Shift to high‐intensity, low‐volume perfusion cell culture enabling a continuous, integrated bioprocess

2018 ◽  
Vol 34 (6) ◽  
pp. 1472-1481 ◽  
Author(s):  
Matthew Gagnon ◽  
Shashikant Nagre ◽  
Wenge Wang ◽  
Gregory W. Hiller
Keyword(s):  
Cell Culture ◽  
High Intensity ◽  
Perfusion Cell Culture ◽  
Low Volume ◽  
Integrated Bioprocess

Anti-apoptotic genes Aven and E1B-19K enhance performance of BHK cells engineered to express recombinant factor VIII in batch and low perfusion cell culture

2007 ◽  
Vol 98 (4) ◽  
pp. 825-841 ◽  
Author(s):  
Toey Nivitchanyong ◽  
Amanda Martinez ◽  
Adiba Ishaque ◽  
John E. Murphy ◽  
Konstantin Konstantinov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Factor Viii ◽  
Recombinant Factor Viii ◽  
Perfusion Cell Culture ◽  
Bhk Cells ◽  
Apoptotic Genes

Is high-intensity interval training a time-efficient exercise strategy to improve health and fitness?

2014 ◽  
Vol 39 (3) ◽  
pp. 409-412 ◽  
Author(s):  
Jenna B. Gillen ◽  
Martin J. Gibala
Keyword(s):  
High Intensity ◽  
Disease Risk ◽  
Interval Training ◽  
High Volume ◽  
Oxidative Capacity ◽  
High Intensity Interval Training ◽  
Exercise Participation ◽  
Cardiometabolic Disorders ◽  
Low Volume ◽  
High Intensity Interval

Growing research suggests that high-intensity interval training (HIIT) is a time-efficient exercise strategy to improve cardiorespiratory and metabolic health. “All out” HIIT models such as Wingate-type exercise are particularly effective, but this type of training may not be safe, tolerable or practical for many individuals. Recent studies, however, have revealed the potential for other models of HIIT, which may be more feasible but are still time-efficient, to stimulate adaptations similar to more demanding low-volume HIIT models and high-volume endurance-type training. As little as 3 HIIT sessions per week, involving ≤10 min of intense exercise within a time commitment of ≤30 min per session, including warm-up, recovery between intervals and cool down, has been shown to improve aerobic capacity, skeletal muscle oxidative capacity, exercise tolerance and markers of disease risk after only a few weeks in both healthy individuals and people with cardiometabolic disorders. Additional research is warranted, as studies conducted have been relatively short-term, with a limited number of measurements performed on small groups of subjects. However, given that “lack of time” remains one of the most commonly cited barriers to regular exercise participation, low-volume HIIT is a time-efficient exercise strategy that warrants consideration by health practitioners and fitness professionals.

A Single Session of Low-Volume High-Intensity Interval Exercise Reduces Ambulatory Blood Pressure in Normotensive Men

2017 ◽  
Vol 31 (8) ◽  
pp. 2263-2269 ◽  
Author(s):  
Teresa C.B. Dantas ◽  
Luiz F. Farias Junior ◽  
Danniel T. Frazão ◽  
Paulo H.M. Silva ◽  
Altieres E. Sousa Junior ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Ambulatory Blood Pressure ◽  
High Intensity ◽  
Single Session ◽  
Interval Exercise ◽  
Low Volume ◽  
High Intensity Interval

scholarly journals The Effect of Low-Volume Sprint Interval Training on the Development and Subsequent Maintenance of Aerobic Fitness in Soccer Players

2015 ◽  
Vol 10 (3) ◽  
pp. 332-338 ◽  
Author(s):  
Tom W. Macpherson ◽  
Matthew Weston
Keyword(s):  
Beneficial Effect ◽  
Aerobic Fitness ◽  
High Intensity ◽  
Aerobic Training ◽  
Interval Training ◽  
Soccer Players ◽  
Control Group ◽  
Sprint Interval Training ◽  
Low Volume ◽  
Test Level

Purpose:To examine the effect of low-volume sprint interval training (SIT) on the development (part 1) and subsequent maintenance (part 2) of aerobic fitness in soccer players.Methods:In part 1, 23 players from the same semiprofessional team participated in a 2-wk SIT intervention (SIT, n = 14, age 25 ± 4 y, weight 77 ± 8 kg; control, n = 9, age 27 ± 6 y, weight 72 ± 10 kg). The SIT group performed 6 training sessions of 4–6 maximal 30-s sprints, in replacement of regular aerobic training. The control group continued with their regular training. After this 2-wk intervention, the SIT group was allocated to either intervention (n = 7, 1 SIT session/wk as replacement of regular aerobic training) or control (n = 7, regular aerobic training with no SIT sessions) for a 5-wk period (part 2). Pre and post measures were the YoYo Intermittent Recovery Test Level 1 (YYIRL1) and maximal oxygen uptake (VO2max).Results:In part 1, the 2-week SIT intervention had a small beneficial effect on YYIRL1 (17%; 90% confidence limits ±11%), and VO2max (3.1%; ±5.0%) compared with control. In part 2, 1 SIT session/wk for 5 wk had a small beneficial effect on VO2max (4.2%; ±3.0%), with an unclear effect on YYIRL1 (8%; ±16%).Conclusion:Two weeks of SIT elicits small improvements in soccer players’ high-intensity intermittent-running performance and VO2max, therefore representing a worthwhile replacement of regular aerobic training. The effectiveness of SIT for maintaining SIT-induced improvements in high-intensity intermittent running requires further research.

