DELLA proteins physically interact with CONSTANS to regulate flowering under long days inArabidopsis

Because environmentally degrading inorganic fertilizer use underlies current worldwide cereal yields, future agricultural sustainability demands enhanced nitrogen use efficiency. We found that genome-wide promotion of histone H3 lysine 27 trimethylation (H3K27me3) enables nitrogen-induced stimulation of rice tillering: APETALA2-domain transcription factor NGR5 (NITROGEN-MEDIATED TILLER GROWTH RESPONSE 5) facilitates nitrogen-dependent recruitment of polycomb repressive complex 2 to repress branching-inhibitory genes via H3K27me3 modification. NGR5 is a target of gibberellin receptor GIBBERELLIN INSENSITIVE DWARF1 (GID1)–promoted proteasomal destruction. DELLA proteins (characterized by the presence of a conserved aspartate-glutamate-leucine-leucine-alanine motif) competitively inhibit the GID1-NGR5 interaction and explain increased tillering of green revolution varieties. Increased NGR5 activity consequently uncouples tillering from nitrogen regulation, boosting rice yield at low nitrogen fertilization levels. NGR5 thus enables enhanced nitrogen use efficiency for improved future agricultural sustainability and food security.

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Arabidopsis MYC2 Interacts with DELLA Proteins in Regulating Sesquiterpene Synthase Gene Expression

The Plant Cell ◽

10.1105/tpc.112.098749 ◽

2012 ◽

Vol 24 (6) ◽

pp. 2635-2648 ◽

Cited By ~ 252

Author(s):

Gao-Jie Hong ◽

Xue-Yi Xue ◽

Ying-Bo Mao ◽

Ling-Jian Wang ◽

Xiao-Ya Chen

Keyword(s):

Gene Expression ◽

Sesquiterpene Synthase ◽

Della Proteins

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COP1 destabilizes DELLA proteins inArabidopsis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1907969117 ◽

2020 ◽

Vol 117 (24) ◽

pp. 13792-13799 ◽

Cited By ~ 6

Author(s):

Noel Blanco-Touriñán ◽

Martina Legris ◽

Eugenio G. Minguet ◽

Cecilia Costigliolo-Rojas ◽

María A. Nohales ◽

...

Keyword(s):

Ubiquitin Ligase ◽

E3 Ubiquitin Ligase ◽

Plant Cells ◽

Transcriptional Regulators ◽

Growth Responses ◽

Warm Temperature ◽

Della Proteins ◽

The Stability ◽

Dependent Pathway

DELLA transcriptional regulators are central components in the control of plant growth responses to the environment. This control is considered to be mediated by changes in the metabolism of the hormones gibberellins (GAs), which promote the degradation of DELLAs. However, here we show that warm temperature or shade reduced the stability of a GA-insensitive DELLA allele inArabidopsis thaliana. Furthermore, the degradation of DELLA induced by the warmth preceded changes in GA levels and depended on the E3 ubiquitin ligase CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). COP1 enhanced the degradation of normal and GA-insensitive DELLA alleles when coexpressed inNicotiana benthamiana.DELLA proteins physically interacted with COP1 in yeast, mammalian, and plant cells. This interaction was enhanced by the COP1 complex partner SUPRESSOR OFphyA-1051 (SPA1). The level of ubiquitination of DELLA was enhanced by COP1 and COP1 ubiquitinated DELLA proteins in vitro. We propose that DELLAs are destabilized not only by the canonical GA-dependent pathway but also by COP1 and that this control is relevant for growth responses to shade and warm temperature.

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The Transcription Factor INDUCER OF CBF EXPRESSION1 Interacts with ABSCISIC ACID INSENSITIVE5 and DELLA Proteins to Fine-Tune Abscisic Acid Signaling during Seed Germination in Arabidopsis

The Plant Cell ◽

10.1105/tpc.18.00825 ◽

2019 ◽

Vol 31 (7) ◽

pp. 1520-1538 ◽

Cited By ~ 7

Author(s):

Yanru Hu ◽

Xiao Han ◽

Milian Yang ◽

Minghui Zhang ◽

Jinjing Pan ◽

...

Keyword(s):

Transcription Factor ◽

Abscisic Acid ◽

Seed Germination ◽

Abscisic Acid Signaling ◽

Della Proteins ◽

Fine Tune

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DELLA proteins modulateArabidopsisdefences induced in response to caterpillar herbivory

Journal of Experimental Botany ◽

10.1093/jxb/ert420 ◽

2014 ◽

Vol 65 (2) ◽

pp. 571-583 ◽

Cited By ~ 25

Author(s):

Zhiyi Lan ◽

Sebastian Krosse ◽

Patrick Achard ◽

Nicole M. van Dam ◽

Jacqueline C. Bede

Keyword(s):

Della Proteins ◽

Caterpillar Herbivory

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Gibberellin Signaling Repressor LlDELLA1 Controls the Flower and Pod Development of Yellow Lupine (Lupinus luteus L.)

International Journal of Molecular Sciences ◽

10.3390/ijms21051815 ◽

2020 ◽

Vol 21 (5) ◽

pp. 1815

Author(s):

Katarzyna Marciniak ◽

Krzysztof Przedniczek

Keyword(s):

Expression Profiles ◽

Transcript Level ◽

Lupinus Luteus ◽

Flower Bud ◽

Precise Control ◽

Della Proteins ◽

Fruit Setting ◽

Pod Development ◽

Yellow Lupine ◽

Insight Into

Precise control of generative organ development is of great importance for the productivity of crop plants, including legumes. Gibberellins (GAs) play a key role in the regulation of flowering, and fruit setting and development. The major repressors of GA signaling are DELLA proteins. In this paper, the full-length cDNA of LlDELLA1 gene in yellow lupine (Lupinus luteus L.) was identified. Nuclear-located LlDELLA1 was clustered in a second phylogenetic group. Further analyses revealed the presence of all conserved motifs and domains required for the GA-dependent interaction with Gibberellin Insensitive Dwarf1 (GID1) receptor, and involved in the repression function of LlDELLA1. Studies on expression profiles have shown that fluctuating LlDELLA1 transcript level favors proper flower and pod development. Accumulation of LlDELLA1 mRNA slightly decreases from the flower bud stage to anther opening (dehiscence), while there is rapid increase during pollination, fertilization, as well as pod setting and early development. LlDELLA1 expression is downregulated during late pod development. The linkage of LlDELLA1 activity with cellular and tissue localization of gibberellic acid (GA3) offers a broader insight into the functioning of the GA pathway, dependent on the organ and developmental stage. Our analyses provide information that may be valuable in improving the agronomic properties of yellow lupine.

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