Supplemental figure for "Odorous Compound Removal Performance and Water Properties of a Biotrickling Filter Installed in a Piggery"

This is a supplemental figure 1 for the manuscript entitled "Odorous Compound Removal Performance and Water Properties of a Biotrickling Filter Installed in a Piggery." This figure S1 shows trends in elimination capacity.

Supplemental figure for "Odorous Compound Removal Performance and Water Properties of a Biotrickling Filter Installed in a Piggery"

This is a supplemental figure 1 for the manuscript entitled "Odorous Compound Removal Performance and Water Properties of a Biotrickling Filter Installed in a Piggery." This figure S1 shows trends in elimination capacity.

Molecular Probes to Evaluate the Synthesis and Production Potential of an Odorous Compound (2-methylisoborneol) in Cyanobacteria

The volatile metabolite, 2-Methylisoborneol (2-MIB) produced by cyanobacterial species, causes odor and taste problems in freshwater systems. However, simple identification of cyanobacteria that produce such off-flavors may be insufficient to establish the causal agent of off-flavor-related problems as the production-related genes are often strain-specific. Here, we designed a set of primers for detecting and quantifying 2-MIB-synthesizing cyanobacteria based on mibC gene sequences (encoding 2-MIB synthesis-catalyzing monoterpene cyclase) from various Oscillatoriales and Synechococcales cyanobacterial strains deposited in GenBank. Cyanobacterial cells and environmental DNA and RNA were collected from both the water column and sediment of a eutrophic stream (the Gong-ji Stream, Chuncheon, South Korea), which has a high 2-MIB concentration. Primer sets mibC196 and mibC300 showed universality to mibC in the Synechococcales and Oscillatoriales strains; the mibC132 primer showed high specificity for Pseudanabaena and Planktothricoides mibC. Our mibC primers showed excellent amplification efficiency (100–102%) and high correlation among related variables (2-MIB concentration with water RNA r = 689, p < 0.01; sediment DNA r = 0.794, p < 0.01; and water DNA r = 0.644, p < 0.05; cyanobacteria cell density with water RNA and DNA r = 0.995, p < 0.01). These primers offer an efficient tool for identifying cyanobacterial strains possessing mibC genes (and thus 2-MIB-producing potential) and for evaluating mibC gene expression as an early warning of massive cyanobacterial occurrence.

Sulphide Biooxidation in Activated Sludge Diffusion Process: Microbiological and Biochemical Effects

Sulphide was adopted as odorous compound in a simulation of AS Diffusion, an interesting process to treat odors at wastewater treatment plants by diffusing odorous air into aerobic basins. Its behaviour were experimentally evaluated along with its effects on the biomass and the biological processes supposed by some author in an AS diffusion test. Two bench scale sequencing batch reactors (SBRs) were fed in parallel on real primary sewage and monitored after adding increasing concentrations of sulphide to one of them. In this reactor, an average sulphide removal of 94% was measured. Microbial biochemical activity and composition did not show relevant variations after the addition of sulphide, and the good features of activated sludge flocs were maintained also in terms of sludge settleability.

Application of electronic nose to effectiveness monitoring of air contaminated with toluene vapors biofiltration process

The research presents the application of electronic nose (combined with MLR model) to on-line effectiveness monitoring of biofiltration of air contaminated with hydrophobic, odorous compound (toluene vapors). The research was conducted using two-section biotrickling filter inhabited by Candida environmental isolates. Gas chromatography was used as the comparative technique to obtain reliable quantification of toluene concentration in the samples. After about 200 hours of the process, a removal efficiency of 49% was obtained.