The validation of the role of several genes related to Bombyx mori nucleopolyhedrovirus infection in vivo

2021 ◽  
Vol 106 (2) ◽  
Author(s):  
Xue‐yang Wang ◽  
Chun‐xiao Zhao ◽  
Xin Wang ◽  
Zi‐qin Zhao ◽  
Zhi‐hao Su ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals Bombyx mori nucleopolyhedrovirus BmP95 plays an essential role in budded virus production and nucleocapsid assembly

2013 ◽  
Vol 94 (7) ◽  
pp. 1669-1679 ◽  
Author(s):  
Xingwei Xiang ◽  
Yunwang Shen ◽  
Rui Yang ◽  
Lin Chen ◽  
Xiaolong Hu ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Virus Production ◽  
Full Length ◽  
Tubular Structures ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Budded Virus ◽  
Conserved Gene ◽  
Fluorescence And Light Microscopy

Bombyx mori nucleopolyhedrovirus (BmNPV) BmP95 is a highly conserved gene that is found in all of the baculovirus genomes sequenced to date and is also found in nudiviruses. To investigate the role of BmP95 in virus infection in vitro, a BmP95 deletion virus (vBmP95-De) was generated by homologous recombination in Escherichia coli. Fluorescence and light microscopy and titration analysis indicated that the BmP95 deletion bacmid led to a defect in production of infectious budded virus (BV). However, deletion of BmP95 did not affect viral DNA replication. Electron microscopy showed that masses of aberrant tubular structures were present in cells transfected with the BmP95 deletion bacmid, indicating that deletion of BmP95 affected assembly of the nucleocapsid. This defect could be rescued by insertion of full-length BmP95 into the polyhedrin locus of the BmP95-knockout bacmid but not the N-terminal domain of BmP95. Together, these results showed that full-length BmP95 is essential for BV production and is required for nucleocapsid assembly.

Role of the Bombyx mori nucleopolyhedrovirus LEF3 acetylation on viral replication

2021 ◽  
pp. 105109
Author(s):  
Xu Gao ◽  
Jihai Lei ◽  
Yajie Zhu ◽  
Xi Chen ◽  
Fuxiang Mao ◽  
...  
Keyword(s):  
Viral Replication ◽  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals In vivo and in vitro analyses of a Bombyx mori nucleopolyhedrovirus mutant lacking functional vfgf

Virology ◽  
2006 ◽  
Vol 355 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Susumu Katsuma ◽  
Satoshi Horie ◽  
Takaaki Daimon ◽  
Masashi Iwanaga ◽  
Toru Shimada
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus

scholarly journals Role of the ubiquitin-proteasome system in Bombyx mori nucleopolyhedrovirus infection

2010 ◽  
Vol 92 (3) ◽  
pp. 699-705 ◽  
Author(s):  
S. Katsuma ◽  
A. Tsuchida ◽  
N. Matsuda-Imai ◽  
W. Kang ◽  
T. Shimada
Keyword(s):  
Bombyx Mori ◽  
Ubiquitin Proteasome System ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Ubiquitin Proteasome

Inhibitory role of the Bm8 protein in the propagation of Bombyx mori nucleopolyhedrovirus

2018 ◽  
Vol 249 ◽  
pp. 124-131 ◽  
Author(s):  
Hiroyuki Hikida ◽  
Ryuhei Kokusho ◽  
Jun Kobayashi ◽  
Toru Shimada ◽  
Susumu Katsuma
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Inhibitory Role

Mitochondrial matrix calcium ions regulate energy production in myocardium: A cytochemical study

1990 ◽  
Vol 48 (2) ◽  
pp. 166-167
Author(s):  
W.A. Jacob ◽  
R. Hertsens ◽  
A. Van Bogaert ◽  
M. De Smet
Keyword(s):  
Energy Metabolism ◽  
Calcium Ions ◽  
Energy Production ◽  
Regulation Of Respiration ◽  
Free Calcium ◽  
Mitochondrial Matrix ◽  
Cytochemical Study ◽  
Nmr Techniques

In the past most studies of the control of energy metabolism focus on the role of the phosphorylation potential ATP/ADP.Pi on the regulation of respiration. Studies using NMR techniques have demonstrated that the concentrations of these compounds for oxidation phosphorylation do not change appreciably throughout the cardiac cycle and during increases in cardiac work. Hence regulation of energy production by calcium ions, present in the mitochondrial matrix, has been the object of a number of recent studies.Three exclusively intramitochondnal dehydrogenases are key enzymes for the regulation of oxidative metabolism. They are activated by calcium ions in the low micromolar range. Since, however, earlier estimates of the intramitochondnal calcium, based on equilibrium thermodynamic considerations, were in the millimolar range, a physiological correlation was not evident. The introduction of calcium-sensitive probes fura-2 and indo-1 made monitoring of free calcium during changing energy metabolism possible. These studies were performed on isolated mitochondria and extrapolation to the in vivo situation is more or less speculative.

