THE ROLE OF INTERMEDIATE IRRIGANTS IN THE PREVENTION OF THE FORMATION OF ORANGE-BROWN PRECIPITATE, FORMED DUE TO INTERACTION BETWEEN SODIUM HYPOCHLORITE AND CHLORHEXIDINE: A SPECTROPHOTOMETRIC ANALYSIS.

This study aimed to assess the efciency of intermediate irrigants in the prevention of formation of precipitate, formed due to the interaction of sodium hypochlorite and chlorhexidine when used as root canal irrigants.The formation of orange brown precipitate, due to the reaction of 3% sodium hypochlorite and 2% chlorhexidine was assessed using spectrophotometric analysis. Three intermediate irrigants were then assessed for their capabilities to prevent the formation of the precipitate. Group A- 6.25% sodium metabisulphite, Group B- 10% sodium ascorbate and Group C- saline were used at different volumes and the values were recorded by measuring their optical density. An invitro analysis was further carried out on single rooted teeth, to verify the efcacy of intermediate irrigants. The teeth which were subjected to the tests were sectioned and observed under stereomicroscope for the presence of remnants of orange brown precipitate in the root canals. Results: The spectrophotometric analysis showed peak value at 450nm and the precipitate formation was found at 60µl of 2% chlorhexidine and 1 ml of sodium hypochlorite. There was statistical difference in the optical density values between the groups, with saline having highest optical density values followed by 6.25% sodium metabisulphite and 10% sodium ascorbate group. Wherein 10% sodium ascorbate solution prevented the formation of orange brown precipitate than other two group. It was concluded that 10% sodium ascorbate played a vital role in the reduction of formation of parachloro-aniline, when used as an intermediate irrigant. 6.25% Sodium metabisulphite also prevented the precipitate formation, to a lesser extent than sodium ascorbate. However, saline did not eliminate the formation of the precipitate.

Efficacy of EDDY, passive ultrasonic irrigation and manual irrigation on the removal of orange-brown precipitate

Aim: The aim of this study was to compare the different irrigation activation techniques for removing the orange-precipitate formed after irrigation with sodium hypochlorite (NaOCl) and chlorhexidine (CHX). Methodology: In total, 45-single rooted extracted human teeth were prepared with the ProTaper NEXT rotary system. The roots were respectively irrigated with 5% NaOCl and 2% CHX solutions, and an orange-precipitate formed on the canal walls. The teeth were divided longitudinally, and the precipitate was evaluated with a dental operating microscope (DOM) at 16x magnification before the sections were joined. The halves of roots were then combined, and EDDY, passive ultrasonic activation (PUI) and manual irrigation (MI) techniques were applied for 1 min with 5 ml of distilled water to remove the precipitate. The halves were re-evaluated under the DOM and the residual of percentage of precipitate removed was calculated. The data were evaluated statistically by Kruskal-Wallis tests. Results: A residual orange-precipitate was found in all groups. Although the orange precipitate was effectively removed in the EDDY group, there was no statistically significant difference among EDDY, PUI and MI (p> 0.05). Conclusions: None of the techniques used completely removed the orange precipitate from the root canal surfaces. Among the irrigation activation techniques, there was no superior technique for removing the orange-precipitate from the root canals. How to cite this article: İnce Yusufoglu S, Keskin NB. Efficacy of EDDY, passive ultrasonic ırrigation and manual irrigation on the removal of orange-brown precipitate. Int Dent Res 2021;11(1):1-5. https://doi.org/10.5577/intdentres.2021.vol11.no1.1 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Orange-Brown Precipitate: A Threat to the Success of Endodontic Treatment – An In Vitro Study

AIM: The aim of the present study was to evaluate the effect of various intermediate irrigating solutions in the removal of orange-brown precipitate formed due to alternative use of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) root canal irrigants. METHODS: The root canals of 50 mandibular premolars were prepared using ProTaper Universal Rotary instruments (Dentsply Maillefer, Ballaigues, Switzerland) up to size F3. The roots were randomly divided into five experimental groups specified by the intermediate irrigant used; Group 1: No intermediate irrigant used (control), Group 2: Saline, Group 3: 7% maleic acid (MA), Group 4: 4% sodium thiosulfate, and Group 5: 70% isopropyl alcohol (n = 10). After final irrigation with CHX, the specimens were evaluated for the presence/absence of orange-brown precipitate under stereomicroscope. RESULTS: Mean precipitate for all experimental groups was calculated using ANOVA F test which showed orange-brown precipitate (parachloroanaline) in all groups except Group 4 and Group 5 which were highly significant (p < 0.001) at all the levels of root canal. Intergroup pairwise comparison was done using Tukey’s post hoc test. CONCLUSION: About 7% MA is effective in minimizing the formation of orange-brown precipitate when used as intermediate irrigant between NaOCl and CHX. However, the precipitate was not observed with 4% sodium thiosulfate and 70% isopropyl alcohol.

