9,10-Anthracenediones an important class among quinonic compounds of natural and synthetic origin with many applications. Amino- and diaminosubstituted 9,10-anthracenediones are one of the main classes among 9,10-anthracenedione derivatives. Primarily, they are the key structures for obtaining various dyes. Besides applying their dye properties, aminoanthracenediones became the objects for searching for new biologically active compounds with antibacterial, antifungal, anticancer, antioxidant, antiviral, immunostimulating, and antiprotozoal activity in medical chemistry. However, amino- and diamino-substituted 9,10-anthracenediones are widely used as analytical reagents, color, and metal indicators. Areas of the practical application of these compounds are constantly expanding. This mini-review dedicates the advantages of amino- and diamino-9,10-anthracenediones derivatives as biologically active compounds and substances with applied application in various fields.