Hepatitis a virus immunoglobulin m antibody in acute neurological disease

1983 ◽  
Vol 14 (6) ◽  
pp. 685-687 ◽  
Author(s):  
Vivian V. Bosch ◽  
Peter C. Dowling ◽  
Stuart D. Cook
Keyword(s):  
Neurological Disease ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Immunoglobulin M

Serodiagnosis of viral hepatitis A by a modified competitive binding radioimmunoassay for immunoglobulin M anti-hepatitis A virus

1979 ◽  
Vol 9 (1) ◽  
pp. 120-127
Author(s):  
D W Bradley ◽  
H A Fields ◽  
K A McCaustland ◽  
J E Maynard ◽  
R H Decker ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Protein A ◽  
Competitive Binding ◽  
Immunoglobulin M ◽  
Test Results ◽  
Solid Phase Radioimmunoassay ◽  
Viral Hepatitis A

A competitive binding radioimmunoassay (CBA) for antibody to hepatitis A virus (HAV) was evaluated and compared with a standard solid-phase radioimmunoassay for anti-HAV, CBA was found to be sensitive and specific for the detection of anti-HAV, as demonstrated by the 98% concordance of CBA and solid-phase radioimmunoassay test results. The standard CBA test was modified for the differential detection of acute (immunoglobulin M) and convalescent (immunoglobulin G) anti-HAV by incorporation of a step in which immunoglobulin G anti-HAV was preferentially absorbed with S. aureus cells (protein A). The modified CBA test was shown to be capable of differentiating between acute- and convalescent-phase sera. The modified CBAM test was able to detect immunoglobulin M anti-HAV up to approximately 4 weeks after the onset of illness.

scholarly journals Increasing incidence of hepatitis A in Bangladesh

2012 ◽  
Vol 47 (3) ◽  
pp. 309-312 ◽  
Author(s):  
MZ Amin ◽  
LN Siddique ◽  
MA Slatter ◽  
KK Biswas
Keyword(s):  
Liver Disease ◽  
High Risk ◽  
Chronic Liver Disease ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Age Groups ◽  
Hepatitis E ◽  
Immunoglobulin M ◽  
Chronic Liver ◽  
High Risk Population

Hepatitis A (HAV) infection is caused by the hepatitis A virus which is transmitted through the fecal-oral route. Life long protective antibodies are present after infection. The number of cases of adult hepatitis A has progressively been increasing during the last several decades in Bangladesh. In addition, the pattern of age-specific seroprevalence of anti-HAV has changed with economic growth. The prevalence of anti-HAV in 20-40 year age range has declined rapidly during the last 3 decades. As a result, this age groups has a high risk for HAV infection and clinically overt hepatitis A is increasing in adolescents and adult. The aim of the present study were to assess whether the proportion of adults with acute HAV infection has been increasing over the years and analyze the seroprevalence of immunoglobulin M(IgM) anti- HAV antibodies in young adults below the age of 20 years as well as in cases of chronic liver disease. Sera collected from 530 patients with acute and chronic liver disease attends the Somorita Hospital Ltd. during the previous 2 years and 6 months (Jan. 2008- Jun. 2010) were tested for various serological markers of acute and chronic hepatitis. In addition, 530 normal healthy attendants of the patients above the age of 20 years were tested for IgM anti-HAV as controls. Of 530 patients with acute hepatitis (13.42%) were positive for immunoglobulin M. The patients who were IgM anti-HAV negative were found to be hepatitis B (106 patients), hepatitis C, (10 patients), hepatitis E (150 patients) and unclassified (273 patients). Although the frequency of HAV infection among young adult (< 20 age) had increased (33.33% to 42.35%) in the 2 years and 6 months period, the frequency of HAV infection among adults had also increased (15.38% to 28.13%) during the same period. This study should be helpful for the identification of high risk population for vaccination of hepatitis A. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13065 Bangladesh J. Sci. Ind. Res. 47(3), 309-312 2012

Shift in hepatitis a epidemiology in Germany: Population distribution of hepatitis a virus antibodies of the immunoglobulin M class

Infection ◽  
1980 ◽  
Vol 8 (6) ◽  
pp. 262-266 ◽  
Author(s):  
M. Roggendorf ◽  
G. Panitz ◽  
R. Scheid ◽  
B. Bayerl ◽  
G. G. Frösner ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Population Distribution ◽  
Hepatitis A Virus ◽  
Immunoglobulin M

Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies.

