Shouting against Silence: André Brink's Voices of Truth

<p>This thesis investigates the notion of 'Truth' upheld by the South African writer André Brink and discusses his deconstruction of the processes of truth-making. I argue that Brink understands fixed narratives, or received 'truth', as constructed to the detriment of alternative narratives, resulting in their subjugation and eventual loss. In response to authoritative discourses, Brink advocates an ongoing and evolving series of challenging narratives which refuse the closure of narrative possibilities. He urges a constant process of un-forgetting and remembering, a contestational activity that undermines the truth-claims of any oppressive group. Three central texts have been chosen as exemplary of Brink's directive to contest fixed truth claims. The first of these, Devil's Valley, offers an opportunity to examine the novelistic (and often postmodernist) blurring of distinctions between binary oppositions such as 'fact' and 'fiction', 'past' and 'present', 'real' and 'unreal'. In undermining the ostensibly dichotomous nature of these pairings, Brink challenges the bases upon which prejudicial systems such as the Apartheid regime rely. In doing so, he reveals the constructions behind both prejudice and hegemonic discourses, and ultimately undermines these foundations. Similarly, Imaginings of Sand provides a means by which to further explore Brink's engagement with prejudice, and most specifically, with the patriarchal oppression of women. I suggest that Brink's female narratives, in which multiplicity and endless possibility are foregrounded, again contest the constraints imposed by a dominant discourse, offering alternative versions. My final textual examination focuses on A Chain of Voices, in which both the polyphonic narration and the thematic content exemplify the concerns discussed previously Brink's usage of various imagery related to oppressive relationships, I claim, provides metaphors for the manner in which binary relationships are co-dependent, rather than dichotomous, undercutting the justifications associated with privileging certain narratives over others. Brink's Truth, I argue, involves an ongoing contestational process of narratorial imagining, a revisionary project central to both the prejudicial environment of Apartheid South Africa, in which much of Brink's work was written, and also to the larger context of prejudice in all its forms and geographical locations.</p>

Shouting against Silence: André Brink's Voices of Truth

<p>This thesis investigates the notion of 'Truth' upheld by the South African writer André Brink and discusses his deconstruction of the processes of truth-making. I argue that Brink understands fixed narratives, or received 'truth', as constructed to the detriment of alternative narratives, resulting in their subjugation and eventual loss. In response to authoritative discourses, Brink advocates an ongoing and evolving series of challenging narratives which refuse the closure of narrative possibilities. He urges a constant process of un-forgetting and remembering, a contestational activity that undermines the truth-claims of any oppressive group. Three central texts have been chosen as exemplary of Brink's directive to contest fixed truth claims. The first of these, Devil's Valley, offers an opportunity to examine the novelistic (and often postmodernist) blurring of distinctions between binary oppositions such as 'fact' and 'fiction', 'past' and 'present', 'real' and 'unreal'. In undermining the ostensibly dichotomous nature of these pairings, Brink challenges the bases upon which prejudicial systems such as the Apartheid regime rely. In doing so, he reveals the constructions behind both prejudice and hegemonic discourses, and ultimately undermines these foundations. Similarly, Imaginings of Sand provides a means by which to further explore Brink's engagement with prejudice, and most specifically, with the patriarchal oppression of women. I suggest that Brink's female narratives, in which multiplicity and endless possibility are foregrounded, again contest the constraints imposed by a dominant discourse, offering alternative versions. My final textual examination focuses on A Chain of Voices, in which both the polyphonic narration and the thematic content exemplify the concerns discussed previously Brink's usage of various imagery related to oppressive relationships, I claim, provides metaphors for the manner in which binary relationships are co-dependent, rather than dichotomous, undercutting the justifications associated with privileging certain narratives over others. Brink's Truth, I argue, involves an ongoing contestational process of narratorial imagining, a revisionary project central to both the prejudicial environment of Apartheid South Africa, in which much of Brink's work was written, and also to the larger context of prejudice in all its forms and geographical locations.</p>

