Monitoring of Pollution of Salmonella sp. in Raw Milk Using Virulence Gen Marker

Salmonella sp. is an enteropathogenic organism and it can spread through contaminated food which is rich in nutrition such as raw milk. The goal from this research is to find out the suitable pasteurization and time effect on the growth isolates Salmonella sp. that its invA presence is detected. Seventy-five (75) samples were taken in three locations of milk collection from groups of breeders. 10 ml of milk samples were grown in enrichment culture of modified Tryptic Soy Broth (mTSB) at 37oC for 12 hours. Cellular culture was then grown in differential selective medium of Salmonella Shigella Agar (SSA) and Chromocult Coliform Agar (CCA). From 75 milk samples produced 1392 colonies of typical Salmonella sp. pathogens. Then, isolates were selected using Triple Sugar Iron Agar (TSIA) and Urea Broth media, producing 3 suspected isolates of Salmonella sp which was known from colonies color. Results of confirmation using API 20E and 50 CHE produced two isolates of Salmonella spp. with %ID = 99.6% and one isolate of Salmonella typhi with %ID = 72.1%. All of the isolates were then detected for virulence factor using invA marker and all positive isolates have virulence factor. It indicates that raw milk contains polluted pathogenic bacteria.Keywords: raw milk, enteric pathogenic, virulence gen

Preparation and Properties of 3D Chitosan Microtubes

The preparation of 3D chitosan microtubes from polymer solutions in citric and lactic acids by the wet and dry molding methods is described. The mechanism of formation of the insoluble polymeric layer constructing the walls of these microtubes is characterized. The microtubes obtained from chitosan solutions in citric acid are found to have a fragile porous inner layer. For those obtained from chitosan solutions in lactic acid the morphology, elastic-deformation properties, physicomechanical properties, and biocompatibility were assessed. These samples have smooth outer and inner surfaces with no visible defects and high values of elongation at break. The strength of the microtubes obtained by the dry method is much higher than in the case of the wet one. A high adhesion and high proliferative activity of the epithelial-like MA-104 cellular culture on the surface of our microtubular substrates in model in vitro experiments were revealed. Prospects of using chitosan microtubes as vascular prostheses are suggested.

Extraction of alpha-fetoprotein (AFP) with magnetic albuminoid-imprinted poly(ethylene-co-vinyl alcohol) nanoparticles from human hepatocellular carcinoma HepG2 cellular culture medium

The selective adsorption of alpha-fetoprotein using albuminoid MMIP nanoparticles from the human hepatocellular carcinoma HepG2 cellular culture medium.

A Novel Microfluific Design for Cell Culture and Test

Cellular culture is a complex process for cells are grown under certain given conditions, generally not in vivo. In this paper, a novel microfluidic was designed for cellular culture in a long term. Its structure mainly consists of two parts: channels and chambers. In this architecture, two kinds of channels are designed. One is used to load cell into chambers, the other is for the load of culture solution and drugs. Due to diffusion effect, the culture solution and drugs can permeate into chambers through a amount of ostioles. Finite element simulation was utilized to demonstrast the velocity field distribution and concentration field distribution. Comparisons were made to verify the rationality of the design. The simulation results suggest that this novel microfluidic is appropriate suitable in terms of culturing cells.