Milk-clotting proteases from Pleurotus albidus: an innovative alternative for the production of Minas frescal cheese

Pleurotus albidus, a naturally growing species in the Amazon region, has been considered a promising source of milk-clotting proteases. The production of such enzymes using lignocellulosic residues is a sustainable alternative to replace mammalian rennet. The application of P. albidus milk-clotting proteases in cheese making has not yet been reported in the scientific literature. The aim of this study was to characterize the milk-clotting proteases of P. albidus and use these enzymes in the production of Minas frescal cheese. For the production of coagulating proteases, the mushroom was grown in açaí seeds supplemented with rice bran (10%, w/w). The parameters affecting the production of coagulant, such as inoculum size, fermentation time, initial pH of cultivation medium and age of the inoculum were evaluated. The coagulant extract obtained under optimal production conditions was evaluated for optimal pH and temperature, pH and temperature stability, effect of ions and inhibitors. Significant production of coagulating proteases was obtained under the following conditions: inoculum size (2.5%), fermentation time (10 days), initial pH of the cultivation medium (6), and inoculum age (10 days). The coagulant exhibited significant catalytic activity in pH 5.0 at 55°C, with stability at 45°C and was completely inhibited by iodoacetic acid. The milk-clotting proteases of P. albidus were efficient for making Minas frescal cheese that presented 55.0% of moisture, 20.0% of lipids and 17.20% of protein. Pleurotus albidus is a potential source of milk-clotting proteases that can be applied in dairy industry for production of fresh Minas frescal cheese.

Thermal Degradation of Linalool-Chemotype Cinnamomum osmophloeum Leaf Essential Oil and Its Stabilization by Microencapsulation with β-Cyclodextrin

The thermal degradation of linalool-chemotype Cinnamomum osmophloeum leaf essential oil and the stability effect of microencapsulation of leaf essential oil with β-cyclodextrin were studied. After thermal degradation of linalool-chemotype leaf essential oil, degraded compounds including β-myrcene, cis-ocimene and trans-ocimene, were formed through the dehydroxylation of linalool; and ene cyclization also occurs to linalool and its dehydroxylated products to form the compounds such as limonene, terpinolene and α-terpinene. The optimal microencapsulation conditions of leaf essential oil microcapsules were at a leaf essential oil to the β-cyclodextrin ratio of 15:85 and with a solvent ratio (ethanol to water) of 1:5. The maximum yield of leaf essential oil microencapsulated with β-cyclodextrin was 96.5%. According to results from the accelerated dry-heat aging test, β-cyclodextrin was fairly stable at 105 °C, and microencapsulation with β-cyclodextrin can efficiently slow down the emission of linalool-chemotype C. osmophloeum leaf essential oil.

Study on the Influence of Self-Circulating and Slot Combined Casing Treatment on the Stability of Transonic Compressor

Casing treatment (CT) has proven to be an effective way to enhance stability, and has a very important role in enhancing the stability of the compressor. Researchers have made great achievements and progress in the study of single-type CT structure, but less research on combined-type CT structure. In this paper, the isolated rotor of a high-load axial-flow compressor is taken as the research object, and the numerical simulation method is used to study the enhancing stability mechanism of the combined-type casing treatment (ASCT) by combining the axial slot casing treatment (ASC) and the self-circulating casing treatment (SCT). The study found that the reasonable choice of the ASCT scheme can make the enhancing stability effect of the ASCT higher than that of the single-type CT structure scheme. Through detailed quantitative analysis of the rotor’s internal flow field, it was found that ASC and SCT can suction the airflow downstream of the rotor passage, and then spray it into the main flow from the upstream of the rotor passage, and the blade tip blockage is reduced, the flow capacity of the blade tip passage is improved, and the rotor stability is enhanced by suppressing tip clearance leakage flow. The ASCT has both the spraying effect of the ASC and the SCT, and has the best improvement effect on the flow blockage zone in the rotor passage, and the obtained enhancing stability effect is also best. In addition, the circulation and re-injection of the airflow after CT has aggravated the flow blending loss in the blade tip zone, which has reduced the rotor efficiency. The ASCT has both the characteristics of the effect of the ASC and the SCT on the rotor efficiency, resulting in a large reduction in the rotor efficiency after using the ASCT.

The improvement of transonic compressor performance by the self-circulating casing treatment

The performance curve of the compressor is limited by the surge boundary, so it is of great significance to increase the stable working range of the compressor. The self-circulating casing treatment is an effective way to improve the stable working range of the compressor. In this paper, the study of the influence of the injector position of the self-circulating casing treatment on the transonic axial flow compressor rotor performance is carried out by using the numerical simulation. The influence mechanism of the injector position on the enhancing stability effect of the self-circulating casing treatment is revealed. It is found that the self-circulating casing treatment can reduce the blade tip blockage by restraining the blade tip clearance leakage flow and changing the trajectory of the tip clearance leakage vortex, thus delaying the deterioration of the rotor tip flow field and improving the rotor stability. When the injector position of the self-circulating casing treatment moves from the upstream of the leading edge of the blade tip to the trailing edge of the blade tip, the enhancing stability effect of the self-circulating casing treatment increases first and then decreases. But the high-velocity jet from the injector of the self-circulating casing treatment aggravates the mixing loss of the rotor tip flow field, so that the rotor efficiency slightly decreases after using the self-circulating casing treatment.