of Nanoemulsions through Combination of Fixed-Volatile Oils

2011 ◽  
Vol 486 ◽  
pp. 123-126 ◽  
Author(s):  
Sirikarn Pengon ◽  
Chutima Limmatvapirat ◽  
Sontaya Limmatvapirat
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Soybean Oil ◽  
High Surface ◽  
Physical Stability ◽  
Visual Observation ◽  
Volatile Oils ◽  
Potential Measurement ◽  
Tea Tree ◽  
The Stability

The aim of study was to comparatively evaluate emulsions containing volatile oils e.g. peppermint oil, optamint and tea tree oil, and those containing the combination of volatile oils with soybean oil. The emulsions were prepared and then characterized by monitoring of particle size, visual observation of creaming and cracking, and zeta potential measurement. The results showed that particle size of emulsions containing only volatile oils was in the micron range (3-10 mm). However, the particle size was decreased to less than 300 nm after incorporation with soybean oil (1:1 by weight), suggesting the formation of nanoemulsions. The nanoemulsions containing both volatile oils and soybean oil demonstrated almost 100% creaming and did not show any evidence of cracking. Additionally, percent creaming and particle size were not significantly changed even after accelerated stability testing. The results indicated the good physical stability. The stabilization of emulsion might relate with the high surface charge as observed by higher zeta potential of emulsions prepared from volatile oils combined with soybeans oil. Glycerides from soybean should give more negative charge to the oleaginous phase that help preventing aggregation of oil droplets and thus increased the stability of nanoemulsions. In conclusion, the results from this study could provide the basic guideline for preparation of stable nanoemulsions for food, cosmetic and pharmaceutical fields in the near future.

An Experimental Investigation on Thermal Conductivity and Viscosity of Graphene doped CNTs /TiO2 Nanofluid

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
A.M. Zetty Akhtar ◽  
M.M. Rahman ◽  
K. Kadirgama ◽  
M.A. Maleque
Keyword(s):  
Thermal Conductivity ◽  
Zeta Potential ◽  
Base Fluid ◽  
Minimum Quantity Lubrication ◽  
Cutting Fluid ◽  
Cutting Zone ◽  
Observation Method ◽  
The Stability ◽  
The Relationship ◽  
Zeta Potential Analysis

This paper presents the findings of the stability, thermal conductivity and viscosity of CNTs (doped with 10 wt% graphene)- TiO2 hybrid nanofluids under various concentrations. While the usage of cutting fluid in machining operation is necessary for removing the heat generated at the cutting zone, the excessive use of it could lead to environmental and health issue to the operators. Therefore, the minimum quantity lubrication (MQL) to replace the conventional flooding was introduced. The MQL method minimises the usage of cutting fluid as a step to achieve a cleaner environment and sustainable machining. However, the low thermal conductivity of the base fluid in the MQL system caused the insufficient removal of heat generated in the cutting zone. Addition of nanoparticles to the base fluid was then introduced to enhance the performance of cutting fluids. The ethylene glycol used as the base fluid, titanium dioxide (TiO2) and carbon nanotubes (CNTs) nanoparticle mixed to produce nanofluids with concentrations of 0.02 to 0.1 wt.% with an interval of 0.02 wt%. The mixing ratio of TiO2: CNTs was 90:10 and ratio of SDBS (surfactant): CNTs was 10:1. The stability of nanofluid checked using observation method and zeta potential analysis. The thermal conductivity and viscosity of suspension were measured at a temperature range between 30˚C to 70˚C (with increment of 10˚C) to determine the relationship between concentration and temperature on nanofluid’s thermal physical properties. Based on the results obtained, zeta potential value for nanofluid range from -50 to -70 mV indicates a good stability of the suspension. Thermal conductivity of nanofluid increases as an increase of temperature and enhancement ratio is within the range of 1.51 to 4.53 compared to the base fluid. Meanwhile, the viscosity of nanofluid shows decrements with an increase of the temperature remarks significant advantage in pumping power. The developed nanofluid in this study found to be stable with enhanced thermal conductivity and decrease in viscosity, which at once make it possible to be use as nanolubricant in machining operation.

Photostability Study on Character and Antioxidant Activity of Tomato Extract (Solanum lycopersicum l.) in Nanostructured Lipid Carrier (NLC) and Conventional Creame

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Noorma Rosita ◽  
Dewi Haryadi ◽  
Tristiana Erawati ◽  
Rossa Nanda ◽  
Widji Soeratri
Keyword(s):  
Antioxidant Activity ◽  
Physical Stability ◽  
Nanostructured Lipid Carrier ◽  
Uvb Radiation ◽  
K Value ◽  
Antioxidant Power ◽  
Solanum Lycopersicum L ◽  
Tomato Extract ◽  
Dpph Method ◽  
High Shear Homogenization

The aim of this study was to investigate the ability of NLC in increasing photostability of tomato extract in term of antioxidant activity. Photostability testing on antioxidant activity of samples were conducted by accelerating method using UVB radiation 32.400 joule for 21 hours radiation. Antioxidant activity was measured by DPPH method. NLC was made by High Shear Homogenization (HPH) method at 24000 rpm for 4 cycles, while conventional creame was made by low speed at 400 rpm. The product were characterized include: pH, viscosity, and particle size. There were had difference characters and physical stability. NLC had smaller size, more homogenous and more stable than conventional creame. It was known that stability of antioxidant activity of tomato extract in NLC system higher than in conventional creame. That was showed with k value, as constanta of rate scavenging activity decreasing in antioxidant power between time (Sigma 2-tail less than 0.005) of NLC and conventional creame were: 2.03x10-2 %/hour ±0.08 (3.94) and 4.71x 10-2 %/ hour ±0.23 (4.88) respectively.

Stability of radical anions derived from substituted 5-nitrofurans investigated by ESR spectroscopy

1985 ◽  
Vol 50 (7) ◽  
pp. 1594-1601 ◽  
Author(s):  
Jiří Klíma ◽  
Larisa Baumane ◽  
Janis Stradinš ◽  
Jiří Volke ◽  
Romualds Gavars
Keyword(s):  
Radical Anion ◽  
High Stability ◽  
Esr Spectroscopy ◽  
Reaction Path ◽  
Order Reaction ◽  
Second Order ◽  
Radical Anions ◽  
Second Order Reaction ◽  
Unpaired Electron Density ◽  
The Stability

It has been found that the decay in dimethylformamide and dimethylformamide-water mixtures of radical anions in five of the investigated 5-nitrofurans is governed by a second-order reaction. Only the decay of the radical anion generated from 5-nitro-2-furfural III may be described by an equation including parallel first- and second-order reactions; this behaviour is evidently caused by the relatively high stability of the corresponding dianion, this being an intermediate in the reaction path. The presence of a larger conjugated system in the substituent in position 2 results in a decrease of the unpaired electron density in the nitro group and, consequently, an increase in the stability of the corresponding radical anions.

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