Agricultural Bio-Stimulant Activity of Fish Protein Hydrolysate from Common Silver Belly (Gerres subfasciatus)

Taking into account of using the fish industry wastes and for expanding the incentive to a few under used fish species, protein hydrolysates from fish proteins are being set up by a few specialists everywhere throughout the world. Fish Protein Hydrolysates (FPH) are breakdown results of enzymatic transformation of fish proteins into smaller units like peptides, which typically contain 2-20 amino acids. Their worth has been expanded by utilizing them as fluid bio compost, feed supplement and bioorganic fertilizer. From the research carried out at our laboratory, Dry fish silver belly with both microbe and enzyme acquired from cultural broth of lactobacillus species, catalyst papain demonstrated positive upgrade of development and advancement of plants tested. In view of the yield of FPH concentrates from both microbial and enzymatic hydrolysis, the pace of seedling life list in Urad dhal, green gram and okra got higher in 0.1-0.5% convergence of dry fish hydrolysate from lactobacillus treated than in the control variation. With respect to the seeds of urad dhal, green gram and okra, the germination rate and seedling energy list from treated seeds surpassed control by 19.11-128.71%. The positive effect of dry fish protein hydrolysate by microbial procedure on germination rate, seedling life record rate in 3 kinds of seeds is the reason for the improvement of protein hydrolysate item for application in crop creation utilizing dry fish squanders. The Seedling grown in 0.5% concentration of fish hydrolysates showed significant increase in seedling vigour index of Urad dhal (13.29 ± 0.12 %), Green gram (14.1 ± 0.17%) and Okra (5.39 ± 0.43 %) was recorded through one way Analysis of Variance (ANOVA). By HPLC determination amino acid results determined that protein hydrolysates process can be stopped on 7th day (643.56ppm) whereas @ 10th day amino acid decreased (326.24ppm). Results suggest that probiotic assumes a superior job in dry fish protein hydrolysates than compounds when contrasted and yield and prudent astute.

Cytoprotective Effects of Fish Protein Hydrolysates against H2O2-Induced Oxidative Stress and Mycotoxins in Caco-2/TC7 Cells

Many studies report the potent antioxidant capacity for fish protein hydrolysates, including radical scavenging activity and inhibition ability on lipid peroxidation (LPO). In this study, the in vitro cytotoxicity of protein hydrolysates from different salmon, mackerel, and herring side streams fractions was evaluated in the concentration range from 1 to 1:32 dilution, using cloned human colon adenocarcinoma cells TC7 (Caco-2/TC7) by MTT and PT assays. The protein hydrolysates’ antioxidant capacity and oxidative stress effects were evaluated by LPO and reactive oxygen species (ROS) generation, respectively. The antioxidant capacity for pure and bioavailable hydrolysate fraction was also evaluated and compared. Additionally, mycotoxin levels were determined in the fish protein hydrolysates, and their cytoprotective effect against T-2 toxin was evaluated. Both hydrolysates and their bioavailable fraction induced similar cell viability rates. The highest cytoprotective effect was obtained for the salmon viscera protein hydrolysate (HSV), which increased the cell viability by 51.2%. ROS accumulation induced by H2O2 and LPO was suppressed by all pure hydrolysates. The cytoprotective effect of hydrolysates was observed against T-2. Moreover, the different fish fraction protein hydrolysates contain variable nutrients and unique bioactive peptide composition showing variable bioactivity, which could be a useful tool in developing dietary supplements with different target functional properties.

Production And Characterization Of Protein Hydrolysate: Effective Utilization Of Trawl Bycatch

Background: Fishing is an industry that provides livelihood to millions who live along the coast and delicious delicacies for the rest. Hence, improving the profitability of the trade has been of utmost importance and has been the research focus of major fishery industries around the globe. Exploring the potential commercialization of wastes produced by the industry is one way to achieve this goal. In this context, the waste may point towards discarded fishes, fish parts, etc. In this research, we are going to study the possibility to efficiently extract Fish Protein Hydrolysate (FPH) which has a huge commercial value from discarded by-catch fishes. Trawling is a fishing method which when employed often results in the capture of a huge quantity and diversity of non-target species due to lack of selectivity of the trawl net. These non-targeted species often termed as by-catch, are often disposed of. These under-valued species are targeted in this work for producing Fish Protein Hydrolysates (FPHs) using commercially available enzymes such as Papain (FPH1), Proteinase K (FPH2), and their functional characteristics such as solubility, foaming and emulsifying properties, oil and water-binding capacity are compared. The experimental design is as shown in the graphical abstract below.Results: Among the two, Fish Protein Hydrolysates (FPHs), prepared FPH1 has comparatively slightly improved functional properties. Conclusion: It offers the potential to be used for various applications in the food industries.