Possibilities of cytological diagnostics in gynecology. Human papillomavirus as an interdisciplinary problem

The article presents an analysis of current information on the etiology, pathogenesis, laboratory diagnosis of human papillomavirus. It is shown that at the current stage of development of laboratory diagnostics there is a reliable screening test — cytological examination of smears taken from the ecto- and endocervix. To diagnose HPV, a combination of microscopic (cytological studies) and molecular genetic (PCR) diagnostic methods is used.

Cytological diagnosis of malignant tumors

The importance of cytological diagnostics in the recognition of cancer has been reflected in a number of reports.

Cellular composition research of serous pleural effusion fluids. Conceptual issues of preanalytics

The purpose of this work was to show the effectiveness of the cytological method on a small number of observations, excluding all possible errors of the preanalytical stage. The paper presents several simple and easily reproducible algorithms for the cytological study of serous pleural effusions with small cellular content. On the example of 20 observations of the study of the cellular composition of serous exudates, a direct dependence of the research results on the preanalytical stage is shown. A complete study of effusion fluids in compliance with all stages of preanalytics and the use of modern methods of cytological diagnostics makes it possible to nullify the options for false-negative.

CYTOLOGICAL DIAGNOSTICS OF MERKEL CELL CARCINOMA (PRIMARY NEURO-ENDOCRINE SKIN CANCER) IN THE MATERIAL OF A SUPERFICIAL SHAVE BIOPSY

Merkel cell carcinoma is a rare malignant primary skin tumor with epithelial and neuroendocrine differentiation. In the presented diagnostic case, the possibility of a cytological method in this material is a scarification biopsy.

Cytological diagnostics of subcutaneous dirofilariasis imitating proliferative lesions in dogs

Subcutaneous dirofilariasis is a disease in animals caused by invasions of nematodes, most often of the following species: Dirofilaria repens, D. tenuis, and D. striata transmitted by mosquitoes. Until recently, D. repens was endemic to the Mediterranean countries in Europe, but, in recent years, it has also been increasingly reported in Central and Eastern Europe. Cytological preparations collected by a fine-needle aspiration biopsy from nodular lesions located in the subcutis and skin of dogs were used to diagnose suspected proliferative lesions of a cancerous or inflammatory nature. The microscopic examination of the delivered cytological preparations revealed erythrocytes (very numerous), neutrophils and eosinophils (quite numerous), macrophages (single), and whole and/or damaged fragments of microfilariae of Dirofilaria sp. in various numbers. It should be noted that the described infection of Dirofilaria repens in Poland and other countries of this latitude will be an increasingly common pathology in dogs. Due to the mosquitoes transmitting the microfilariae, it is a zoonosis that is an increasingly frequent and a more serious threat to humans. In the differential diagnosis of various types of skin and subcutis lesions of unknown aetiology, dirofilariasis should be considered.

Mucin producing carcinomas of the breast. Cytological diagnostics

The aim of the study is to estimate potentiality of the cytological diagnostics of different types of mucin producing carcinomas of the breast.Materials and methods. Cytological evidences of the investigation of 35 patients with mucinous mass in cytological specimens were studied. Different types of mucinous carcinomas which had been diagnosed by cytological method were identified retrospectively.Results. “Pure” mucous carcinomas were divided into two types: hypocellular (A) and hypercellular (B) (n = 9 (25.7 %) and 14 (40 %), respectively). Mixed carcinomas (mucous carcinoma with invasive no special type carcinoma) were noticed of 12 women (34.3 %). 24 cell characteristics of mucin producing carcinomas were identified. Some cytological characteristics were estimated due to multiple factor analysis and discriminant analysis.Conclusions. Investigation results indicated that cytological technique is a high-quality diagnostic method. The fraction of correct classification of mucous carcinoma types based on discriminant analysis was 92–99 %.The study protocol was approved by the biomedical ethics committee of The Altai State Medical University, Ministry of Health of Russia.

POSSIBILITIES OF CYTOLOGICAL DIAGNOSTICS OF LACTATED BREAST ADENOMA. CLINICAL REPORT

We describe a case of lactating adenoma in pregnant woman, 32 week of gestation. Typical features of disease presented in this article was revealed by clinical and microscopic methods and it make possible to diagnose the process before operation. The diagnosis was confirmed by histological investigation of surgical material.

CYTOLOGICAL DIAGNOSTICS OF INTRAOPERATIVE PERITONEAL WASHES IN GASTRIC CANCER

Diagnosis of peritoneal microcanceromatosis is the most important task allowing to determine treatment strategy for patients with stomach cancer. Laparoscopy combined with peritoneal flushing and subsequent cytological examination should be performed to detect the peritoneal microcanceromatosis at the preoperative stage. The objective of this work was to improve cytological diagnostics of peritoneal washings using immunocytochemical techniques and the cell block method. The work was carried out on the basis of 276 surgical peritoneal washings in patients with stomach cancer who were on treatment in the department of high-tech surgery of the Moscow Clinical Scientific Centre of the State Budgetary Healthcare Institution named after Loginov A.S. from June 2016 to June 2018. As a result, the optimal panel of monoclonal antibodies (Ber-EP4, CEA, CK20) was chosen, which increased the sensitivity from 52% to 96% and the specificity of cytological diagnosis from 80% to 98%, and the overall accuracy of the method from 67% to 98%.

Cell Block in Cytological Diagnostics: Review of Preparatory Techniques

Objective: The cell block (CB) technique refers to the processing of sediments, blood clots, or grossly visible tissue fragments from cytological specimens into paraffin blocks that can be cut and stained by the same methods used for histopathology. The technique brings additional tissue architectural information. CB can be used for ancillary techniques such as immunocytochemistry and molecular techniques. Study Design: We reviewed the literature on the various preparatory techniques of CBs. Results: There is a wide range of preparatory techniques for CBs and no golden standard for CBs exists: tens of methods are used in various institutions. The majority of the methods are modified in house techniques with a few commercially available kits. The techniques most commonly used are the plasma/thrombin method, the agar method, and commercially available Histogel- and Cellient CB-methods. Dissatisfaction with the cellular yield of the CBs is common. Conclusions: In the CBs, the cytological material is preserved for future use, which is a tremendous advantage in the era of targeted therapy and biobanking. The CB is thus central to the future of cytology: more can be done with less material and with less invasiveness to the patient.