Grid Quality Measures for PEBI Grids

Combining the Perpendicular Bisector (PEBI) grids with the Two Point Flux Approximation (TPFA) scheme demonstrates a potential to accurately model on unstructured grids, conforming to the geological and engineering features of real grids. However, with the increased complexity and resolution of the grids, the PEBI conditions will inevitably be violated in some cells and the approximation properties will be compromised. The objective is to develop accurate and practical grid quality measures that quantify such errors. We critically evaluated the existing grid quality measures and found them lacking predictive power in several areas. The available k-orthogonality measures predict error for flow along the strata, although TPFA provides an accurate approximation. The false-positive results are not only misleading but can overwhelm further analysis. We developed the so-called "truncation error" grid measure which is probably the most accurate measure for flow through a plane face and accurately measures the error along the strata. We also quantified the error due to the face curvature. Curved faces are bound to exist in any real grid. The impact of the quality of the 2-D Delaunay triangulation on TPFA approximation properties is usually not taken into account. We investigate the impact of the size of the smallest angles that can cause considerable increase of the condition number of the matrix and an eventual loss of accuracy, demonstrated with simple examples. Based on the analysis, we provide recommendations. We also show how the size of the largest angles impacts the approximation quality of TPFA. Furthermore, we discuss the impact of the change of the permeability on the TPFA approximation. Finally, we present simple tools that reservoir engineers can use to incorporate the above-mentioned grid quality measures into a workflow. The grid quality measures discussed up to now are static. We also sketch the further extension to dynamic measures, that is, how the static measures can be used to detect change in the flow behavior, potentially leading to increased error. We investigate a comprehensive set of methods, several of them new, to measure the static grid quality of TPFA on PEBI grids and possible extension to dynamic measures. All measures can be easily implemented in production reservoir simulators and examined using the suggested tools in a workflow.

Maturyoshka: a maturase inside a maturase, and other peculiarities of the novel chloroplast genomes of marine euglenophytes

Organellar genomes often carry group II introns, which occasionally encode proteins called maturases that are important for splicing. The number of introns varies substantially among various organellar genomes, and bursts of introns have been observed in multiple eukaryotic lineages, including euglenophytes, with more than 100 introns in their plastid genomes. To examine the evolutionary diversity and history of maturases, an essential gene family among euglenophytes, we searched for their homologs in newly sequenced and published plastid genomes representing all major euglenophytes' lineages. We found that maturase content in plastid genomes has a patchy distribution, with a maximum of eight of them present in Eutreptiella eupharyngea. The most basal lineages of euglenophytes, Eutreptiales, share the highest number of maturases, but the lowest number of introns. We also identified a peculiar convoluted structure of a gene located in an intron, in a gene within an intron, within yet another gene, present in some Eutreptiales. Further investigation of functional domains of identified maturases shown that most of them lost at least one of the functional domains, which implies that the patchy maturase distribution is due to frequent inactivation and eventual loss over time. Finally, we identified the diversified evolutionary origin of analysed maturases, which were acquired along with the green algal plastid or horizontally transferred. These findings indicate that euglenophytes' plastid maturases have experienced a surprisingly dynamic history due to gains from diversified donors, their retention, and loss.

The Global Fund: why anti-corruption, transparency and accountability matter

Background The creation of the Global Fund to Fight AIDS, Tuberculosis and Malaria, also known as the Global Fund, was prompted by the lack of a timely and effective global response, and the need for financing to fight against three devastating diseases: HIV/AIDS, tuberculosis, and malaria. During the formation of the Global Fund, necessary anti-corruption, transparency, and accountability (ACTA) structures were not put in place to prevent fraud and corruption in its grants, which resulted in the misuse of funds by grant recipients and an eventual loss of donor confidence in 2011. The Global Fund has instituted various ACTA mechanisms to address this misuse of funding and the subsequent loss of donor confidence, and this paper seeks to understand these implementations and their impacts over the past decade, in an effort to probe ACTA more deeply. Results By restructuring the governing committees in 2011, and the Audit and Finance; Ethics and Governance; and Strategy Committees in 2016, the Global Fund has delineated committee mandates and strengthened the Board’s oversight of operations. Additionally, the Global Fund has adopted a rigorous risk management framework which it has worked into all aspects of its functioning. An Ethics and Integrity Framework was adopted in 2014 and an Ethics Office was established in 2016, resulting in increased conflict of interest disclosures and greater considerations of ethics within the organization. The Global Fund’s Office of the Inspector General (OIG) has effectively performed internal and external audits and investigations on fraud and corruption, highlighted potential risks for mitigation, and has implemented ACTA initiatives, such as the I Speak Out Now! campaign to encourage whistleblowing and educate on fraud and corruption. Conclusions From 2011 onwards, the Global Fund has developed a number of ACTA mechanisms which, in particular, resulted in reduced grant-related risks and procurement fraud as demonstrated by the decreased classification from high to moderate in 2017, and the reduction of investigations in 2018 respectively. However, it is crucial that the Global Fund continues to evaluate the effectiveness of these mechanisms; monitor for potential perverse impacts; and make necessary changes, when and where they are needed.

