scholarly journals Live‐Cell Imaging: Time‐Gated FRET Nanoprobes for Autofluorescence‐Free Long‐Term In Vivo Imaging of Developing Zebrafish (Adv. Mater. 39/2020)

2020 ◽  
Vol 32 (39) ◽  
pp. 2070291 ◽  
Author(s):  
Marcelina Cardoso Dos Santos ◽  
Ingrid Colin ◽  
Gabriel Ribeiro Dos Santos ◽  
Kimihiro Susumu ◽  
Michaël Demarque ◽  
...  
2020 ◽  
Vol 2 (4) ◽  
pp. 1590-1602
Author(s):  
Geoffrey Prévot ◽  
Talia Bsaibess ◽  
Jonathan Daniel ◽  
Coralie Genevois ◽  
Guillaume Clermont ◽  
...  

Tailor-made NIR emitting dyes were designed as multimodal optical probes.


2021 ◽  
Vol 12 (1) ◽  
Author(s):  
René Schneider ◽  
Kris van’t Klooster ◽  
Kelsey L. Picard ◽  
Jasper van der Gucht ◽  
Taku Demura ◽  
...  

AbstractPlants are the tallest organisms on Earth; a feature sustained by solute-transporting xylem vessels in the plant vasculature. The xylem vessels are supported by strong cell walls that are assembled in intricate patterns. Cortical microtubules direct wall deposition and need to rapidly re-organize during xylem cell development. Here, we establish long-term live-cell imaging of single Arabidopsis cells undergoing proto-xylem trans-differentiation, resulting in spiral wall patterns, to understand microtubule re-organization. We find that the re-organization requires local microtubule de-stabilization in band-interspersing gaps. Using microtubule simulations, we recapitulate the process in silico and predict that spatio-temporal control of microtubule nucleation is critical for pattern formation, which we confirm in vivo. By combining simulations and live-cell imaging we further explain how the xylem wall-deficient and microtubule-severing KATANIN contributes to microtubule and wall patterning. Hence, by combining quantitative microscopy and modelling we devise a framework to understand how microtubule re-organization supports wall patterning.


2015 ◽  
Vol 44 (12) ◽  
pp. 5763-5770 ◽  
Author(s):  
Shyamaprosad Goswami ◽  
Krishnendu Aich ◽  
Sangita Das ◽  
Chitrangada Das Mukhopadhyay ◽  
Deblina Sarkar ◽  
...  

A new quinoline based sensor was developed and applied for the selective detection of Cd2+ both in vitro and in vivo.


2020 ◽  
Vol 343 ◽  
pp. 108808 ◽  
Author(s):  
Margarita Arango-Lievano ◽  
Yann Dromard ◽  
Pierre Fontanaud ◽  
Chrystel Lafont ◽  
Patrice Mollard ◽  
...  

2019 ◽  
Vol 55 (33) ◽  
pp. 4841-4844 ◽  
Author(s):  
Guilin Chen ◽  
Zaicai Zhou ◽  
Hui Feng ◽  
Chenyan Zhang ◽  
Yifan Wang ◽  
...  

A molecular probe with aggregation-induced phosphorescence (AIP) properties for calcium ion-specific detection and imaging in vivo was designed.


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