Sample Collection Device Methods for Gases and Vapors

2004 ◽  
pp. 161-207 ◽  
Keyword(s):  
Sample Collection ◽  
Collection Device

scholarly journals Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1019
Author(s):  
Kyungjin Hong ◽  
Gabriella Iacovetti ◽  
Ali Rahimian ◽  
Sean Hong ◽  
Jon Epperson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
Sample Collection ◽  
Rapid Separation ◽  
Cell Counts ◽  
Collection Device ◽  
Precision Study ◽  
Clinically Significant ◽  
Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

Clinical application of volumetric absorptive microsampling to the gefapixant development program

Bioanalysis ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 893-904 ◽  
Author(s):  
Brad Roadcap ◽  
Azher Hussain ◽  
Dan Dreyer ◽  
Keynu Carter ◽  
Neal Dube ◽  
...  
Keyword(s):  
Development Program ◽  
P2x3 Receptor ◽  
Interaction Study ◽  
Sample Collection ◽  
Mathematical Relationship ◽  
Drug Interaction Study ◽  
Bioanalytical Method ◽  
Collection Device ◽  
Plasma Assay ◽  
Volumetric Absorptive Microsampling

In this paper we show the application of the Tasso OnDemand™, a novel automated sample collection device, in conjunction with volumetric absorptive microsampling (VAMS) for the development of gefapixant, a P2X3 receptor antagonist currently under clinical development for the treatment of refractory and unexplained chronic cough and endometriosis-related pain. A LC–MS/MS bioanalytical method was developed and validated using VAMS to support this development program. This method was utilized in a drug–drug interaction study to establish a mathematical bridging relationship with data obtained from a validated plasma assay used to support the program. The VAMS bioanalytical method and the predictability of the mathematical relationship is reported and discussed here.

scholarly journals Active Chemical Sampling Using Jet Discharge Inspired by Crayfish: CFD Simulations of the Flow Fields Generated by the Jet Discharge Device

Sensors ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 522 ◽  
Author(s):  
Hanako Ishida ◽  
Ryuichi Takemura ◽  
Tatsuki Mitsuishi ◽  
Haruka Matsukura ◽  
Hiroshi Ishida
Keyword(s):  
Flow Fields ◽  
Sample Collection ◽  
Cfd Simulations ◽  
Surrounding Water ◽  
Water Currents ◽  
Discharge Device ◽  
Collection Device ◽  
Active Chemical ◽  
Computational Fluid Dynamics Cfd ◽  
Solute Species

Here, we report on computational fluid dynamics (CFD) simulations conducted to develop a chemical sample collection device inspired by crayfish. The sensitivity of chemical sensors can be improved when used with a sniffing device. By collecting fluid samples from the surroundings, all solute species are also collected for the sensor. Crayfish generate jet-like water currents for this purpose. Compared to simply sucking water, food smells dissolved in the surrounding water can be more efficiently collected using the inflow induced by the jet discharge because of the smaller decay of the inflow velocity with the distance. Moreover, the angular range of water sample collection can be adjusted by changing the directions of the jet discharge. In our previous work, a chemical sample collection device that mimics the jet discharge of crayfish has been proposed. Here, we report CFD simulations of the flow fields generated by the device. By carefully configuring the simulation setups, we have obtained simulation results in which the angular ranges of the chemical sample collection in real experiments is well reproduced. Although there are still some discrepancies between the simulation and experimental results, such simulations will facilitate the process of designing such devices.

Use of a new sample-collection device (Macroduct) in anion analysis of human sweat.

1986 ◽  
Vol 32 (7) ◽  
pp. 1375-1378 ◽  
Author(s):  
D E Cole ◽  
M J Boucher
Keyword(s):  
Sweat Rate ◽  
Sulfate Concentration ◽  
Sample Collection ◽  
Human Sweat ◽  
Sweat Chloride ◽  
Collection Device ◽  
Trace Constituents ◽  
Source Of Error ◽  
Chloride Concentrations ◽  
Inorganic Sulfate

Abstract We compared concentrations of chloride and sulfate in sweat obtained by use of the Macroduct capillary-coil collection device with results obtained by the conventional absorbent filter pad technique. Samples obtained with the device weighed less than those obtained conventionally, but sweat chloride concentrations were not significantly different. To assess analysis of trace anions, we used inorganic sulfate as a prototype. Background contamination, a problem with the filter pads, was negligible with the Macroduct collector. However, with the Macroduct device, sulfate concentrations were nominally higher than with the conventional pads (105 +/- 6 vs 88 +/- 5 mumol/L) and showed no dependence of sulfate concentration on sweat rate. Subtraction of the significant "background" SO4 concentration obtained with blanks (i.e., unused filter pads) is a likely source of error in the conventional method. We consider the Macroduct device useful for study of trace constituents of human sweat.

Impact of Preanalytical Factors on Fecal Immunochemical Tests: Need for New Strategies in Comparison of Methods

2015 ◽  
Vol 30 (3) ◽  
pp. 269-274 ◽  
Author(s):  
Tiziana Rubeca ◽  
Filippo Cellai ◽  
Massimo Confortini ◽  
Callum G. Fraser ◽  
Stefano Rapi
Keyword(s):  
Information Acquisition ◽  
Significant Contribution ◽  
Correlation Coefficients ◽  
Sample Collection ◽  
Screening Programs ◽  
Standard Procedures ◽  
Collection Device ◽  
European Markets ◽  
Analytical Variation ◽  
New Strategies

