Abstract: Python is the finest, easily adoptable object-oriented programming language developed by Guido van Rossum, and first released on February 20, 1991 It is a powerful high-level language in the recent software world. In this paper, our discussion will be an introduction to the various Python tools applicable for Machine learning techniques, Data Science and IoT. Then describe the packages that are in demand of Data science and Machine learning communities, for example- Pandas, SciPy, TensorFlow, Theano, Matplotlib, etc. After that, we will move to show the significance of python for building IoT applications. We will share different codes throughout an example. To assistance, the learning experience, execute the following examples contained in this paper interactively using the Jupiter notebooks. Keywords: Machine learning, Real world programming, Data Science, IOT, Tools, Different packages, Languages- Python.