ChemInform Abstract: Inhibitory Effects of Polyphenols Toward HCV from the Mangrove Plant Excoecaria agallocha L.

ChemInform ◽  
2012 ◽  
Vol 43 (41) ◽  
pp. no-no ◽  
Author(s):  
Yongxin Li ◽  
Shanjiang Yu ◽  
Dong Liu ◽  
Peter Proksch ◽  
Wenhan Lin
2012 ◽  
Vol 22 (2) ◽  
pp. 1099-1102 ◽  
Author(s):  
Yongxin Li ◽  
Shanjiang Yu ◽  
Dong Liu ◽  
Peter Proksch ◽  
Wenhan Lin

2019 ◽  
Vol 14 (1) ◽  
pp. 29
Author(s):  
Asep Awaludin Prihanto ◽  
Randy Fahrudin Ardiansyah ◽  
Ken Audia Pradarameswari

AbstrakL-asparaginase (EC 3.5.1.1) adalah enzim yang menghidrolisis asam amino L-asparagin menjadi amonia dan asam aspartat. Enzim ini mempunyai manfaat utama dalam bidang farmasi dan industri pangan. Enzim L-asparaginase tersebar secara luas pada mikroorganisme. Mikroorganisme yang mempunyai potensi menghasilkan enzim ini adalah mikroorganisme endofit dari tumbuhan mangrove. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi bakteri endofit penghasil L-asparaginase dari tumbuhan mangrove Buta-buta (E. agallocha). Skrining dilakukan dengan menggunakan medium selektif untuk mendapatkan bakteri penghasil enzim L-asparaginase. Identifikasi molekuler dilakukan dengan menggunakan analisis filogenetik berdasarkan data sekuen 16S rDNA. Dari hasil penelitian ini didapatkan lima isolat bakteri endofit penghasil enzim L-asparaginase, di mana isolat penghasil L-asparaginase tertinggi diidentifikasi secara molekuler. Hasil identifikasi filogenetik molekuler menunjukkan bahwa isolat kode D.104 teridentifikasi sebagai Enterobacter cloacae. Molecular Identification of L-asparaginase-Producing Endophytic Bacteria Isolated from Mangrove Buta-Buta (Excoecaria agallocha)AbstractL-asparaginase (EC 3.5.1.1) is an enzyme which hydrolyze amino acid L-asparagine to aspartate and ammonia. Two main applications of this enzyme are in the pharmaceutical and food industries. The enzyme is widely distributed on microorganism. A potential source of L-asparaginase-producing bacteria is an endophytic bacteria from mangrove plant. This study aimed to isolate and identify L-asparaginase-producing endophytic bacteria from a mangrove plant, E. agallocha (Buta-buta). A screening was carried out using a selective medium to obtain the L-asparaginase enzyme producing bacteria. Molecular identification was carried out using phylogenetic analysis based on 16S rDNA sequence data. In this study, five isolates of the L-asparaginase-producing endophytic bacteria were obtained. The molecular phylogenetic identification showed that the highest L-asparaginase-producing bacterial isolate (code D.104) was identified as Enterobacter cloacae.


Planta Medica ◽  
2012 ◽  
Vol 78 (17) ◽  
pp. 1861-1866 ◽  
Author(s):  
Junfeng Wang ◽  
Zhenyu Lu ◽  
Peipei Liu ◽  
Yi Wang ◽  
Jing Li ◽  
...  

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Md. Abdur Rahman ◽  
Rinku Rani Paul ◽  
Chaina Biswas ◽  
Hakima Akter ◽  
Razina Rouf ◽  
...  

Plants act as a rich source of novel natural pesticides. In the backdrop of the recent revival of interest in developing plant-based insecticides, this study was carried out to investigate the pesticidal activity of Sundarban mangrove plants. A total of nine different plant parts from five plants, namely, Aegiceras corniculatum, Excoecaria agallocha, Heritiera fomes, Xylocarpus moluccensis, and Xylocarpus granatum, were extracted with methanol and tested for insecticidal activity against two common stored product pests Sitophilus oryzae and Sitophilus zeamais using direct contact feeding deterrent wafer disc method. Three bark extracts from A. corniculatum, E. agallocha, and H. fomes showed potent and statistically significant insecticidal activity against both S. oryzae and S. zeamais pests (80–100% mortality). All the active bark extracts were further fractionated using C-18 solid-phase extraction (SPE) columns and tested for their insecticidal activity against S. oryzae pest to identify the active fraction. Only the SPE4 fraction (100% MeOH) from all the three active plants showed the activity against S. oryzae pest with a lethal concentration 50% (LC50) value of 0.5, 1.0, and 1.5 mg/disc for A. corniculatum, E. agallocha, and H. fomes, respectively. The active fraction of A. corniculatum was further profiled for identification of active compounds using LC-ESI-MS and identified (along with some unknown peaks) two previously reported compounds at m/z 625.17630 (isorhamnetin 3-O-rutinoside) and 422.25346 (paspaline) as major constituents. Insecticidal activities of these plants are reported in this study for the first time and would be useful in promoting research aiming for the development of new biopesticides from mangrove plants.


