liver response
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2022 ◽  
Author(s):  
Seddik Hammad ◽  
Christoph Ogris ◽  
Amnah Othman ◽  
Pia Erdoesi ◽  
Wolfgang Schmidt-Heck ◽  
...  

The liver has a remarkable capacity to regenerate and thus compensates for repeated injuries through toxic chemicals, drugs, alcohol or malnutrition for decades. However, largely unknown is how and when alterations in the liver occur due to tolerable damaging insults. To that end, we induced repeated liver injuries over ten weeks in a mouse model injecting carbon tetrachloride (CCl4) twice a week. We lost 10% of the study animals within the first six weeks, which was accompanied by a steady deposition of extracellular matrix (ECM) regardless of metabolic activity of the liver. From week six onwards, all mice survived, and in these mice ECM deposition was rather reduced, suggesting ECM remodeling as a liver response contributing to better coping with repeated injuries. The data of time-resolved paired transcriptome and proteome profiling of 18 mice was subjected to multi-level network inference, using Knowledge guided Multi-Omics Network inference (KiMONo), identified multi-level key markers exclusively associated with the injury-tolerant liver response. Interestingly, pathways of cancer and inflammation were lighting up and were validated using independent data sets compiled of 1034 samples from publicly available human cohorts. A yet undescribed link to lipid metabolism in this damage-tolerant phase was identified. Immunostaining revealed an unexpected accumulation of small lipid droplets (microvesicular steatosis) in parallel to a recovery of catabolic processes of the liver to pre-injury levels. Further, mild inflammation was experimentally validated. Taken together, we identified week six as a critical time point to switch the liver response program from an acute response that fosters ECM accumulation to a tolerant 'survival' phase with pronounced deposition of small lipid droplets in hepatocytes potentially protecting against the repetitive injury with toxic chemicals. Our data suggest that microsteatosis formation plus a mild inflammatory state represent biomarkers and probably functional liver requirements to resist chronic damage.


Author(s):  
Mohamed A. Dkhil ◽  
Esam M. Al-Shaebi ◽  
Mona Khalil ◽  
Ahmed S. Alazzouni ◽  
Saleh Al-Quraishy

2020 ◽  
Vol 21 (21) ◽  
pp. 8250
Author(s):  
Venkat R. Pannala ◽  
Shanea K. Estes ◽  
Mohsin Rahim ◽  
Irina Trenary ◽  
Tracy P. O’Brien ◽  
...  

Liver disease and disorders associated with aberrant hepatocyte metabolism can be initiated via drug and environmental toxicant exposures. In this study, we tested the hypothesis that gene and metabolic profiling can reveal commonalities in liver response to different toxicants and provide the capability to identify early signatures of acute liver toxicity. We used Sprague Dawley rats and three classical hepatotoxicants: acetaminophen (2 g/kg), bromobenzene (0.4 g/kg), and carbon tetrachloride (0.3 g/kg), to identify early perturbations in liver metabolism after a single acute exposure dose. We measured changes in liver genes and plasma metabolites at two time points (5 and 10 h) and used genome-scale metabolic models to identify commonalities in liver responses across the three toxicants. We found strong correlations for gene and metabolic profiles between the toxicants, indicative of similarities in the liver response to toxicity. We identified several injury-specific pathways in lipid and amino acid metabolism that changed similarly across the three toxicants. Our findings suggest that several plasma metabolites in lipid and amino acid metabolism are strongly associated with the progression of liver toxicity, and as such, could be targeted and clinically assessed for their potential as early predictors of acute liver toxicity.


Immunity ◽  
2020 ◽  
Vol 53 (3) ◽  
pp. 627-640.e5 ◽  
Author(s):  
Sophie Tran ◽  
Ines Baba ◽  
Lucie Poupel ◽  
Sébastien Dussaud ◽  
Martine Moreau ◽  
...  

Medicine ◽  
2020 ◽  
Vol 99 (31) ◽  
pp. e21211
Author(s):  
Eriseld Krasniqi ◽  
Giacomo Barchiesi ◽  
Marco Mazzotta ◽  
Laura Pizzuti ◽  
Alice Villa ◽  
...  

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Meredith A Sommars ◽  
Krithika Ramachandran ◽  
Madhavi D Senagolage ◽  
Christopher R Futtner ◽  
Derrik M Germain ◽  
...  

Transcription is tightly regulated to maintain energy homeostasis during periods of feeding or fasting, but the molecular factors that control these alternating gene programs are incompletely understood. Here, we find that the B cell lymphoma 6 (BCL6) repressor is enriched in the fed state and converges genome-wide with PPARα to potently suppress the induction of fasting transcription. Deletion of hepatocyte Bcl6 enhances lipid catabolism and ameliorates high-fat-diet-induced steatosis. In Ppara-null mice, hepatocyte Bcl6 ablation restores enhancer activity at PPARα-dependent genes and overcomes defective fasting-induced fatty acid oxidation and lipid accumulation. Together, these findings identify BCL6 as a negative regulator of oxidative metabolism and reveal that alternating recruitment of repressive and activating transcription factors to shared cis-regulatory regions dictates hepatic lipid handling.


2019 ◽  
Author(s):  
Meredith A Sommars ◽  
Krithika Ramachandran ◽  
Madhavi D Senagolage ◽  
Christopher R Futtner ◽  
Derrik M Germain ◽  
...  

2018 ◽  
Vol 19 (12) ◽  
pp. 3875 ◽  
Author(s):  
Stefania Cannito ◽  
Chiara Milani ◽  
Andrea Cappon ◽  
Maurizio Parola ◽  
Mario Strazzabosco ◽  
...  

The cholangipathies are a class of liver diseases that specifically affects the biliary tree. These pathologies may have different etiologies (genetic, autoimmune, viral, or toxic) but all of them are characterized by a stark inflammatory infiltrate, increasing overtime, accompanied by an excess of periportal fibrosis. The cellular types that mount the regenerative/reparative hepatic response to the damage belong to different lineages, including cholagiocytes, mesenchymal and inflammatory cells, which dynamically interact with each other, exchanging different signals acting in autocrine and paracrine fashion. Those messengers may be proinflammatory cytokines and profibrotic chemokines (IL-1, and 6; CXCL1, 10 and 12, or MCP-1), morphogens (Notch, Hedgehog, and WNT/β-catenin signal pathways) and finally growth factors (VEGF, PDGF, and TGFβ, among others). In this review we will focus on the main molecular mechanisms mediating the establishment of a fibroinflammatory liver response that, if perpetuated, can lead not only to organ dysfunction but also to neoplastic transformation. Primary Sclerosing Cholangitis and Congenital Hepatic Fibrosis/Caroli’s disease, two chronic cholangiopathies, known to be prodrome of cholangiocarcinoma, for which several murine models are also available, were also used to further dissect the mechanisms of fibroinflammation leading to tumor development.


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