scholarly journals Lymphocyte depletion by continuous flow cell centrifugation in rheumatoid arthritis

1979 ◽  
Vol 22 (10) ◽  
pp. 1055-1059 ◽  
Author(s):  
Jacob Karsh ◽  
Daniel G. Wright ◽  
John H. Klippel ◽  
John L. Decker ◽  
Albert B. Deisseroth ◽  
...  
2015 ◽  
Vol 3 (1) ◽  
pp. 68-77 ◽  
Author(s):  
Evandro L. Dall'Oglio ◽  
Paulo T. de Sousa ◽  
Leonardo Gomes de Vasconcelos ◽  
Carlos Adriano Parizotto ◽  
Ewerton Ferreira Barros ◽  
...  

Blood ◽  
1974 ◽  
Vol 44 (3) ◽  
pp. 435-443 ◽  
Author(s):  
Mamoru Shoji ◽  
William R. Vogler

Abstract The usefulness of granulocyte transfusions is in part dependent upon the number of granulocytes transfused. The invention of the continuous-flow cell separator has made it possible to obtain granulocytes from normal donors. Efforts to improve the yield are under study. This controlled study was undertaken to determine the effect of a single dose of hydrocortisone on granulocyte yield from volunteer donors and on granulocyte bactericidal function. Twenty-two normal volunteers were randomized between no therapy or a single intravenous injection of 120 mg/sq m of hydrocortisone 2 hr prior to initiation of a 4-hr leukapheresis using the Aminco cell separator operated at 750 rpm and a flow rate of 41 ml/min. Significant increases in granulocyte yield and reductions in lymphocyte and monocyte yields were obtained in the hydrocortisone-treated group. Granulocytes from each group were equally effective in the phagocytosis of yeast particles and in vitro bactericidal activity.


Lab on a Chip ◽  
2018 ◽  
Vol 18 (24) ◽  
pp. 3760-3769 ◽  
Author(s):  
Xiaoxing Xing ◽  
Chun Ning Ng ◽  
Ming Lok Chau ◽  
Levent Yobas

Continuous-flow cell sorting at considerably high flow rates by railing cells along electrode tracks based on dielectrophoresis and hydrodynamic drag.


Blood ◽  
1979 ◽  
Vol 54 (1) ◽  
pp. 254-268 ◽  
Author(s):  
JP Hester ◽  
RM Kellogg ◽  
AP Mulzet ◽  
VR Kruger ◽  
KB McCredie ◽  
...  

Abstract A single-stage disposable channel and seal that provides for leukocyte and granulocyte collection by continuous-flow cell separation (CFCS) has been designed by the IBM Corporation. This paper describes (1) the separation characteristics of whole blood as it responds to varying gravitational (G) forces and flow rates through the channel; (2) the mechanism by which the buffy coat accumulates and is extracted; (3) the efficiency of extraction; (4) those donor and procedural variables that contribute to the final yield; (5) posttransfusion increment response in patients; and (6) the functional integrity of the cells collected.


PROTOPLASMA ◽  
1986 ◽  
Vol 132 (1-2) ◽  
pp. 99-106 ◽  
Author(s):  
J. Larsen ◽  
J. R. Nilsson

Transfusion ◽  
1988 ◽  
Vol 28 (5) ◽  
pp. 470-473 ◽  
Author(s):  
F Norol ◽  
C Aubert ◽  
F Scotto ◽  
N Duedari

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