1993 ◽  
Vol 31 (5) ◽  
pp. 1299-1302 ◽  
Author(s):  
P L Summers ◽  
D R Dubois ◽  
W H Cohen ◽  
P O Macarthy ◽  
L N Binn ◽  
...  
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Antibody Capture

Multiple Factors Contribute to Positive Results for Hepatitis A Virus Immunoglobulin M Antibody

2013 ◽  
Vol 137 (1) ◽  
pp. 90-95 ◽  
Author(s):  
Adnan Alatoom ◽  
M. Qasim Ansari ◽  
Jennifer Cuthbert
Keyword(s):  
Liver Disease ◽  
Acute Hepatitis ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Laboratory Data ◽  
The United States ◽  
Immunoglobulin M ◽  
Order Entry ◽  
Igm Antibody ◽  
Positive Results

Context.—In the United States, a successful vaccination program for hepatitis A virus (HAV) infection has decreased both its incidence and the true positive rate for diagnostic immunoglobulin M (IgM) antibody to HAV in acute hepatitis. Objective.—To survey positive results of HAV IgM tests and determine the effect of changing ordering options. Design.—We reviewed all positive results for IgM antibody to HAV between January 2007 and December 2010. Patient demographics, clinical history, and laboratory data were recorded and the encounter, order, and reason for test reviewed. Each result was categorized as indicating acute, recent, resolved, or indeterminate HAV infection. Results.—A total of 10 735 tests were performed; 35 patients had 49 positive results. Most positive test results were associated with outpatient visits and were ordered in the assessment of patients with liver disease, but not clinical acute hepatitis. In the final analysis, 4 patients had acute hepatitis A and 20 individual patients had recent and/or resolved hepatitis. All but 1 of the remaining 11 patients had another established cause of liver disease with a positive IgM HAV antibody test result; data to determine causality were insufficient. The total number of tests requested annually decreased more than 35% with the introduction of computerized physician order entry. Conclusions.—Current assays for IgM HAV antibodies are overused in the absence of clinical acute hepatitis; future clinical decision support may improve patterns of order entry. Most patients have findings consistent with HAV exposure but not acute hepatitis; dormant viral infection may be a continuing source of antigen.

scholarly journals Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks

2003 ◽  
Vol 10 (3) ◽  
pp. 492-494 ◽  
Author(s):  
Licel de los Angeles Rodríguez Lay ◽  
Osmany Larralde Díaz ◽  
Raiza Martínez Casanueva ◽  
Aidonis Gutiérrez Moreno
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rapid Diagnosis ◽  
Immunoglobulin M ◽  
Urine Samples ◽  
Test Results ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Urine And Serum

ABSTRACT The main goal of this study was to test the feasibility of using urine for diagnosing hepatitis A virus (HAV) infections. A correlation of 90.78% between the test results of urine and serum samples was obtained. Four outbreaks of hepatitis A were confirmed by testing only urine samples. The levels of anti-HAV immunoglobulin M (IgM) antibodies in urine samples remained stable during 6 months of storage at −70°C but decreased when the samples were stored at 4°C. The results of tests of samples obtained 2 and 6 months after infection suggested that IgM levels decline more rapidly in urine than in serum.

scholarly journals Nationwide seroprevalence of hepatitis A in South Korea from 2009 to 2019

