Low‐cost medium for 1,3‐propanediol production from crude glycerol by Clostridium butyricum

2020 ◽  
Vol 14 (5) ◽  
pp. 1125-1134
Author(s):  
Fernanda F Martins ◽  
Vanessa da S S Liberato ◽  
Cláudia Maria S Ribeiro ◽  
Maria Alice Z Coelho ◽  
Tatiana F Ferreira
Keyword(s):  
Crude Glycerol ◽  
Low Cost ◽  
Clostridium Butyricum

scholarly journals Waste Conversion into a Sweetener—Development of an Innovative Strategy for Erythritol Production by Yarrowia lipolytica

2020 ◽  
Vol 12 (17) ◽  
pp. 7122
Author(s):  
Ludwika Tomaszewska-Hetman ◽  
Waldemar Rymowicz ◽  
Anita Rywińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Culture Broth ◽  
Biodiesel Production ◽  
Innovative Strategy ◽  
Simple Medium ◽  
Low Cost Strategy ◽  
Steep Liquor

The study proposed the innovative low-cost strategy for erythritol production by Yarrowia lipolytica through developing a simple medium based on industrial waste by-products and a natural method for culture broth purification. Results obtained proved that corn steep liquor might successfully replace traditional sources of nitrogen and other nutrients without compromising activities of the enzymes responsible for erythritol production and its production level. As a consequence, a production process was performed where Y. lipolytica A-6 was able to produce 108.0 g/L of erythritol, with a production rate of 1.04 g/Lh and a yield of 0.45 g/g of the medium containing exclusively 220 g/L of crude glycerol derived from biodiesel production and 40 g/L of corn steep liquor. Moreover, a comparable concentration of erythritol (108.1 g/L) was obtained when a part of crude glycerol was exchanged for the crude fraction of fatty acids in the two-steps process. Next, the collected post-fermentation broths were used in the culture with Y. lipolytica Wratislavia K1 for natural purification. The process resulted in a high increase of erythritol selectivity from 72% to 97% and in the production of 22.0 g/L of biomass with 40.4% protein content, which enables its use as an attractive animal feedstuff.

High-level production of 1,3-propanediol from crude glycerol by Clostridium butyricum AKR102a

2011 ◽  
Vol 93 (3) ◽  
pp. 1057-1063 ◽  
Author(s):  
Erik Wilkens ◽  
Anne Katrin Ringel ◽  
Diana Hortig ◽  
Thomas Willke ◽  
Klaus-Dieter Vorlop
Keyword(s):  
Crude Glycerol ◽  
Clostridium Butyricum ◽  
High Level ◽  
Level Production

scholarly journals Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

2020 ◽  
Author(s):  
Feng Zhao ◽  
Chao Guo ◽  
Qingfeng Cui
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Practical Significance ◽  
Glycerol Metabolism ◽  
Metabolic Intermediate ◽  
Spectra Analysis ◽  
Low Cost Substrate ◽  
Anaerobic Production

Abstract Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

scholarly journals Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

2021 ◽  
Author(s):  
Feng Zhao ◽  
Mengyao Zheng ◽  
Qingfeng Cui
Keyword(s):  
Surface Tension ◽  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Practical Significance ◽  
Glycerol Metabolism ◽  
Metabolic Intermediate ◽  
Spectra Analysis ◽  
Low Cost Substrate ◽  
Anaerobic Production

Abstract Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

scholarly journals Glycerol or crude glycerol as substrates make Pseudomonas aeruginosa achieve anaerobic production of rhamnolipids

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Feng Zhao ◽  
Yuting Wu ◽  
Qingzhi Wang ◽  
Mengyao Zheng ◽  
Qingfeng Cui
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Glycerol ◽  
Low Cost ◽  
Carbon Sources ◽  
Anaerobic Growth ◽  
Strain Specificity ◽  
Spectra Analysis ◽  
Anoxic Environments ◽  
Anaerobic Production

Abstract Background The anaerobic production of rhamnolipids is significant in research and application, such as foamless fermentation and in situ production of rhamnolipids in the anoxic environments. Although a few studies reported that some rare Pseudomonas aeruginosa strains can produce rhamnolipids anaerobically, the decisive factors for anaerobic production of rhamnolipids were unknown. Results Two possible hypotheses on the decisive factors for anaerobic production of rhamnolipids by P. aeruginosa were proposed, the strains specificity of rare P. aeruginosa (hypothesis 1) and the effect of specific substrates (hypothesis 2). This study assessed the anaerobic growth and rhamnolipids synthesis of three P. aeruginosa strains using different substrates. P. aeruginosa strains anaerobically grew well using all the tested substrates, but glycerol was the only carbon source that supported anaerobic production of rhamnolipids. Other carbon sources with different concentrations still failed for anaerobic production of rhamnolipids by P. aeruginosa. Nitrate was the excellent nitrogen source for anaerobic production of rhamnolipids. FTIR spectra analysis confirmed the anaerobically produced rhamnolipids by P. aeruginosa using glycerol. The anaerobically produced rhamnolipids decreased air-water surface tension to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Crude glycerol and 1, 2-propylene glycol also supported the anaerobic production of rhamnolipids by all P. aeruginosa strains. Prospects and bottlenecks to anaerobic production of rhamnolipids were also discussed. Conclusions Glycerol substrate was the decisive factor for anaerobic production of rhamnolipids by P. aeruginosa. Strain specificity resulted in the different anaerobic yield of rhamnolipids. Crude glycerol was one low cost substrate for anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Results help advance the research on anaerobic production of rhamnolipids, deepen the biosynthesis theory of rhamnolipids and optimize the anaerobic production of rhamnolipids.

