A rapid analytical technique for the determination of energy expenditure by the doubly labelled water method

Introduction. Proper nutrition is crucial for child and adolescent athletes to maintain growth and development and to achieveoptimal results in sports. It is very important to balance the energy expenditure with the energy intake in order to prevent the energy deficit or excess.Materials and methods. Subjects involved in two different sports participated in the study: 13 gymnasts (age 13.8 ± 4.1 years, height 153.4 ± 11.3 cm, weight 47.1 ± 10.5 kg) and 15 basketball players (age 15.5 ± 1.1 years, height 176.7 ± 7.9 cm, weight 65.2 ± 10.7 kg). Determination of total energy expenditure was made by prediction equations. The subjects maintained a food records for 5 consecutive days, which were processed in the ASA24 system of the NCI. Results and discussion. Energy intake in both groups is sufficient to meet the daily needs, development of young athletes andprovide the energy needed in training. The intake of three minerals (calcium, magnesium and potassium) and three vitamins (D, E and A) was lower than recommended values in both groups.Conclusion. As a result of the busy schedule of adolescent athletes, their main meals are out of home, and the proportion of highly processed foods containing small amounts of important vitamins and minerals is high. The main recommendations include dairy products, fruits, vegetables and whole grains. The idea behind the changes is to give young athletes the right diet and the right eating habits.

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Advances in the Chromatographic Separation and Determination of Bioactive Compounds for Assessing the Nutrient Profile of Nuts

Current Analytical Chemistry ◽

10.2174/1573411016999200729111951 ◽

2020 ◽

Vol 16 ◽

Author(s):

Natasa P. Kalogiouri ◽

Natalia Manousi ◽

Erwin Rosenberg ◽

George A. Zachariadis ◽

Victoria F. Samanidou

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

High Performance ◽

Unsaturated Fatty Acids ◽

Nutritional Deficiencies ◽

Analytical Technique ◽

High Volatility ◽

Analytical Approaches

Background:: Nuts have been incorporated into guidelines for healthy eating since they contain considerable amounts of antioxidants and their effects are related to health benefits since they contribute to the prevention of nutritional deficiencies. The micronutrient characterization is based mainly on the determination of phenolics which is the most abundant class of bioactive compounds in nuts. Terpenes constitute another class of bioactive compounds that are present in nuts and show high volatility. The analysis of phenolic compounds and terpenes are very demanding tasks that require optimization of the chromatographic conditions to improve the separation of the components. Moreover, nuts are rich in unsaturated fatty acids and they are therefore considered as cardioprotective. Gas chromatography is the predominant instrumental analytical technique for the determination of derivatized fatty acids and terpenes in food matrices, while high performance liquid chromatography is currently the most popular technique for the determination of phenolic compounds Objective:: This review summarizes all the recent advances in the optimization of the chromatographic conditions for the determination of phenolic compounds, fatty acids and terpenes in nuts Conclusion:: The state-of-the art in the technology available is critically discussed, exploring new analytical approaches to reduce the time of analysis and improve the performance of the chromatographic systems in terms of precision, reproducibility, limits of detection and quantification and overall quality of the results

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A new doubly labelled water anthropometry-based equation for prediction of total daily energy expenditure in older people from low- and middle-income countries

European Journal of Clinical Nutrition ◽

10.1038/s41430-021-00886-6 ◽

2021 ◽

Author(s):

Heliodoro Alemán-Mateo ◽

Lesley Evelyn Antúnez-Roman ◽

Julián Esparza-Romero ◽

Mauro E. Valencia ◽

Julissa J. Salguero ◽

...

Keyword(s):

Energy Expenditure ◽

Older People ◽

Daily Energy Expenditure ◽

Doubly Labelled Water ◽

Middle Income ◽

Middle Income Countries ◽

Daily Energy ◽

Total Daily Energy Expenditure ◽

Low And Middle Income

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The Past, Present and Future of Flow Cytometry in Central Nervous System Malignancies

Methods and Protocols ◽

10.3390/mps4010011 ◽

2021 ◽

Vol 4 (1) ◽

pp. 11

Author(s):

Evrysthenis Vartholomatos ◽

George Vartholomatos ◽

George A. Alexiou ◽

Georgios S. Markopoulos

Keyword(s):

Central Nervous System ◽

Flow Cytometry ◽

Nervous System ◽

Therapeutic Agents ◽

Phenotypic Characterization ◽

Cell Phenotype ◽

Analytical Technique ◽

The Past ◽

Current Review

Central nervous system malignancies (CNSMs) are categorized among the most aggressive and deadly types of cancer. The low median survival in patients with CNSMs is partly explained by the objective difficulties of brain surgeries as well as by the acquired chemoresistance of CNSM cells. Flow Cytometry is an analytical technique with the ability to quantify cell phenotype and to categorize cell populations on the basis of their characteristics. In the current review, we summarize the Flow Cytometry methodologies that have been used to study different phenotypic aspects of CNSMs. These include DNA content analysis for the determination of malignancy status and phenotypic characterization, as well as the methodologies used during the development of novel therapeutic agents. We conclude with the historical and current utility of Flow Cytometry in the field, and we propose how we can exploit current and possible future methodologies in the battle against this dreadful type of malignancy.

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