Author(s):  
Deepa Mundekkad ◽  
Chidambaram Ramalingam

Objective: Ornithine decarboxylase (ODC) is one of the key enzymes involved in polyamine synthesis that has a prominent regulatory role in cell growth in prokaryotes and eukaryotes. Its regulation/inhibition is a potential means of controlling cell growth. The purpose of the study was to check the effect of the methanol extracts of leaves and seeds of the mangrove plant Excoecaria agallocha in controlling/inhibiting ODC enzyme produced by Escherichia coli.Methods: The methanol extracts of leaves and seeds of the mangrove plant E. agallocha were made. The inhibitory effect of the extracts on ODC enzyme produced by E. coli was tested by a modified spectrophotometric assay (measured in terms of µM of putrescine produced), and the antibacterial effect of the extract was determined by well diffusion assay.Results: It was found that both the leaf and seed extracts inhibited ODC activity thereby preventing the conversion of ornithine to putrescine (the prominent by-product of ODC activity) in a concentration-dependent manner. The leaf extract exhibited 50% inhibition of ODC activity at a concentration of 17.5 µg/ml whereas seed extract inhibited 50% of the activity at a concentration of 16.1 µg/ml. In the growth inhibition assay by well diffusion method, the effect of the extracts on the growth of E. coli was studied and a concentration-dependent growth inhibition was found - 100 µl of leaf extract showed a zone of inhibition of 12.31 mm (diameter) and seed extract showed 12.64 mm, confirming the antibacterial activity of the extracts.Conclusion: The ODC inhibition-mediated growth inhibition of E. coli is a novel finding as far as the extracts of E. agallocha are concerned. The extract was potent enough to be suggested as an antibacterial agent.Keywords: Ornithine decarboxylase, Excoecaria agallocha, Escherichia coli, Ornithine decarboxylase inhibition, Putrescine, Ornithine, Antibacterial effect.


2013 ◽  
Vol 8 (1-2) ◽  
pp. 35-47 ◽  
Author(s):  
MR Rahman ◽  
M Asaduzzaman

Sundarban is the largest mangrove wetland in the world. It covers an area of about 1mha, of which 60% is located in Bangladesh and the remaining western portion, comprising 40%, lies in India. Mangrove ecosystems are of great ecological significance in the tropical and sub-tropical coast. They protect our coast from heavy wind, tidal waves, coastal erosion and sea water intrusion, generate substantial quantities of fishery resources and provide many useful forestry products. The Sundarban ecosystem supports rich fisheries diversity. This ecosystem support 27 families and 53 species of pelagic fish, 49 families 124 species of demersal fish, 5 families and 24 species of shrimps, 3 families and 7 species of crabs, 8 species of lobster. A total 334 plants, 165 algal, 13 special orchids, 17 fern, 87 monocotyledon and 230 dicotyledon belonging to 245 genera and 75 families from the sundarbans and adjacent area are found available. The principal tree species is Sundry (Heritiera fomes) which covers about 73% to total landmass and the second species is Gewa (Excoecaria agallocha) which covers about 16% of total forest area. The plant species include 35 legumes, 29 grasses, 19 sedges, and 18 euphorbias. Of the 50 true mangrove plant species recorded throughout the globe, the Sundarbans alone contain 35 species. The magnificent among the animals on land is Royal Bengal Tiger, Spotted deer, barking deer and wild boars are there in plenty. Besides those jungle cats, fishing cat, civet cat, monkey, bengal fox, jackle, water monitor, monitor lizard and snakes are important faunal spp. Moreover, abundant of the Sundarbans are purple heron, pond heron, cattle egret, little egret, open billed stork, smaller adjutant stork, brahmini kite, spotted dove, rose ringed parakeet, crow pheasant, wood pecker, bee eater, drongo, pide myna, jungle myna, bulbul, tailor bird, magpie robin, sparrow etc., Otherwise, recorded that wild Buffalo, 2 species of deer, javan rhinoceros extinct and presently 2 species of amphibians, 14 species of reptiles 25 species of birds and 5 species of mammals are considered as endangered species. This paper is to produce a new assessment of the mangroves ecology of Sundarbans. DOI: http://dx.doi.org/10.3329/jsf.v8i1-2.14618 J. Sci. Foundation, 8(1&2): 35-47, June-December 2010


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