In-vitro evaluation of intermediate irrigants for removal of para-chloroaniline from the tooth during root canal treatment

Introduction: The need for root canal treatment is to eliminate bacteria from the infected canal system and to prevent recontamination. In this case, most common irrigant used in root canal treatment are sodium hypochlorite (NaOCl) and 2% chlorhexidinegluconate (CHX) have been recommended as a less toxic alternative. This treatment of using chemical irrigants for eliminating bacteria leads to a colour formation in the tooth by formation of parachloroaniline (PCA). Our study focuses on the spectrophotometric analysis of PCA formation during the NaOCl and CHX are mixed and thereby using intermediate irrigant solutions to prevention of orange brown precipitate. Spectrophotometer works on the principle of Beer Lambert’s Law in which - max determination of PCA was done using a wavelength scan between 350nm to 550nm. Furthermore, three different intermediate irrigant solutions were tested to analyse the efficient solution system for prevention of the orange brown precipitate. Group A fresh lime juice extract, group B 14.25% EDTA and group C 70% ethanol was used.Group A (lime group) exhibited the least OD value among all the experimental groups followed by group B (14.25% EDTA) and group C(70% Ethanol) and also statistically proved. Spectrophotometric analysis showed freshly prepared lime juice extract was found to be very effective in prevention of PCA precipitate followed by 14.25% EDTA and 70% ethanol. Thus, this study gives and insight about the analysis and novel ways of prevention of PCA during root canal treatment

Study of the Gold Extraction Using Tetra N-Butyl Ammonium Chloride-Chloroform

The existence of the AuCl4- ion in the solution as the function of pH before performing the extraction of gold in the system of tetra N-butylammonium chloride (TBACI) -chloroform has been studied. The experimental data showed that AuCl4- ion was hydrolyzed at pH 5-10 and, an amorf dark-brown precipitate was appeared at pH 11-14. Amount of gold in the solution at pH 14 before extraction was around 70%. Study of the extraction has been carried out by investigating the influence of pH and TBACI concentration on the extraction efficiency. The experimental result indicated that TBACI was very efficient extractant for the extraction of gold from aqueous halide with the efficiency higher than 99%. The extraction of Gold in the TBACI-chloroform was effective at pH 0-4 with minimum concentration of TBACI 10-3 M, and the calculated Kex (extraction constant) was 5.07x10-4.

Hemolytic and Hemoxidative Activities inMycoplasma penetrans

ABSTRACT Mycoplasma penetrans is a newly isolatedMollicute from the urine of patients infected with human immunodeficiency virus that demonstrates the capacity to adhere to and invade human cells. A previous report, based on assays with mouse red blood cells (RBCs), indicated that M. penetrans lacked hemolytic activity. In our studies, we incubated different isolates ofM. penetrans with various RBC species and observed hemolytic zones surrounding individual mycoplasma colonies. AllM. penetrans strains displayed hemolysis after 2 to 3 days of incubation. Hemolytic activity diffused from single colonies, eventually causing complete lysis. Hemolysis was most pronounced with sheep RBCs, followed by horse, chicken, and human cells. Furthermore, hemolytic activity was demonstrable in both intact mycoplasma cell preparations and spent culture supernatant. However, unlike intact mycoplasmas, the hemolytic activity in the supernatant was dependent on the reducing agent, cysteine. In addition to hemolysis, a brown precipitate was closely associated with mycoplasma colonies, suggesting oxidation of hemoglobin. Absorption spectra indicated that hemoglobin was oxidized to methemoglobin, and the addition of catalase demonstrated H2O2-mediated hemoxidation. Other experiments suggested that hemoxidation enhanced total hemolysis, providing the first evidence of both hemolytic and hemoxidative activities in M. penetrans.