Ratiometric Detection of Glutathione Based on Disulfide Linkage Rupture between a FRET Coumarin Donor and a Rhodamine Acceptor

ChemBioChem ◽  
2021 ◽  
Author(s):  
Yibin Zhang ◽  
Shuai Xia ◽  
Shulin Wan ◽  
Tessa E. Steenwinkel ◽  
Tara Vohs ◽  
...  
2021 ◽  
Vol 1147 ◽  
pp. 170-177
Author(s):  
Pingping Ji ◽  
Guimei Han ◽  
Yan Huang ◽  
Hongxin Jiang ◽  
Qiwen Zhou ◽  
...  

2021 ◽  
Author(s):  
Shilpi Jaiswal ◽  
Subhankar Kundu ◽  
Sujoy Bandyopadhyay ◽  
Abhijit Patra

An organic–inorganic hybrid upconversion nanoprobe was developed for the real-time detection of aliphatic biogenic amines in an aqueous medium, adulterated milk, and rotten fish.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Anna Kanegae ◽  
Yusuke Takata ◽  
Ippei Takashima ◽  
Shohei Uchinomiya ◽  
Ryosuke Kawagoe ◽  
...  

AbstractDespite continuous and active development of fluorescent metal-ion probes, their molecular design for ratiometric detection is restricted by the limited choice of available sensing mechanisms. Here we present a multicolor and ratiometric fluorescent sensing platform for metal ions based on the interaction between the metal ion and the aromatic ring of a fluorophore (arene–metal-ion, AM, coordination). Our molecular design provided the probes possessing a 1,9-bis(2′-pyridyl)-2,5,8-triazanonane as a flexible metal ion binding unit attached to a tricyclic fluorophore. This architecture allows to sense various metal ions, such as Zn(II), Cu(II), Cd(II), Ag(I), and Hg(II) with emission red-shifts. We showed that this probe design is applicable to a series of tricyclic fluorophores, which allow ratiometric detection of the metal ions from the blue to the near-infrared wavelengths. X-ray crystallography and theoretical calculations indicate that the coordinated metal ion has van der Waals contact with the fluorophore, perturbing the dye’s electronic structure and ring conformation to induce the emission red-shift. A set of the probes was useful for the differential sensing of eight metal ions in a one-pot single titration via principal component analysis. We also demonstrate that a xanthene fluorophore is applicable to the ratiometric imaging of metal ions under live-cell conditions.


2019 ◽  
Vol 43 (7) ◽  
pp. 2875-2877 ◽  
Author(s):  
Luling Wu ◽  
Liyuan Liu ◽  
Hai-Hao Han ◽  
Xue Tian ◽  
Maria L. Odyniec ◽  
...  

A simple ESIPT-based fluorescence probe (HMBT-LW) was developed for the detection of superoxide (O2˙−).


2019 ◽  
Vol 6 (4) ◽  
pp. 1121-1130 ◽  
Author(s):  
Francisco Yarur ◽  
Jun-Ray Macairan ◽  
Rafik Naccache

Classical methods for the detection of heavy metal ions in water are tedious and time consuming.


2018 ◽  
Vol 265 ◽  
pp. 365-370 ◽  
Author(s):  
Yuliang Jiang ◽  
Shishan Wu ◽  
Can Jin ◽  
Bingxiang Wang ◽  
Jian Shen

1984 ◽  
Vol 159 (5) ◽  
pp. 1351-1370 ◽  
Author(s):  
G K Schoolnik ◽  
R Fernandez ◽  
J Y Tai ◽  
J Rothbard ◽  
E C Gotschlich

The complete amino acid sequence of pilin from gonococcal strain MS11 and the sequence of constant and variable regions from strain R10 pilin have been determined in order to elucidate the structural basis for adherence function, antigenic diversity, and polymeric structure. The MS11 pilin sequence consists of 159 amino acids in a single polypeptide chain with two cysteines in disulfide linkage and serine-bonded phosphate residues. TC-2 (31-111), a soluble monomeric pilus peptide prepared by arginine-specific digestion, bound human endocervical, but not buccal or HeLa cells and therefore is postulated to encompass the receptor binding domain. Variable regions of CNBr-3 appear to confer antigenic diversity and comprise segments in which changes in the position of charged residues occur in hydrophilic, beta-turns. Residues 2-21 and 202-221 of gonococcal pilins and lower eucaryotic actins, respectively, exhibit 50% homology. When these residues are arranged at intervals of 100 degrees of arc on "helical wheels," the identical amino acids comprise a hydrophobic face on one side of the helix. This observation, the hydrophobic character of this region and the tendency for TC-1 (residues 1-30) to aggregate in water, suggest that this stretch interacts with other subunits to stabilize polymeric structure.


2017 ◽  
Vol 28 (6) ◽  
pp. 1099-1108 ◽  
Author(s):  
Kirt L. Durand ◽  
Lei Tan ◽  
Craig A. Stinson ◽  
Chasity B. Love-Nkansah ◽  
Xiaoxiao Ma ◽  
...  

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