Comparative Study of Developed Tomato Soup with Market Soup

Streetfood has popular in Indian market and consumed as convenient to eat with reasonable price but sold open dirt areas that found unhygienic, not free from microbial contamination and having low nutritional quality. The present investigation was carried out to develop vegetable tomato soup and compare with street tomato soups and assess their sensory quality and microbial study. Microbial parameters were used to affect the shelf life of the products. The developed tomato soups were acceptable in comparison to Street tomato soups. Hence, low cost nutritious developed tomato soup can be acceptable with good storage stability.

Synthesis of some new 6-aryl-3-(4-isopropylphenyl)[1,2,4]triazolo[3,4-b][1,3,4] thiadiazoles and its anti-microbial study

New series of fused 1,2,4-triazoles, i.e., 3-(4-isopropylphenyl)-6-substituted phenyl-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazoles (5a-5j) have been synthesized via a four-step procedure and using eco-friendly reaction condition at some steps of the synthesis. It was adopted by the formation of hydrazide of methyl 4-isopropylbenzoate (1) followed by reaction with CS2 in basic media to afford potassium salt, which on cyclized to our essential step, 4-amino-5-(4-isopropylphenyl)-4H-1,2,4-triazole-3-thiol (4). The desired adducts (5a-5j) were formed by (4) on reaction with various aromatic acids in POCl3 media. The newly synthesized triazolo- thiadiazoles have been characterized by different spectroscopic techniques and investigated for their in vitro antibacterial and antifungal activity. It was revealed that the compounds 5a, 5c, 5h, and 5i showed interesting antibacterial and antifungal activity compared to the used reference standard.

Synthesis, SAR, Molecular Docking and Anti-Microbial Study of Substituted N-bromoamido-2-aminobenzothiazoles

Background: Benzothiazoles are reported to have bioorganic and pharmaceutical chemistry applications. Introduction: A series of substituted N-bromoamido-2-aminobenzothiazoles was synthesized from substituted anilines via 2-aminobenzothiazoles and it was further evaluated for its antimicrobial activity. Methods: All the newly synthesized compounds were characterized by FT-IR, NMR and mass spectra and purity profiles were studied by HPLC analysis. The antimicrobial testing (MIC determination) was newly performed with agar micro-broth dilution method for these analogs. Results: Among the synthesized compound 3b showed the highest activity with MIC value of 3.12 μg/mL against Bacillus, E. coli, S. aureus and Klebsiella and 6.25 μg/mL against C. albicans. The ADME properties as calculated by using Qikprop were found within acceptable range. Derivatives shows a good-moderate binding affinity towards target Cytochrome P450 14 alpha-sterol demethylase (CYP51) (PDB ID: 1EA1). Conclusion: Our in-silico and in-vitro studies on a series of substituted aminobenzothiazoles may be helpful for further designing of more potent antimicrobials in future.

Microbial study of organisms isolated from nutritional fruit juices surrounded by local fruit market in Nanded, Maharashtra, India

Deshmukh AS, Siddiqui MM, Pathan UK, Dhuldhaj UP. 2020. Microbial study of organisms isolated from nutritional fruit juices surrounded by local fruit market in Nanded, Maharashtra, India. Biodiversitas 21: 4240-4246. Growing populations depend on the various food products for their nutritional values, but the hidden hunger for nutrients and vitamins are fulfilled by the fruits and their juices. Fruit juices are one of the most dependable food products, available in the Indian markets. But, standard and quality of juices always matters, hence most suggested one are freshly prepared juices as processed and packed one are mostly contains artificial flavors and food additives in the form of preservatives. Even these freshly prepared juices by local staler are also not free from contaminations. Hence, in this study we focus on the quality of fruit juices sold by the local seller. For this purpose, we collected 5 samples of fruit juices from the premises of Swami Ramanand Teerth Marathwada University, Nanded. The fruit juices are on orange, chiku, banana, apple and grapes. These samples were maintained in the basal media nutrient broth and uniculture of bacteria were isolated and maintained in the slant agar for further experiments. Total microbial load was calculated from collected and it was found that these juice samples contain significant bacterial load (2.5 x 106cfu/mL) that can cause diseases. In further investigations and identifications through the biochemical tests, we found that these juices contaminated with coliforms like E. coli and Klebsiella, along with this we also detected the presence of Listeria spp and Staphylococcus in juices samples.

Comparative Biological Activity Profile of Nava (Freshly Collected) and Purana (Old) Dhanyaka (Coriandrum sativum Linn.) through Gas Chromatography and In-Vitro Anti-Microbial Study

Introduction: Dhanyaka(Coriandrum sativum Linn.) belonging to family Apiaceae is one such common aromatic edible herb, the fruits of which are used as carminative, antimicrobial and cooling. Classical texts of Ayurveda advocate these fruits to be used in old condition. With this background, a study was done to compare biological activity profile of Nava and Purana Dhanyaka(Coriandrum sativum Linn.) through, Gas chromatography and in-vitro anti-microbial activity. Materials and methods: Fresh fruits of Dhanyaka(Coriandrum sativum Linn.) were collected from Rona, Gadag district and authenticated. The fresh fruits named as S1 with which essential oil extracted using clevengers apparatus. Few fruits were kept in an air tight container and stored for one year, and at the end of a year named as sample(S2), essential oil extracted and stored. Both samples were subjected for GC and in-vitro antimicrobial activity. Results and Discussion: The Essential oil of Nava Dhanyaka(S1) included 17 identified compounds which was dominated by monoterpenes and sesquiterpene; whereas S2 showed 6 identified compounds in which 3 constituents isoborneol, citronellyl acetate and geraniol were new findings. In-vitro anti-microbial study of essential oil extract showed that Purana Dhanyaka(S2) had better efficacy with respect to the standard against the strain Escherichia coli and Candida albicans; whereas that of Nava Dhanyaka(S1) against only one bacterial strain ie., Streptococcus pyogens. Conclusion: Preservation of Dhanyaka(Coriandrum sativum Linn.) showed biological changes in terms of antimicrobial activity compared to fresh sample. Key words: Dhanyaka, Coriandrum sativum Linn., Gas chromatography, In-vitro antimicrobial study)