ChemInform Abstract: KINETIC PARAMETERS FOR A SYSTEM AT EQUILIBRIUM FROM THE TIME COURSE OF LUMINESCENCE EMISSION: A NEW PROBE OF EQUILIBRIUM DYNAMICS. EXCITED-STATE EUROPIUM(III) AS A SPECIES LABEL

1983 ◽  
Vol 14 (36) ◽  
Author(s):  
W. DEW. JUN. HORROCKS ◽  
V. K. ARKLE ◽  
F. J. LIOTTA ◽  
D. R. SUDNICK
Keyword(s):  
Excited State ◽  
Kinetic Parameters ◽  
Time Course ◽  
Equilibrium Dynamics ◽  
Luminescence Emission

scholarly journals Contribution of the intra- and intermolecular routes in autocatalytic zymogen activation: application to pepsinogen activation.

2006 ◽  
Vol 53 (2) ◽  
pp. 407-420 ◽  
Author(s):  
Ramón Varón ◽  
Matilde E Fuentes ◽  
Manuela García-Moreno ◽  
Francisco Garcìa-Sevilla ◽  
Enrique Arias ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Rate Constants ◽  
Time Course ◽  
Initial Conditions ◽  
Reaction Scheme ◽  
Complete Analysis ◽  
Zymogen Activation ◽  
Relative Contribution ◽  
Starting Point ◽  
Definition Of

Taking as the starting point a recently suggested reaction scheme for zymogen activation involving intra- and intermolecular routes and the enzyme-zymogen complex, we carry out a complete analysis of the relative contribution of both routes in the process. This analysis suggests the definition of new dimensionless parameters allowing the elaboration, from the values of the rate constants and initial conditions, of the time course of the contribution of the two routes. The procedure mentioned above related to a concrete reaction scheme is extrapolated to any other model of autocatalytic zymogen activation involving intra- and intermolecular routes. Finally, we discuss the contribution of both of the activating routes in pepsinogen activation into pepsin using the values of the kinetic parameters given in the literature.

scholarly journals Convenient Method for Estimation of Kinetic Parameters from Time Course Analysis ofEnzyme Reactions witha Microcomputer

1984 ◽  
Vol 48 (2) ◽  
pp. 533-536
Author(s):  
Tadashi Yoshimoto ◽  
Futoshi Matsubara ◽  
Daisuke Tsuru ◽  
Juichiro Shibasaki
Keyword(s):  
Kinetic Parameters ◽  
Convenient Method ◽  
Time Course

scholarly journals Damming a Molecular Energy Reservoir: Ion-regulated Electronic Energy Shuttling in a [2]Rotaxane

2021 ◽  
Author(s):  
Shilin Yu ◽  
Arkady Kurpryakov ◽  
James Lewis ◽  
Vicente Martí-Centelles ◽  
Stephen Goldup ◽  
...  
Keyword(s):  
Excited State ◽  
Kinetic Parameters ◽  
State Energy ◽  
Energy Levels ◽  
Electronic Energy ◽  
Cation Binding ◽  
Potential Mechanism ◽  
Delayed Luminescence ◽  
Excited State Energy

Bidirectional electronic energy shuttling is shown to occur between the molecular ring and axle components of a rotaxane. The engineered energetic and kinetic parameters give rise to long-lived, delayed luminescence. Perturbation of the quasi-isoenergetic ring and stopper chromophore excited-state energy levels upon cation binding influences the energy shuttling process, and hence luminescence read-out, representing a new potential mechanism in luminescent molecular chemosensor development

scholarly journals A minimal model for gene expression dynamics of bacterial type II toxin–antitoxin systems

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kosmas Kosmidis ◽  
Marc-Thorsten Hütt
Keyword(s):  
Gene Expression ◽  
Mathematical Modelling ◽  
Kinetic Parameters ◽  
Stress Responses ◽  
Minimal Model ◽  
Time Course ◽  
Mechanistic Model ◽  
Quantitative Description ◽  
Type Ii ◽  
Computational Systems Biology

AbstractToxin–antitoxin (TA) modules are part of most bacteria’s regulatory machinery for stress responses and general aspects of their physiology. Due to the interplay of a long-lived toxin with a short-lived antitoxin, TA modules have also become systems of interest for mathematical modelling. Here we resort to previous modelling efforts and extract from these a minimal model of type II TA system dynamics on a timescale of hours, which can be used to describe time courses derived from gene expression data of TA pairs. We show that this model provides a good quantitative description of TA dynamics for the 11 TA pairs under investigation here, while simpler models do not. Our study brings together aspects of Biophysics with its focus on mathematical modelling and Computational Systems Biology with its focus on the quantitative interpretation of ’omics’ data. This mechanistic model serves as a generic transformation of time course information into kinetic parameters. The resulting parameter vector can, in turn, be mechanistically interpreted. We expect that TA pairs with similar mechanisms are characterized by similar vectors of kinetic parameters, allowing us to hypothesize on the mode of action for TA pairs still under discussion.

scholarly journals Damming a Molecular Energy Reservoir: Ion-regulated Electronic Energy Shuttling in a [2]Rotaxane

2021 ◽  
Author(s):  
Shilin Yu ◽  
Arkady Kurpryakov ◽  
James Lewis ◽  
Vicente Martí-Centelles ◽  
Stephen Goldup ◽  
...  
Keyword(s):  
Excited State ◽  
Kinetic Parameters ◽  
State Energy ◽  
Energy Levels ◽  
Electronic Energy ◽  
Cation Binding ◽  
Potential Mechanism ◽  
Delayed Luminescence ◽  
Excited State Energy

Bidirectional electronic energy shuttling is shown to occur between the molecular ring and axle components of a rotaxane. The engineered energetic and kinetic parameters give rise to long-lived, delayed luminescence. Perturbation of the quasi-isoenergetic ring and stopper chromophore excited-state energy levels upon cation binding influences the energy shuttling process, and hence luminescence read-out, representing a new potential mechanism in luminescent molecular chemosensor development

Similarity between the kinetic parameters of the buffer-mediated proton exchange reaction of a xanthenic derivative in its ground- and excited-state

2010 ◽  
Vol 12 (2) ◽  
pp. 323-327 ◽  
Author(s):  
Jose M. Paredes ◽  
Angel Orte ◽  
Luis Crovetto ◽  
Jose M. Alvarez-Pez ◽  
Ramon Rios ◽  
...  
Keyword(s):  
Excited State ◽  
Kinetic Parameters ◽  
Exchange Reaction ◽  
Proton Exchange

scholarly journals Convenient method for estimation of kinetic parameters from time course analysis of enzyme reactions with a microcomputer.

1984 ◽  
Vol 48 (2) ◽  
pp. 533-536 ◽  
Author(s):  
Tadashi YOSHIMOTO ◽  
Futoshi MATSUBARA ◽  
Daisuke TSURU ◽  
Juichiro SHIBASAKI
Keyword(s):  
Kinetic Parameters ◽  
Convenient Method ◽  
Time Course ◽  
Enzyme Reactions
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