Abstract
Background
Cordyceps militaris is a fungus that parasitizes insects. Compounds from C. militaris are valuable in medicine and functional food. There are many kinds of bacteria in the natural sclerotia of C. militaris. However, the community structure of microorganisms in samples from different places may be different, and the corresponding ecological functions require experimental verification.
Results
We used high-throughput sequencing technology to analyze bacterial 16S rRNA gene sequences in sclerotia of three samples of C. militaris from Liaoning Province, China. Pseudomonas, Pedobacter, Sphingobacterium, and Serratia were the dominant bacterial genera in the sclerotia. Pseudomonas and Pedobacter are heterotrophic, Sphingobacterium decomposes urea, and Serratia reduce nitrate. Two strains of bacteria, N-2 and N-26, were isolated from the sclerotia of the C. militaris, cultured, biochemically characterized, and identified as Stenotrophomonas maltophilia and Pseudomonas baetica, respectively. When these isolated strains were respectively cocultured with C. militaris, the mycelium biomass, mycelium pellet diameter, and cordycepin content decreased.
Conclusions
Bacteria in sclerotia have an important effect on the growth of C. militaris and the production of its metabolites.