Protein-Lipid Interactions in the Formation of Raft Microdomains in Biological Membranes

2006 ◽  
pp. 305-336 ◽  
Author(s):  
Akihiro Kusumi ◽  
Kenichi Suzuki ◽  
Junko Kondo ◽  
Nobuhiro Morone ◽  
Yasuhiro Umemura
Keyword(s):  
Biological Membranes ◽  
Lipid Interactions

Protein-lipid interactions: paparazzi hunting for snap-shots

2009 ◽  
Vol 390 (8) ◽  
Author(s):  
Per Haberkant ◽  
Gerrit van Meer
Keyword(s):  
Membrane Proteins ◽  
Click Chemistry ◽  
Biological Samples ◽  
Biological Membranes ◽  
Lipid Interactions ◽  
Recent Introduction

Abstract Photoactivatable groups meeting the criterion of minimal perturbance allow the investigation of interactions in biological samples. Here, we review the application of photoactivatable groups in lipids enabling the study of protein-lipid interactions in (biological) membranes. The chemistry of various photoactivatable groups is summarized and the specificity of the interactions detected is discussed. The recent introduction of ‘click chemistry’ in photocrosslinking of membrane proteins by photo-activatable lipids opens new possibilities for the analysis of crosslinked products and will help to close the gap between proteomics and lipidomics.

Phenolic compounds alter the ion permeability of phospholipid bilayers via specific lipid interactions

2021 ◽  
Vol 23 (39) ◽  
pp. 22352-22366
Author(s):  
Sheikh I. Hossain ◽  
Suvash C. Saha ◽  
Evelyne Deplazes
Keyword(s):  
Phenolic Compounds ◽  
Biological Membranes ◽  
Phospholipid Bilayers ◽  
Ion Permeability ◽  
Lipid Interactions ◽  
Specific Lipid

How phenolic compounds interact with biological membranes and alter the menbrane properties.

A review of traditional and emerging methods to characterize lipid–protein interactions in biological membranes

2015 ◽  
Vol 7 (17) ◽  
pp. 7076-7094 ◽  
Author(s):  
Chih-Yun Hsia ◽  
Mark J. Richards ◽  
Susan Daniel
Keyword(s):  
Plasma Membrane ◽  
Membrane Protein ◽  
Protein Interactions ◽  
Protein Structures ◽  
Biological Membranes ◽  
Biological Functions ◽  
Cell Plasma Membrane ◽  
Lipid Interactions ◽  
Cell Plasma ◽  
Lipid Protein Interactions

Lipid–protein interactions are essential for modulating membrane protein structures and biological functions in the cell plasma membrane. In this review we describe the salient features of classical and emerging methodologies for studying protein–lipid interactions and their limitations.

scholarly journals AFM Imaging of Lipid Domains in Model Membranes

2003 ◽  
Vol 3 ◽  
pp. 59-74 ◽  
Author(s):  
Pierre Emmanuel Milhiet ◽  
Marie-Cecile Giocondi ◽  
Christian Le Grimellec
Keyword(s):  
Biological Membranes ◽  
Model Membranes ◽  
Current View ◽  
Lipid Domains ◽  
Force Microscopy ◽  
Lipid Interactions ◽  
Atomic Force ◽  
Lipid Mixtures ◽  
Afm Imaging ◽  
Kinetics Of Formation

Characterization of the two-dimensional organization of biological membranes is one of the most important issues that remains to be achieved in order to understand their structure-function relationships. According to the current view, biological membranes would be organized in in-plane functional microdomains. At least for one category of them, called rafts, the lateral segregation would be driven by lipid-lipid interactions. Basic questions like the size, the kinetics of formation, or the transbilayer organization of lipid microdomains are still a matter of debate, even in model membranes. Because of its capacity to image structures with a resolution that extends from the molecular to the microscopic level, atomic force microscopy (AFM) is a useful tool for probing the mesoscopic lateral organization of lipid mixtures. This paper reviews AFM studies on lateral lipid domains induced by lipid-lipid interactions in model membranes.

scholarly journals Dataset of the construction and characterization of stable biological nanoparticles

2020 ◽  
Author(s):  
Romina A. Gisonno ◽  
M. Alejandra Tricerri ◽  
Marina C. Gonzalez ◽  
Horacio A. Garda ◽  
Nahuel A. Ramella ◽  
...  
Keyword(s):  
Experimental Design ◽  
Organic Compounds ◽  
Protein Structures ◽  
Biological Membranes ◽  
Structural Flexibility ◽  
Chemical Crosslinking ◽  
Lipid Interactions ◽  
Excellent Model

AbstractWe suggest that the structural flexibility is key for certain proteins in order to fulfill functions that are required to interact with biological membranes, and that intra-chain chemical crosslinking may result in a different arrangement of protein with lipids. As interaction with biological membranes and lipids is a function attributed to many proteins in circulation, we intended to characterize an experimental design that helps in the study of many biological protein structures and their function. But in addition, by introducing intra-chain crosslinking, we obtained discoidal nano platforms that are stable under different conditions of temperate and time incubation. These platforms might be an excellent model to employ as biological carriers of intrinsic or external molecules. Thus, data shown here clearly strengthen the usefulness of an easy, accessible and inexpensive tool not only to study protein-lipid interactions, but to be used in different biological fields that require the transport of organic compounds.

Electron Diffraction of Wet Biological Membranes

1974 ◽  
Vol 32 ◽  
pp. 158-159
Author(s):  
S.W. Hui ◽  
D.F. Parsons
Keyword(s):  
Electron Diffraction ◽  
Data Collection ◽  
Biological Membranes ◽  
Small Areas ◽  
Electron Diffraction Technique ◽  
Electron Microscopes ◽  
Collection Time ◽  
Camera Length

The development of the hydration stages for electron microscopes has opened up the application of electron diffraction in the study of biological membranes. Membrane specimen can now be observed without the artifacts introduced during drying, fixation and staining. The advantages of the electron diffraction technique, such as the abilities to observe small areas and thin specimens, to image and to screen impurities, to vary the camera length, and to reduce data collection time are fully utilized. Here we report our pioneering work in this area.

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

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