Meiotic CENP-C is a shepherd: bridging the space between the centromere and the kinetochore in time and space

2020 ◽  
Vol 64 (2) ◽  
pp. 251-261
Author(s):  
Jessica E. Fellmeth ◽  
Kim S. McKim
Keyword(s):  
Chromosome Segregation ◽  
New Technologies ◽  
Early Prophase ◽  
Unique Role ◽  
Kinetochore Assembly ◽  
M Phase ◽  
In Vivo Analysis ◽  
Meiosis I

Abstract While many of the proteins involved in the mitotic centromere and kinetochore are conserved in meiosis, they often gain a novel function due to the unique needs of homolog segregation during meiosis I (MI). CENP-C is a critical component of the centromere for kinetochore assembly in mitosis. Recent work, however, has highlighted the unique features of meiotic CENP-C. Centromere establishment and stability require CENP-C loading at the centromere for CENP-A function. Pre-meiotic loading of proteins necessary for homolog recombination as well as cohesion also rely on CENP-C, as do the main scaffolding components of the kinetochore. Much of this work relies on new technologies that enable in vivo analysis of meiosis like never before. Here, we strive to highlight the unique role of this highly conserved centromere protein that loads on to centromeres prior to M-phase onset, but continues to perform critical functions through chromosome segregation. CENP-C is not merely a structural link between the centromere and the kinetochore, but also a functional one joining the processes of early prophase homolog synapsis to late metaphase kinetochore assembly and signaling.

The Role of Polyphenols in the Modulation of Plasma Non-Enzymatic Antioxidant Capacity (NEAC)

2012 ◽  
Vol 82 (3) ◽  
pp. 228-232 ◽  
Author(s):  
Mauro Serafini ◽  
Giuseppa Morabito
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Intervention ◽  
Ex Vivo ◽  
The Body ◽  
Dietary Polyphenols ◽  
Enzymatic Antioxidant ◽  
Endogenous Antioxidant

Dietary polyphenols have been shown to scavenge free radicals, modulating cellular redox transcription factors in different in vitro and ex vivo models. Dietary intervention studies have shown that consumption of plant foods modulates plasma Non-Enzymatic Antioxidant Capacity (NEAC), a biomarker of the endogenous antioxidant network, in human subjects. However, the identification of the molecules responsible for this effect are yet to be obtained and evidences of an antioxidant in vivo action of polyphenols are conflicting. There is a clear discrepancy between polyphenols (PP) concentration in body fluids and the extent of increase of plasma NEAC. The low degree of absorption and the extensive metabolism of PP within the body have raised questions about their contribution to the endogenous antioxidant network. This work will discuss the role of polyphenols from galenic preparation, food extracts, and selected dietary sources as modulators of plasma NEAC in humans.

Potential Uses of Vinegar as a Medicine and Related in vivo Mechanisms

2016 ◽  
Vol 86 (3-4) ◽  
pp. 127-151 ◽  
Author(s):  
Zeshan Ali ◽  
Zhenbin Wang ◽  
Rai Muhammad Amir ◽  
Shoaib Younas ◽  
Asif Wali ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Review Paper ◽  
Heart Diseases ◽  
Folk Medicine ◽  
Active Role ◽  
Current Review ◽  
Different Types ◽  
Positive Effect

While the use of vinegar to fi ght against infections and other crucial conditions dates back to Hippocrates, recent research has found that vinegar consumption has a positive effect on biomarkers for diabetes, cancer, and heart diseases. Different types of vinegar have been used in the world during different time periods. Vinegar is produced by a fermentation process. Foods with a high content of carbohydrates are a good source of vinegar. Review of the results of different studies performed on vinegar components reveals that the daily use of these components has a healthy impact on the physiological and chemical structure of the human body. During the era of Hippocrates, people used vinegar as a medicine to treat wounds, which means that vinegar is one of the ancient foods used as folk medicine. The purpose of the current review paper is to provide a detailed summary of the outcome of previous studies emphasizing the role of vinegar in treatment of different diseases both in acute and chronic conditions, its in vivo mechanism and the active role of different bacteria.

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