Characterization of Sonic Events Present in Natural-Urban Hybrid Habitats Using UMAP and SEDnet: The Case of the Urban Wetlands

We investigated whether the use of technological tools can effectively help in manipulating the increasing volume of audio data available through the use of long field recordings. We also explored whether we can address, by using these recordings and tools, audio data analysis, feature extraction and determine predominant patterns in the data. Similarly, we explored whether we can visualize feature clusters in the data and automatically detect sonic events. Our focus was primarily on enhancing the importance of natural-urban hybrid habitats within cities, which benefit communities in various ways, specifically through the natural soundscapes of these habitats that evoke memories and reinforce a sense of belonging for inhabitants. The loss of sonic heritage can be a precursor to the extinction of biodiversity within these habitats. By quantifying changes in the soundscape of these habitats over long periods of time, we can collect relevant information linked to this eventual loss. In this respect, we developed two approaches. The first was the comparison among habitats that progressively changed from natural to urban. The second was the optimization of the field recordings’ labeling process. This was performed with labels corresponding to the annotations of classes of sonic events and their respective start and end times, including events temporarily superimposed on one another. We compared three habitats over time by using their sonic characteristics collected in field conditions. Comparisons of sonic similarity or dissimilarity among patches were made based on the Jaccard coefficient and uniform manifold approximation and projection (UMAP). Our SEDnet model achieves a F1-score of 0.79 with error rate 0.377 and with the area under PSD-ROC curve of 71.0. In terms of computational efficiency, the model is able to detect sound events from an audio file in a time of 14.49 s. With these results, we confirm the usefulness of the methods used in this work for the process of labeling field recordings.

Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells

Tissue-resident memory (TRM) T cells are emerging as critical components of the immune response to cancer; yet, requirements for their ongoing function and maintenance remain unclear. APCs promote TRM cell differentiation and re-activation but have not been implicated in sustaining TRM cell responses. Here, we identified a novel role for dendritic cells in supporting TRM to melanoma. We showed that CD8 TRM cells remain in close proximity to dendritic cells in the skin. Depletion of CD11c+ cells results in rapid disaggregation and eventual loss of melanoma-specific TRM cells. In addition, we determined that TRM migration and/or persistence requires chemotaxis and adhesion mediated by the CXCR6/CXCL16 axis. The interaction between CXCR6-expressing TRM cells and CXCL16-expressing APCs was found to be critical for sustaining TRM cell–mediated tumor protection. These findings substantially expand our knowledge of APC functions in TRM T-cell homeostasis and longevity.

Bifurcation drives the evolution of assembly-line biosynthesis

Reprogramming biosynthetic assembly-lines is a topic of intense interest. This is unsurprising as the scaffolds of most antibiotics in current clinical use are produced by such pathways. The modular nature of assembly-lines provides a direct relationship between the sequence of enzymatic domains and the chemical structure of the product, but rational reprogramming efforts have been met with limited success. To gain greater insight into the design process, we wanted to examine how Nature creates assembly-lines and searched for biosynthetic pathways that might represent evolutionary transitions. By examining the biosynthesis of the anti-tubercular wollamides, we show how whole gene duplication and neofunctionalization can result in pathway bifurcation. Importantly, we show that neofunctionalization occurs primarily through intragenomic recombination. This pathway bifurcation leads to redundancy, providing the genetic robustness required to enable large structural changes during the evolution of antibiotic structures. Should the new product be none-functional, gene loss can restore the original genotype. However, if the new product confers an advantage, depreciation and eventual loss of the original gene creates a new linear pathway. This provides the blind watchmaker equivalent to the design, build, test cycle of synthetic biology.

Analysis of the Social-Ecological Causes of Deforestation and Forest Degradation in Ghana: Application of the DPSIR Framework

Globally, forests provide several functions and services to support humans’ well-being and the mitigation of greenhouse gases (GHGs). The services that forests provide enable the forest-dependent people and communities to meet their livelihood needs and well-being. Nevertheless, the world’s forests face a twin environmental problem of deforestation and forest degradation (D&FD), resulting in ubiquitous depletion of forest biodiversity and ecosystem services and eventual loss of forest cover. Ghana, like any tropical forest developing country, is not immune to these human-caused D&FD. This paper reviews Ghana’s D&FD driven by a plethora of pressures, despite many forest policies and interventions to ensure sustainable management and forest use. The review is important as Ghana is experiencing an annual D&FD rate of 2%, equivalent to 135,000 hectares loss of forest cover. Although some studies have focused on the causes of D&FD on Ghana’ forests, they failed to show the chain of causal links of drivers that cause D&FD. This review fills the knowledge and practice gap by adopting the Driver-Pressures-State-Impacts-Responses (DPSIR) analytical framework to analyse the literature-based sources of causes D&FD in Ghana. Specifically, the analysis identified agriculture expansion, cocoa farming expansion, illegal logging, illegal mining, population growth and policy failures and lapses as the key drivers of Ghana’s D&FD. The study uses the DPSIR analytical framework to show the chain of causal links that lead to the country’s D&FD and highlights the numerous interventions required to reverse and halt the ubiquitous perpetual trend of D&FD in Ghana. Similar tropical forest countries experiencing D&FD will find the review most useful to curtail the menace.