Effects of hyperthermia on heat shock protein expression, alkaline phosphatase activity and proliferation in human osteosarcoma cells

2007 ◽  
Vol 25 (6) ◽  
pp. 669-672 ◽  
Author(s):  
K. Trieb ◽  
H. Blahovec ◽  
B. Kubista
Keyword(s):  
Alkaline Phosphatase ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Protein Expression ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells ◽  
Heat Shock Protein Expression

scholarly journals Presence and activity of alkaline phosphatase in two human osteosarcoma cell lines.

1989 ◽  
Vol 37 (7) ◽  
pp. 1069-1074 ◽  
Author(s):  
J C Randall ◽  
D C Morris ◽  
S Zeiger ◽  
K Masuhara ◽  
T Tsuda ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Cell Membrane ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Osteogenic Sarcoma ◽  
Ultrastructural Level ◽  
Osteosarcoma Cell ◽  
Osteosarcoma Cells ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells

The presence and activity of alkaline phosphatase in SAOS-2 and TE-85 human osteosarcoma cells grown in culture were examined at the ultrastructural level. A monoclonal antibody raised against purified human bone osteosarcoma alkaline phosphatase was used to localize the enzyme in cultures of the osteosarcoma cells. Similar cultures were analyzed for alkaline phosphatase activity using an enzyme cytochemical method with cerium as the capture agent. Alkaline phosphatase was immunolocalized at the light microscopic level in an osteogenic sarcoma and ultrastructurally on the SAOS-2 cell membrane and the enclosing membrane of extracellular vesicular structures close to the cells. In contrast, the TE-85 cells were characterized by the absence of all but a few traces of immunolabeling at the cell surface. Enzyme cytochemical studies revealed strong alkaline phosphatase activity on the outer surface of the SAOS-2 cell membrane. Much lower enzyme activity was observed in the TE-85 cells. The results support biochemical data from previous studies and confirm that SAOS-2 cells have a significantly greater concentration of alkaline phosphatase at the plasma membrane.

Heat stress induces alkaline phosphatase activity and heat shock protein 25 expression in cultured pulp cells

2007 ◽  
Vol 0 (0) ◽  
pp. 071204000232001-??? ◽  
Author(s):  
M.-W. Lee ◽  
T. Muramatsu ◽  
T. Uekusa ◽  
J.-H. Lee ◽  
M. Shimono
Keyword(s):  
Alkaline Phosphatase ◽  
Heat Stress ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Pulp Cells

scholarly journals Suppression of heat shock protein 70 by siRNA enhances the antitumor effects of cisplatin in cultured human osteosarcoma cells

2017 ◽  
Vol 22 (5) ◽  
pp. 699-706 ◽  
Author(s):  
Yuki Mori ◽  
Ryu Terauchi ◽  
Toshiharu Shirai ◽  
Shinji Tsuchida ◽  
Naoki Mizoshiri ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Osteosarcoma Cells ◽  
Antitumor Effects ◽  
Human Osteosarcoma ◽  
Human Osteosarcoma Cells
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