Effect of Low-Volume High-Intensity Interval Exercise and Continuous Exercise on Delayed-Onset Muscle Soreness in Untrained Healthy Males

2019 ◽  
Vol 33 (3) ◽  
pp. 774-782 ◽  
Author(s):  
Luiz F. Farias Junior ◽  
Rodrigo A.V. Browne ◽  
Danniel T. Frazão ◽  
Teresa C.B. Dantas ◽  
Paulo H.M. Silva ◽  
...  
Keyword(s):  
High Intensity ◽  
Muscle Soreness ◽  
Delayed Onset Muscle Soreness ◽  
Interval Exercise ◽  
Continuous Exercise ◽  
Delayed Onset ◽  
Low Volume ◽  
High Intensity Interval ◽  
Healthy Males

scholarly journals Low-volume high-intensity interval training improves cardiometabolic health, work ability and well-being in severely obese individuals: a randomized-controlled trial sub-study

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Dejan Reljic ◽  
Fabienne Frenk ◽  
Hans J. Herrmann ◽  
Markus F. Neurath ◽  
Yurdagül Zopf
Keyword(s):  
Weight Loss ◽  
Work Ability ◽  
High Intensity ◽  
Interval Training ◽  
Well Being ◽  
Cardiometabolic Health ◽  
High Intensity Interval Training ◽  
Severely Obese ◽  
Low Volume ◽  
High Intensity Interval

Abstract Background Obesity is associated with impaired health and lower work ability. Increased physical activity is a cornerstone in the treatment of obesity and related risk factors. Recently, high-intensity interval training (HIIT) has emerged as a popular exercise option. However, data regarding the effects on cardiometabolic health, perceived work ability and well-being in severely obese individuals are lacking. Methods Sixty-five obese individuals with sedentary occupation (48.7 ± 9.9 years, BMI: 39.6 ± 7.1 kg/m2) were randomly allocated to an extremely time-efficient HIIT (5 × 1 min at 80–95% maximal heart rate on cycle ergometers, 2×/week for 12 weeks) or an inactive control group (CON). Both groups received nutritional counseling to support weight loss. Primary outcome was maximal oxygen uptake (VO2max), secondary outcomes were cardiometabolic risk indices, body composition, work ability index (WAI), quality of life (QoL, EQ-5D-5L-questionnaire) and perceived stress (PSQ-questionnaire). Results Mean body weight reduction was 5.3 kg [95% confidence interval (95% CI) − 7.3 to − 3.3 kg] in the HIIT group (P < 0.001) and 3.7 kg (95% CI − 5.3 to − 2.1 kg) in CON (P < 0.001), respectively. Only the HIIT group showed significant (P < 0.001) changes in VO2max [+ 3.5 mL/kg/min (95% CI 2.5 to 4.6 mL/kg/min)], waist circumference [–7.5 cm (95% CI − 9.8 to − 5.1 kg)], mean arterial blood pressure [− 11 mmHg (95% CI − 14 to − 8 mmHg)], WAI [+ 3.0 points (95% CI 1.7 to 4.3 points)] and QoL [+ 10% (95% CI 5 to 16%)]. In CON, none of these parameters improved significantly. Conclusions Low-volume HIIT may induce significant improvements in cardiometabolic health, especially VO2max, WAI and well-being in obese individuals after only 12 weeks. Our results underpin the wide range of benefits on health and subjective measures through exercise that go well beyond simple weight loss through dietary restriction alone. Trial registration: ClinicalTrials.gov Id: NCT03306069. Registered 10 October 2017, https://clinicaltrials.gov/ct2/show/NCT03306069.

Chemically Induced Cell Cycle Arrest in Perfusion Cell Culture

2016 ◽  
pp. 161-176 ◽  
Author(s):  
Gabor Nagy ◽  
Bence Tanczos ◽  
Eszter Fidrus ◽  
Laszlo Talas ◽  
Gaspar Banfalvi
Keyword(s):  
Cell Cycle ◽  
Cell Culture ◽  
Cell Cycle Arrest ◽  
Perfusion Cell Culture ◽  
Cycle Arrest ◽  
Chemically Induced

Sex Specific Adaptations To Very Low-volume High-intensity Interval Training In Overweight Men And Women

2014 ◽  
Vol 46 ◽  
pp. 721
Author(s):  
Jenna B. Gillen ◽  
Lauren E. Skelly ◽  
Rachel B. Tan ◽  
Brian Martin ◽  
Michael E. Percival ◽  
...  
Keyword(s):  
High Intensity ◽  
Interval Training ◽  
High Intensity Interval Training ◽  
Men And Women ◽  
Low Volume ◽  
High Intensity Interval
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