Three‐dimensional cancer cell culture in high‐yield multiscale scaffolds by shear spinning

2018 ◽  
Vol 35 (2) ◽  
pp. e2750 ◽  
Author(s):  
Ahmed A. Ahmed ◽  
CJ Luo ◽  
Sandra Perez‐Garrido ◽  
Connor R. Browse ◽  
Christopher Thrasivoulou ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cell ◽  
Three Dimensional ◽  
High Yield ◽  
Shear Spinning

Gelatine methacrylamide-based hydrogels: An alternative three-dimensional cancer cell culture system

2014 ◽  
Vol 10 (6) ◽  
pp. 2551-2562 ◽  
Author(s):  
Elke Kaemmerer ◽  
Ferry P.W. Melchels ◽  
Boris M. Holzapfel ◽  
Tobias Meckel ◽  
Dietmar W. Hutmacher ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cancer Cell ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Culture System

scholarly journals Detection of Atomic Scale Changes in the Free Volume Void Size of Three-Dimensional Colorectal Cancer Cell Culture Using Positron Annihilation Lifetime Spectroscopy

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e83838 ◽  
Author(s):  
Eneko Axpe ◽  
Tamara Lopez-Euba ◽  
Ainara Castellanos-Rubio ◽  
David Merida ◽  
Jose Angel Garcia ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Culture ◽  
Positron Annihilation ◽  
Free Volume ◽  
Cancer Cell ◽  
Three Dimensional ◽  
Positron Annihilation Lifetime Spectroscopy ◽  
Atomic Scale ◽  
Void Size ◽  
Positron Annihilation Lifetime

Abstract 5437: Novel drug sensitivity test using three-dimensional primary lung cancer cell culture on microfabricated surface

2011 ◽  
Author(s):  
Manami Shimomura ◽  
Ruriko Sakamoto ◽  
Kaori Yoshida ◽  
Ikuyo Ueda ◽  
Masahito Yonemura ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Drug Sensitivity ◽  
Three Dimensional ◽  
Primary Lung Cancer ◽  
Sensitivity Test ◽  
Lung Cancer Cell ◽  
Drug Sensitivity Test ◽  
Novel Drug

scholarly journals A simple, cost-effective method for generating murine colonic 3D enteroids and 2D monolayers for studies of primary epithelial cell function

2017 ◽  
Vol 313 (5) ◽  
pp. G467-G475 ◽  
Author(s):  
Elizabeth H. Fernando ◽  
Michael Dicay ◽  
Martin Stahl ◽  
Marilyn H. Gordon ◽  
Andrew Vegso ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Three Dimensional ◽  
Cost Effective ◽  
Cancer Cell Lines ◽  
High Yield ◽  
Intestinal Epithelial ◽  
Primary Cells ◽  
Epithelial Monolayers

Cancer cell lines have been the mainstay of intestinal epithelial experimentation for decades, due primarily to their immortality and ease of culture. However, because of the inherent biological abnormalities of cancer cell lines, many cellular biologists are currently transitioning away from these models and toward more representative primary cells. This has been particularly challenging, but recent advances in the generation of intestinal organoids have brought the routine use of primary cells within reach of most epithelial biologists. Nevertheless, even with the proliferation of publications that use primary intestinal epithelial cells, there is still a considerable amount of trial and error required for laboratories to establish a consistent and reliable method to culture three-dimensional (3D) intestinal organoids and primary epithelial monolayers. We aim to minimize the time other laboratories spend troubleshooting the technique and present a standard method for culturing primary epithelial cells. Therefore, we have described our optimized, high-yield, cost-effective protocol to grow 3D murine colonoids for more than 20 passages and our detailed methods to culture these cells as confluent monolayers for at least 14 days, enabling a wide variety of potential future experiments. By supporting and expanding on the current literature of primary epithelial culture optimization and detailed use in experiments, we hope to help enable the widespread adoption of these innovative methods and allow consistency of results obtained across laboratories and institutions. NEW & NOTEWORTHY Primary intestinal epithelial monolayers are notoriously difficult to maintain culture, even with the recent advances in the field. We describe, in detail, the protocols required to maintain three-dimensional cultures of murine colonoids and passage these primary epithelial cells to confluent monolayers in a standardized, high-yield and cost-effective manner.

scholarly journals Electrospinning PCL Scaffolds Manufacture for Three-Dimensional Breast Cancer Cell Culture

Polymers ◽  
2017 ◽  
Vol 9 (12) ◽  
pp. 328 ◽  
Author(s):  
Marc Rabionet ◽  
Marc Yeste ◽  
Teresa Puig ◽  
Joaquim Ciurana
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Breast Cancer Cell Culture

scholarly journals PEG-fibrinogen hydrogels for three-dimensional breast cancer cell culture

2016 ◽  
Vol 105 (1) ◽  
pp. 236-252 ◽  
Author(s):  
Shantanu Pradhan ◽  
Iman Hassani ◽  
Wen J. Seeto ◽  
Elizabeth A. Lipke
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Breast Cancer Cell Culture

scholarly journals A dataset on the effect of exercise-conditioned human sera in three-dimensional breast cancer cell culture

Data in Brief ◽  
2019 ◽  
Vol 27 ◽  
pp. 104704 ◽  
Author(s):  
Mauro De Santi ◽  
Giulia Baldelli ◽  
Francesco Lucertini ◽  
Valentina Natalucci ◽  
Giorgio Brandi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Culture ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Three Dimensional ◽  
Human Sera ◽  
Breast Cancer Cell Culture

Creation of macropores in three-dimensional bacterial cellulose scaffold for potential cancer cell culture

2014 ◽  
Vol 114 ◽  
pp. 553-557 ◽  
Author(s):  
Guangyao Xiong ◽  
Honglin Luo ◽  
Yong Zhu ◽  
Sudha Raman ◽  
Yizao Wan
Keyword(s):  
Cell Culture ◽  
Bacterial Cellulose ◽  
Cancer Cell ◽  
Three Dimensional ◽  
Potential Cancer
Sign in / Sign up

Export Citation Format

Share Document