scholarly journals Isolation ofYERSINIA-Specific T Cell Clones from the Synovial Membrane and Synovial Fluid of a Patient with Reactive Arthritis

1991 ◽  
Vol 34 (9) ◽  
pp. 1151-1157 ◽  
Author(s):  
N. J. Viner ◽  
L. C. Bailey ◽  
P. F. Life ◽  
P. A. Bacon ◽  
J. S. H. Gaston
Keyword(s):  
T Cell ◽  
Synovial Fluid ◽  
Reactive Arthritis ◽  
Synovial Membrane ◽  
T Cell Clones ◽  
Cell Clones

Salmonella-reactive Synovial Fluid T-cell Clones in a Patient with Post-infectious Salmonella Arthritis

1990 ◽  
Vol 19 (5) ◽  
pp. 350-355 ◽  
Author(s):  
E. Hermann ◽  
W.-J. Mayet ◽  
T. Poralla ◽  
K.-H. Meyer Zum Büschenfelde ◽  
B. Fleischer
Keyword(s):  
T Cell ◽  
Synovial Fluid ◽  
T Cell Clones ◽  
Cell Clones ◽  
Post Infectious

scholarly journals Recognition of a mycobacteria-specific epitope in the 65-kD heat-shock protein by synovial fluid-derived T cell clones.

1990 ◽  
Vol 171 (3) ◽  
pp. 831-841 ◽  
Author(s):  
J S Gaston ◽  
P F Life ◽  
P J Jenner ◽  
M J Colston ◽  
P A Bacon
Keyword(s):  
Amino Acids ◽  
T Cell ◽  
Heat Shock ◽  
Synovial Fluid ◽  
Heat Shock Protein ◽  
Mononuclear Cells ◽  
Cell Clone ◽  
T Cell Clones ◽  
Cell Clones ◽  
T Cell Clone

Adjuvant arthritis in rats is induced by a T cell clone specific for amino acids 180-188 of the mycobacterial 65-kD heat-shock protein, and synovial T cell responses to this same Ag have been noted in human arthritis. We have isolated 65-kD Ag-specific T cell clones from synovial fluid mononuclear cells of a patient with acute arthritis, which, unlike the corresponding PBMC, showed a marked proliferative response to the 65-kD Ag. Using synthetic peptides corresponding to the whole sequence of the 65-kD Ag, all the clones were shown to recognize an epitope present in the first NH2-terminal peptide (amino acids 1-15), with no response to the adjacent peptide (amino acids 6-22) or to any other peptide. The complete dominance of this epitope in the response to the 65-kD Ag was shown by documenting responses to the peptide in PBMC obtained after recovery from the arthritis. This epitope, like that recognized by the rat arthritogenic T cell clone, is in a portion of the 65-kD sequence that is not conserved between bacteria and eukaryotes, so that in this case, joint inflammation could not be attributed to bacteria-induced T cell clones cross-reacting with the self 65-kD Ag.

scholarly journals Persistence of collagen type II-specific T-cell clones in the synovial membrane of a patient with rheumatoid arthritis.

1989 ◽  
Vol 86 (2) ◽  
pp. 636-640 ◽  
Author(s):  
M. Londei ◽  
C. M. Savill ◽  
A. Verhoef ◽  
F. Brennan ◽  
Z. A. Leech ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Synovial Membrane ◽  
Collagen Type ◽  
Type Ii ◽  
Collagen Type Ii ◽  
T Cell Clones ◽  
Cell Clones

scholarly journals OP0027 AS-RELATED TCR BETA CLONOTYPES ARE PRESENT IN DIFFERENT INFLAMED TISSUES OF PATIENTS WITH SPONDYLOARTHROPATHIES

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 14.3-15
Author(s):  
E. Komech ◽  
A. Barinova ◽  
E. Shmidt ◽  
T. Korotaeva ◽  
A. Koltakova ◽  
...  
Keyword(s):  
T Cells ◽  
Psoriatic Arthritis ◽  
Ankylosing Spondylitis ◽  
T Cell ◽  
Synovial Fluid ◽  
Intestinal Inflammation ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
T Cell Clones ◽  
Cell Clones

