This study aims to describe the results of characterization of physical properties and design comparation of knee implant. the characterization of the material was intended to determine the morphology using SEM, crystal structure using XRD, and chemical composition using XRF. In both designs, the simulation was carried out to get the total deformation value. Simulation is carried out by loading humans walking, jumping, and downstairs in 0-1.1 seconds. While the comparation was focused on comparizing the total deformation value from the human activity of walking, jumping, and downstairs to determine the optimum design. The finding of this study were SEM showed that many parallel strokes on Ti6Al4V, then XRD test showed that the crystallinity peak was at position 40.5189˚ which were indicated by the crystal orientation index [200] reaching 29.35 counts (cts), and Full Width Half Maximum (FWHM) at an angle of 0.288˚ which had an atomic distance along the length of 2.2246 (Å ) with a relative intensity of 100%. And the XRF test showed the highest chemical content of Ti6Al4V was Ti, amounting to 85.12%. This was indicated by the total maximal deformation of the first design 0.23030 micormeter while the second design was 2.109600 micrometer, so the first design was more recommended for implant use. While comparation of total deformation showed that the first design had the lowest maximum average deformation value. The results showed that the first implant design was the optimum design.