Multi‐omics profiling of a CHO cell culture system unravels the effect of culture pH on cell growth, antibody titer and product quality

Chemically defined (CD) media are routinely used in the production of biologics in Chinese Hamster Ovary (CHO) cell culture and provide enhanced raw material control. Nutrient optimized CD media is an important path to increase cell growth and monoclonal antibody (mAb) productivity in recombinant CHO cell lines. However, nutrient optimization efforts for CD media typically rely on multi-factorial and experimental design of experiment (DoE) approaches or complex mathematical models of cellular metabolism or gene expression systems. Moreover, the majority of these efforts are aimed at amino acids since they constitute essential nutrients in CD media as they directly contribute to biomass and protein production. In this study, we demonstrate the utilization of multi-variate data analytics (MVDA) coupled with amino acid stoichiometric balances (SBs) to increased cell growth and mAb productivity in efforts to reduce CD media development efforts. SBs measure the difference between theoretical demand of amino acids and the empirically measured fluxes to identify metabolic states of the cell. When coupled with MVDA, the statistical models were not only able to highlight key amino acids towards cell growth or productivity, but also provided direction on metabolic favorability of the amino acid. Experimental validation of our approach resulted in a 55% increase in total cell growth and about an 80% increase in total mAb productivity. Increased specific consumption of stoichiometrically balanced amino acids and decreased specific consumption of glucose was also observed in optimized CD media suggesting favorable consumption of desired nutrients and a potential for energy redistribution towards increased cellular growth or mAb productivity.

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Study on Light Intensity Attenuation Characteristics of Chlorella Sp. Culture and the Growth of Chlorella Sp. with the Gradient Light Illuminate Culture

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.675-677.86 ◽

2014 ◽

Vol 675-677 ◽

pp. 86-89

Author(s):

Li Hong Yue ◽

Yi Peng Yang

Keyword(s):

Cell Culture ◽

Regression Analysis ◽

Cell Growth ◽

Light Intensity ◽

Culture System ◽

Culture Conditions ◽

Cell Culture System ◽

Intensity Attenuation ◽

Chlorella Sp ◽

Attenuation Characteristics

Based on the photoautotrophic characteristics of the freshwater microalage, light intensity attenuation characteristics in the cell culture system for Chlorella sp. were studied. Micralgae was cultured with a constant and the gradient light illuminate culture conditions, the cell growth of Microlagae was detected respectively, By means of regression analysis, mathematical models of illuminance intensity attenuation are gotten under different culture conditions. Results show algae cell density under gradient light culture conditions is higher than constant light, so the gradient light is more suitable for the cultivation of microalgae.

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Early Effects of Altered Gravity Environments on Plant Cell Growth and Cell Proliferation: Characterization of Morphofunctional Nucleolar Types in an Arabidopsis Cell Culture System

Frontiers in Astronomy and Space Sciences ◽

10.3389/fspas.2016.00002 ◽

2016 ◽

Vol 3 ◽

Cited By ~ 8

Author(s):

Ana I. Manzano ◽

Raúl Herranz ◽

Aránzazu Manzano ◽

Jack J. W. A. van Loon ◽

F. Javier Medina

Keyword(s):

Cell Culture ◽

Cell Proliferation ◽

Cell Growth ◽

Plant Cell ◽

Culture System ◽

Cell Culture System ◽

Altered Gravity ◽

Early Effects ◽

Plant Cell Growth

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Conversion of a CHO cell culture process from perfusion to fed-batch technology without altering product quality

Journal of Biotechnology ◽

10.1016/j.jbiotec.2005.10.013 ◽

2006 ◽

Vol 123 (1) ◽

pp. 106-116 ◽

Cited By ~ 25

Author(s):

F. Meuwly ◽

U. Weber ◽

T. Ziegler ◽

A. Gervais ◽

R. Mastrangeli ◽

...

Keyword(s):

Cell Culture ◽

Product Quality ◽

Cell Culture Process ◽

Fed Batch ◽

Cho Cell ◽

Culture Process ◽

Cho Cell Culture

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In vitro assessment of food-derived-glucose bioaccessibility and bioavailability in bicameral cell culture system

Turkish Journal of Biochemistry ◽

10.1515/tjb-2020-0082 ◽

2020 ◽

Vol 45 (5) ◽

pp. 631-637

Author(s):

Cansu Ozel-Tasci ◽

Gozde Pilatin ◽

Ozgur Edeer ◽

Sukru Gulec

Keyword(s):

Cell Culture ◽

Culture System ◽

Metabolic Diseases ◽

Enzymatic Digestion ◽

Cell Culture System ◽

Culture Studies ◽

Experimental Approaches ◽

In Vitro Assessment

AbstractBackgroundFunctional foods can help prevent metabolic diseases, and it is essential to evaluate functional characteristics of foods through in vitro and in vivo experimental approaches.ObjectiveWe aimed to use the bicameral cell culture system combined with the in vitro digestion to evaluate glucose bioavailability.Materials and methodsCake, almond paste, and pudding were modified by adding fiber and replacing sugar with sweeteners and polyols. Digestion process was modeled in test tubes. Rat enterocyte cells (IEC-6) were grown in a bicameral cell culture system to mimic the physiological characteristics of the human intestine. The glucose bioaccessibility and cellular glucose efflux were measured by glucose oxidase assay.Results and discussionThe glucose bioaccessibilities of modified foods were significantly lower (cake: 2.6 fold, almond paste: 9.2 fold, pudding 2.8 fold) than the controls. Cellular glucose effluxes also decreased in the modified cake, almond paste, and pudding by 2.2, 4, and 2 fold respectively compared to their controls.ConclusionOur results suggest that combining in vitro enzymatic digestion with cell culture studies can be a practical way to test in vitro glucose bioaccessibility and bioavailability in functional food development.

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