Background:Recently a group of T-cell clones with characteristic T-cell receptor (TCR) motif was identified in peripheral blood and synovial fluid of HLA-B*27+ patients with ankylosing spondylitis (AS) [1-2] - a prototypic disease from a wider group of spondyloarthropathies (SpAs). Extraarticular manifestations of AS could involve skin, intestine or eye. Emerging data indicate linkage between intestinal and joint inflammation, including expression of gut-associated integrins on synovial T-cells [3-4]. However, clonal T-cell composition and presence of identical clones in different inflamed sites in SpAs remains poorly studied.Objectives:To investigate clonal T-cell repertoire and presence of AS-related TCR motif in different sites of inflammation of patients with SpA.Methods:Samples of synovial fluid (SF) were obtained from HLA-B*27+ and HLA-B*27- patients with ankylosing spondylitis (AS) and psoriatic arthritis (PsA), as well as gut biopsy samples from patients with AS and Crohn’s disease (AS/CD) or ulcerative colitis (AS/UC), and conjunctival swabs from patients with uveitis (Uv) and with or without articular manifestations (Table 1). Also SF and gut biopsy samples were obtained from HLA-B*27+ patients with juvenile idiopathic arthritis (JIA). For one patient PsA patient paired samples of SF and gut biopsy were obtained.Table 1.Detection of the AS-related motif TRBV9_CASS[V/A/L/P][G/A] [L/T/V][F/Y]STDTQYF_TRBJ2-3 in bTCR repertoires of samples from different inflamed sites of patients with SpATissueDiagnosisB27+B27-AS-related TCR motif+ among all samples from B27+ donorsSynovial fluidAS2012PsAJIAIntestinal biopsyAS/CD433 / 4AS/UCJLAConjunctival swabUv804 / 8SF and gut samples were processed to isolate mononuclear cells, while conjunctival swabs were directly lysed in the lysis buffer. CD3+ β7-intergin+ cells were isolated from SF by fluorescence-activated cell sorting. Deep TCR repertoire profiling was carried out using UMI-based cDNA library preparation technology [1].Results:Identical T-cell clonotypes were detected between paired SF and gut samples of the same patient with psoriatic arthritis and intestinal inflammation. The subpopulation of β7-intergin+ SF T-cells shared significantly more identical clonotypes with gut biopsy repertoire compared to the bulk SF T-cell repertoire.Clonotypes belonging to the AS-related TCR beta motif TRBV9_CASS[V/A/L/P][G/A][L/T/V][F/Y]STDTQYF_TRBJ2-3 were detected in all inflamed tissues tested: synovial fluid, intestinal biopsies and conjunctival swabs of SpA patients (Table 1). Importantly, we observed these clonotypes exclusively in samples from HLA-B*27+ donors (n=26), but not in HLA-B27- context (n=15) with comparable analysis depth, thus confirming strong HLA-B*27-restriction of the clonotypes. The AS-related clonotypes were detected in the subpopulation of β7-intergin+ SF T-cells from HLA-B*27+ patients with PsA.Conclusion:For the first time we directly report the T-cell clonal sharing between synovial fluid and inflamed gut tissue of SpA patients. In sum our data suggests involvement of identical T-cell clones in inflammation in different anatomical sites in SpA.References:[1]Komech et al. Rheumatology (Oxford). 2018;57(6):1097-1104.[2]Faham et al. Arthritis Rheumatol. 2016;11(10):300-308.[3]Guggino et al.Ann Rheum Dis. Published Online First: 18 October 2019.doi:10.1136/annrheumdis-2019-216456.[4]Qaiyum et al Ann Rheum Dis. 2019;78(11):1566-1575.Acknowledgements:We thanks all the patients and medical personnel involved in the studyDisclosure of Interests:None declared

AB0021 In Psoriatic Arthritis Synovial Tissue Harbors Expanded T-Cell Clones Which Are not Fully Represented in Synovial Fluid or Blood Samples: Table 1

2014 ◽  
Vol 73 (Suppl 2) ◽  
pp. 810.2-811
Author(s):  
A. Musters ◽  
M.E. Doorenspleet ◽  
P.L. Klarenbeek ◽  
R.E. Esveldt ◽  
D.L. Baeten ◽  
...  
Keyword(s):  
Psoriatic Arthritis ◽  
T Cell ◽  
Synovial Fluid ◽  
Synovial Tissue ◽  
Blood Samples ◽  
T Cell Clones ◽  
Cell Clones

Diversity in antigen recognition byMycobacterium tuberculosis-reactive T cell clones from the synovial fluid of rheumatoid arthritis patients

1991 ◽  
Vol 21 (5) ◽  
pp. 1297-1302 ◽  
Author(s):  
Pieter C. M. Res ◽  
Daniela L. M. Orsini ◽  
Jacob M. van Laar ◽  
Anneke A. M. Janson ◽  
Christiane Abou-Zeid ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Synovial Fluid ◽  
Antigen Recognition ◽  
T Cell Clones ◽  
Cell Clones
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