scholarly journals Fed-batch carotenoid production by Phaffia rhodozyma Y-17268 using agroindustrial substrates

2020 ◽  
Vol 10 (3) ◽  
pp. 5348-5354
Keyword(s):  
Crude Glycerol ◽  
Low Cost ◽  
Corn Steep Liquor ◽  
Aeration Rate ◽  
Phaffia Rhodozyma ◽  
Agitation Rate ◽  
Fed Batch ◽  
Batch Bioreactor ◽  
Steep Liquor ◽  
Β Carotene

This work aim the carotenoid bioproduction by the yeast Phaffia rhodozyma Y-17268 in a fed-batch bioreactor with different low-cost agroindustrial substrates (crude glycerol, corn steep liquor, and rice parboiling water). The maximum concentration of total carotenoid and cell productivity were 4118 µg/L (835 µg/g) and 0.05 g/L. h, respectively, with a feed volume of 75 mL every 12 h. The medium were composed of 100 g/L crude glycerol, 100 g/L corn steep liquor, and 20 g/L rice parboiling water at 25ºC, pHinitial 4.0, agitation rate of 250 rpm, aeration rate of 1.5 vvm and 96 h of bioproduction. 0.188 h-1 of maximum specific growth speeds (μmax) was obtained for the major carotenoid - (all-E)-β-carotene (75.9%). Thus, the yeast P. rhodozyma produced in a fed-batch bioreactor demonstrated a great potential to produce the β-carotene.

scholarly journals Effect of impurities in the crude glycerol polymerization reaction to produce polyglycerol

2019 ◽  
Vol 9 (2) ◽  
pp. 89-98
Author(s):  
Gustavo E. Ramirez Caballero ◽  
Carolina Ardila Suárez ◽  
Jeimmy Zulay Rojas Cristancho ◽  
Jennifer Paola Pineda Martínez ◽  
Alvaro Ramírez García
Keyword(s):  
Experimental Design ◽  
Crude Glycerol ◽  
Low Cost ◽  
Value Added ◽  
Polymerization Reaction ◽  
Reaction Products ◽  
Reaction Conditions ◽  
Raw Glycerol ◽  
Molecular Weights ◽  
Effect Of Impurities

Crude glycerol is a low-cost biodiesel industry co-product in a ratio of 1:10 with the main product. The large quantities of crude glycerol produced affect the biodiesel and glycerol markets. Consequently, the exploration of new applications for crude glycerol becomes crucial. As the composition of crude glycerol differs significantly from that of purified glycerol, this work was focused on studying the effect of crude glycerol impurities on its polymerization, as this process, at the same reaction conditions established for the polymerization of purified glycerol, did not result in polymeric products. To identify the reason for this result, the crude glycerol was fully characterized to identify its composition. Hence, an experimental design using simulated crude glycerol was performed to study the effect of the most abundant impurities in the raw glycerol on the polymerization andthe impurities that would persist with such reaction conditions, such as soap and sodium. The response variable for the experimental design was the hydroxyl number of the reaction products. FT-IR spectroscopy was used to analyze differences among the reaction products obtained from different treatments. The presence of soap was identified as primary inhibitory factor and the bottleneck in the formation of polyglycerol via polymerization of crude glycerol. Molecular weights of the polymerization reaction products were determined and analyzed as per the MALDI-TOF technique. The identification of the effect of impurities of crude glycerol polymerization suggests new routes for using it in the production of high value-added chemicals.

scholarly journals Reuse of polluting agroindustrial waste for ethanol production by Kluyveromyces marxianus

2019 ◽  
pp. 195-199 ◽  
Author(s):  
Joselma Ferreira da Silva ◽  
Naiara Priscila Silva Reis Barbosa ◽  
Matheus Tavares do Nascimento França ◽  
Laureen Michelle Houllou ◽  
Carolina Barbosa Malafaia
Keyword(s):  
Ethanol Production ◽  
Kluyveromyces Marxianus ◽  
Crude Glycerol ◽  
Cheese Whey ◽  
Low Cost ◽  
Dairy Industry ◽  
Alternative Source ◽  
Agroindustrial Residues ◽  
Agroindustrial Waste ◽  
Low Cost Substrate

The development of research for the production of biofuels using low cost substrate has become more relevant in recent years. These include reuse of residues such as crude residual glycerol from biodiesel (CRG) and cheese whey (CW) from the dairy industry. The present work evaluated the ethanol production by isolates of the yeast Kluyveromyces marxianus using agroindustrial residues as an alternative source of carbon. The cultures were rotated 100 rpm at 30 ° C for 24 h. The ethanol production was observed in both media, however, in the CW higher values of ethanol were observed in relation to the CRG. The results showed that K. marxianus isolates were adapted to the use of lactose present in cheese whey as a source of carbon for the production of ethanol with concentrations ranging from 11.41 to 19.9 g.L-1, but did not demonstrate efficiency in the use of crude glycerol for this purpose

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