The tribological behavior of a steel/glass ball-on-flat contact was studied by synchronizing the friction measurements with an acoustic emission device and a vision system. The results highlight two distinct friction regimes identified with low and high friction values. Their transition is characterized by a modification of acoustic emission signals. In addition, two main damage and wear mechanisms are identified: the creation and propagation of Hertzian cracks visible on the glass surface and the constitution of an interfacial layer of debris. The different accommodation mechanisms, activated successively or simultaneously, are identified for acoustic emission frequencies between 300 and 700 kHz. Eventually, this approach allows a real-time wear mechanisms identification and gives better insights about acoustic emission signals in relation to tribological systems.