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0245162
Author(s):  
Deog-Yong Lee ◽  
Su-Jin Chae ◽  
Seung-Rye Cho ◽  
Wooyoung Choi ◽  
Chang-Ki Kim ◽  
...  
Keyword(s):  
South Korea ◽  
Immunoglobulin G ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Age Groups ◽  
Immunoglobulin M ◽  
Acute Type ◽  
Prevention Policy ◽  
Patient Report ◽  
Positive Rate

Hepatitis A, an acute type of hepatitis caused by the hepatitis A virus, occurs worldwide. Following the 2009 hepatitis A epidemic in South Korea, patient outbreak reports were collectively converted to an “all-patient report” in 2011, and national immunization programs were introduced for children in 2015. In this study, we aimed to analyze the changes and characteristics of hepatitis A antibody titers in South Korea following the epidemic. The results of hepatitis A antibody tests performed at clinical laboratories from 2009 to 2019 were analyzed based on year, age, region, sex, and medical institution. The average 2009–2018 positive anti-hepatitis A virus immunoglobulin G rate was 51.8%, but it increased (56.06%) in 2019. Significantly different antibody-positive rates were observed based on age: <10 years, 54.5%; 20–29 years, 19.5%; ≥50 years, almost 100%. The positive rate of individuals in their teens and 20s gradually increased, whereas that of those in their 30s and 40s gradually decreased. Males had higher antibody-positive rates than females, and samples from higher-level general hospitals exhibited higher antibody rates. The positive anti-hepatitis A virus immunoglobulin M rates gradually decreased after 2009 and were <1% after 2012. However, a high positive rate of 3.69% was observed in 2019 when there was an epidemic. Anti-hepatitis A virus immunoglobulin G-positive rates were similar throughout the year, but the anti-hepatitis A virus immunoglobulin M-positive rates increased from January, peaked in April, and decreased from July, exhibiting distinct seasonality. This is considered to be related to groundwater pollution during the spring drought season. The introduction of the “all-patient report” and national vaccination program for children has had an effective influence on hepatitis A management. However, for hepatitis A prevention, policy considerations for high-risk age groups with low antibody-positive rates will be necessary.

scholarly journals Evaluation of Urine as a Clinical Specimen for Diagnosis of Hepatitis A

2002 ◽  
Vol 9 (4) ◽  
pp. 840-845 ◽  
Author(s):  
Madhuri S. Joshi ◽  
Shobha D. Chitambar ◽  
Vidya A. Arankalle ◽  
Mandeep S. Chadha
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Clinical Specimen ◽  
Immunoglobulin M ◽  
Control Groups ◽  
Past Infection ◽  
Significant Difference ◽  
Paired Serum ◽  
Urine Specimens ◽  
Serum And Urine

ABSTRACT The present study pertains to the evaluation of urine as a specimen for detection of anti-hepatitis A virus (anti-HAV) antibodies. Immunoglobulin M (IgM), IgG, and IgA capture enzyme-linked immunosorbent assays for hepatitis A were performed on paired serum and urine specimens collected from hepatitis A patients (n = 92), healthy individuals (n = 100), non-A hepatitis patients (n = 70), and patients with nonhepatic diseases (n = 64, including 37 renal disease patients). Hepatitis A patients seropositive for anti-HAV IgM showed 95.65% uropositivity. No false-positive reactions were observed in control groups. The uropositivity of anti-HAV IgM persisted during the convalescent phase of the disease. Anti-HAV IgG uropositivity correlated well with corresponding seropositivity in all groups (P > 0.05 for each). No significant difference between the proportions of serum and urine positivity for anti-HAV IgA was noted (P > 0.05 for each). Using seroreactivity as a “gold standard,” the sensitivity and specificity for anti-HAV IgM, anti-HAV IgG, and anti-HAV IgA tests with urine as a specimen were found to be 95.65 and 100%, 97.76 and 76.47%, and 92.23 and 88.18%, respectively. Urine appears to be comparable to serum for diagnosis of recent and past infection with hepatitis A.

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