Nigella sativa Oil Preserved Anxiety-Like, Motor and Memory Related Behaviours and Neuronal Integrity in Dichlorvos Induced Acetyl Cholinesterase Inhibitions in Rats

Organophosphates are irreversible cholinesterase (ChE) inhibitors with neurological consequences, and there is not yet an effective antidote. Here, we investigated the effects of Nigella sativa oil (NSO) on the ChE inhibition, neurobehavioural and histopathological changes following dichlorvos (DDVP) ingestions in rats. Thirty-two male Wistar rats were randomised into four groups, receiving 1 ml/kg of normal saline, 8.8 mg/kg of DDVP, 8.8 mg/kg of DDVP and 1 ml/kg of NSO, and 1 ml/kg of NSO only respectively, for 14 consecutive days. Locomotor, anxiety-like behaviours and spatial working memory were assessed on the 14th day, using open field (OF), Y-maze and modified elevated plus maze paradigms. The rats were euthanized on the 15th day and the brains excised; three brains were fixed for histopathology, and the other five prepared for biochemical analysis of acetyl cholinesterase (AChE). DDVP exposure caused significant reductions in frontal, amygdala and cerebella AChE activity, spontaneous alternations, line crossing and rearing frequencies and time in centre square, and caused increase in freezing period, transfer latency and necrotic-like cells. NSO intervention was able to reverse DDVP effects on AChE activities, explorative, locomotor, anxiety and spatial memory behaviours in co-exposed rats. It also preserved the histological integrity of the investigated brain regions. It can be concluded that NSO, may be potent against organophosphates induced neurotoxicity and their neurobehavioural consequences through the modulation of AChE activities.

Nigella sativa oil: A promising prospective antifungal agent in the manufacture of low-salt soft cheese

The current work studied the in-vivo antifungal activity of Nigella sativa oil (NSO) in ultrafiltered low-salt soft cheese as a proposed replacement for the synthetic preservatives which become unacceptable by consumers. Four different concentrations of NSO were examined during the manufacture of the cheese (0.3, 0.5, 1, and 3 % w/w). The effect of NSO supplementation was examined in 3 parallel lines; a ninepoint hedonic scale was used in the sensorial evaluation of soft cheese free of the fungal inoculum, the physicochemical properties of soft cheese were determined during storage as well as anti-fungal effects of different concentrations of NSO on inoculated cheese with different species of fungi: Candida albicans (104 cfu/ml) and Aspergillus parasiticus (102 cfu/ml) before coagulation. The Nigella sativa oil expressed an antifungal activity by using different levels of NSO which significantly reduced and inhibited the growth of the fungal counts (1.4 log cfu/g for Candida albicans and 2.30 log cfu/g for Aspergillus parasiticus) started from 0.5% concentration of NSO on the 14th day of the storage. In addition, it exhibited different physicochemical properties of soft cheese depending on the level of used NSO. However, the Sensory evaluation of cheese samples revealed the acceptance of soft cheese samples with 0.3% and 0.5% of NSO.

PENERAPAN PEMBERIAN MINYAK JINTAN HITAM (NIGELLA SATIVA OIL) UNTUK PENGOBATAN BIANG KERINGAT (MILIARIA) PADA BAYI DI WILAYAH PMB BRIDA KITY DINARUM S.ST

Latar belakang: Perubahan iklim dan suhu dapat menimbulkan biang keringat (miliaria) pada bayi. Biang keringat adalah ruam kecil yang terasa gatal menyebabkan iritasi kulit. Hal ini dapat diatasi dengan tanaman obat yaitu minyak jintan hitam (Nigella sativa oil) yang berfungsi untuk menghentikan inflamasi atau peradangan. Tujuan: Untuk mengetahui pemberian minyak jintan hitam (Nigella sativa oil) sebagai pengobatan biang keringat (miliaria) pada Bayi. Metode: Karya Tulis Ilmiah ini merupakan jenis penelitian studi kasus. Data diperoleh dari wawancara, observasi atau pengamatan dan studi kepustakaan. Instrumen yang digunakan adalah lembar observasi, lembar check list. Hasil: 3 partisipan mengalami kesembuhan (60%), 2 partisipan mengalami perubahan menjadi miliaria profunda (40%). Kesimpulan: Penerapan pemberian minyak jintan hitam (Nigella sativa oil) efektif mengobati biang keringat (Miliaria) pada bayi. Kata kunci: Minyak jintan hitam (Nigella sativa oil), biang keringat (miliaria), bayi

The effect of black cumin oil (Nigella sativa) on the reproduction of male Wistar rats

Nigella sativa oil is antioxidant compound has the effect that serves to prevent cellular damage. The effect of Nigella sativa oil in the motility and total count of spermatozoa wistar rats. Experimental research with the design of post test only control group design. Wistar rats consists of 24 head and divided into 4 groups consist of control and treatment group. The control group was distilled. The first treatment was of Nigella sativa oil everydays as much with dose 150 mg/kgbw, 250 mg/kgbw, and 350 mg/kgbw, for 16 days, . Statistic test for motility and count of sperm wistar rats use the Kruskal-Wallis followed by Mann Whitney test. Avarage value of motility in the control (21.67±9.832) its lower than treatment. In dose 350 mg/kgbw its highest (52.33±13.292) compare in the treatment 250 mg/kgbw (40.67±17.512) and 150 mg/kgbw (30.67±8.165). avarage value of count sperm in the control (130.83±41.877) its lower than treatments. In dose 350 mg/kgbw its highest (199.67±23.480) compare in the treatment 250 mg/kgbw (187.50±74.538) and 150 mg/kgbw (140.83±32.568). Administration of Nigella sativa oil occur to enhancement motility and number of spermatozoa wistar rats.