Background Harmonization of fecal immunochemical tests for hemoglobin (FIT-Hb) is crucial to compare clinical outcomes in screening programs. The lack of reference materials and standard procedures does not allow the use of usual protocols to compare methods. We propose 2 protocols, based on artificial biological samples (ABS), to discriminate preanalytical and analytical variation and investigate clinical performances. The protocols were used to compare 2 FIT systems available on European markets: the OC-Sensor Diana (Eiken, Tokyo, Japan) and HM-JACKarc (Kyowa-Medex, Tokyo, Japan). Methods ABS were obtained adding Hb to Hb-free feces. In the first procedure, 35 ABS were collected for each collection device and analyzed on both systems. In the second, 188 ABS (106 positive and 82 negative) were ­collected and tested on the specific systems. Passing-Bablock (PB), Pearson's correlation coefficients (R) and Bland-Altman difference analysis were used to compare data. Results PB, R and mean standard errors for Bland-Altman analysis (Diana vs. Arc) results were 0.93x-0.56: R = 0.97 and 19%; and 1.09x + 5.60: R = 0.96 and −18%; for Diana and Arc devices, respectively. No correlations and no difference in positive/negative assessment were observed with the second protocol. Conclusions A good correlation was observed in comparing data generated using collection devices on the 2 systems. Manufacturers have developed different sample collection procedures for feces: therefore, data from different systems cannot easily be compared. Adoption of protocols to discriminate preanalytical and analytical variation would be a significant contribution to harmonization of FIT, facilitating data comparison and information acquisition for sample collection strategy and effect of buffers on systems.

scholarly journals The Use of Saliva as a Diagnostic Specimen for SARS CoV-2 Molecular Diagnostic Testing for Pediatric Patients

2020 ◽  
Author(s):  
Meghan Delaney ◽  
Joelle Simpson ◽  
Bobbe Thomas ◽  
Christal Ralph ◽  
Michael Evangalista ◽  
...  
Keyword(s):  
Diagnostic Testing ◽  
Molecular Diagnostic ◽  
Test Results ◽  
Sample Collection ◽  
Specimen Collection ◽  
Collection Device ◽  
Viral Transport Medium ◽  
Study Population ◽  
Saliva Collection ◽  
Pcr Assays

ABSTRACTBackgroundChildren are an important population to test for COVID-19 infection, particularly because they may shed the virus without displaying symptoms. Testing children for COVID-19 via sensitive molecular methods is important, although collecting nasopharyngeal (NP) specimens can be challenging. A less invasive mode of specimen collection that yields test results comparable to those from NP specimens would be beneficial to simplify sample collection.MethodsTo demonstrate that saliva is a suitable specimen for collection from children, the clinical usability/acceptability and the analytic performance of saliva were compared to NP specimens suspended in viral transport medium. Four different FDA EUA-approved real-time RT-PCR assays and one EUA approved saliva collection device were investigated.ResultsThe study population included 526 patients between the ages of 3 and 61 years, 461 (88%) were <18 years, 425 were asymptomatic (81.1%), 92 were symptomatic (17.6%). Saliva mixed with saliva stabilizing buffer was found to yield comparable sensitivity to NP specimens when tested on the AllPlex SARS-CoV-2 molecular test (Seegene Inc). The analytic sensitivity of the AllPlex assay during testing of spiked saliva mixed with SpectrumDNA saliva stabilizer was found to be 250 genomic copies/mL.ConclusionsOf the four FDA EUA-approved SARS-CoV-2 PCR assays studied, we found the AllPlex assay to be best suited for testing saliva specimens collected from children 5 years of age or older. The sensitivity of viral detection was equivalent to NP specimens when saliva specimens were mixed with the saliva stabilizer.

Evaluation of sample stability for a quantitative faecal immunochemical test and comparison of two sample collection approaches

2018 ◽  
Vol 55 (6) ◽  
pp. 657-664 ◽  
Author(s):  
Samantha Mellen ◽  
Maria de Ferrars ◽  
Claire Chapman ◽  
Sarah Bevan ◽  
James Turvill ◽  
...  
Keyword(s):  
Room Temperature ◽  
False Negative ◽  
Haemoglobin Concentration ◽  
Sample Collection ◽  
Negative Results ◽  
Collection Device ◽  
Sample Stability ◽  
Haemoglobin Measurement ◽  
Standard Collection ◽  
The Stability

Background Faecal immunochemical testing is increasingly being used to triage symptomatic patients for suspected colorectal cancer. However, there are limited data on the effect of preanalytical factors on faecal haemoglobin when measured by faecal immunochemical testing. The aim of this work was to evaluate the stability of faecal haemoglobin in faeces and to compare two methods of faecal haemoglobin sampling for faecal immunochemical testing. Methods Six patients provided faeces for faecal haemoglobin measurement which were transferred into specialized collection devices at baseline and at 1, 2, 3 and 7 days after storage at either room temperature or 4°C. A total of 137 patients returned both faeces transferred into the specialized collection device and faeces in a standard collection pot. A quantitative immunoturbidometric method was used to measure faecal haemoglobin and results were compared categorically. Discrepant results were assessed against diagnosis. Results Faecal haemoglobin concentration declined rapidly within a day of storage at room temperature but results remained ≥10 μg Hb/g faeces in 5/6 patients after two days. A faecal haemoglobin result ≥10 μg Hb/g faeces was obtained in 4/6 patients after storage for seven days at 4°C. Results obtained when patients used specialized collection devices were significantly different from results obtained when faeces was transferred into the specialized collection device in the laboratory. Conclusion There is considerable heterogeneity in the sample stability of faecal haemoglobin; therefore, samples should be transferred rapidly into specialized collection devices to prevent false-negative results. Use of collection devices by patients can lead to false-positive results compared with their use in a laboratory.

Sign in / Sign up

Export Citation Format

Share Document