Identification of Group 2 Innate Lymphoid Cells in Mouse Lung, Liver, Small Intestine, Bone Marrow, and Mediastinal and Mesenteric Lymph Nodes

ABSTRACT Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly prescribed for a variety of inflammatory conditions; however, the benefits of this class of drugs are accompanied by deleterious side effects, most commonly gastric irritation and ulceration. NSAID-induced ulceration is thought to be exacerbated by intestinal microbiota, but previous studies have not identified specific microbes that contribute to these adverse effects. In this study, we conducted a culture-independent analysis of ∼1,400 bacterial small-subunit rRNA genes associated with the small intestines and mesenteric lymph nodes of rats treated with the NSAID indomethacin. This is the first molecular analysis of the microbiota of the rat small intestine. A comparison of clone libraries and species-specific quantitative PCR results from rats treated with indomethacin and untreated rats revealed that organisms closely related to Enterococcus faecalis were heavily enriched in the small intestine and mesenteric lymph nodes of the treated rats. These data suggest that treatment of NSAID-induced ulceration may be facilitated by addressing the microbiological imbalances.

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Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.

Journal of Experimental Medicine ◽

10.1084/jem.161.3.475 ◽

1985 ◽

Vol 161 (3) ◽

pp. 475-489 ◽

Cited By ~ 254

Author(s):

S H Lee ◽

P M Starkey ◽

S Gordon

Keyword(s):

Bone Marrow ◽

Small Bowel ◽

Lymph Nodes ◽

Large Bowel ◽

Adult Mouse ◽

Specific Antigen ◽

Lymphoid Tissues ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph ◽

Mouse Tissues

We have estimated the macrophage content of different tissues of the normal adult mouse using F4/80, a highly specific antigen marker for mature mouse macrophages. An absorption indirect binding assay was used to quantitate F4/80 antigen against a calibration standard made from the J774.2 macrophage-like cell line. The richest sources of tissue F4/80 antigen were found to be bone marrow, spleen, cervical and mesenteric lymph nodes, large bowel, liver, kidneys, and small bowel. The organs that have the highest total F4/80 antigen content are the liver, large bowel, small bowel, bone marrow, spleen, cervical and mesenteric lymph nodes, and kidney. We conclude that the mononuclear phagocyte system is mainly distributed in the gastrointestinal tract and liver, followed by hemopoietic and lymphoid tissues.

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Sonographic findings of pinpoint hyperechoic foci in the small intestine, liver, and mesenteric lymph nodes are indicative of canine Heterobilharzia americana infection

Veterinary Radiology & Ultrasound ◽

10.1111/vru.12874 ◽

2020 ◽

Author(s):

Valentina S. Moshnikova ◽

Lindsey J. Gilmour ◽

Audrey K. Cook ◽

Michelle Fabiani

Keyword(s):

Small Intestine ◽

Lymph Nodes ◽

Mesenteric Lymph Nodes ◽

Mesenteric Lymph

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The structure of the thymus and mesenteric lymph nodes of newborn rats as a result of the prenatal influence of ethanol

Pediatrician (St Petersburg) ◽

10.17816/ped6451-55 ◽

2015 ◽

Vol 6 (4) ◽

pp. 51-55 ◽

Cited By ~ 1

Author(s):

Petr Vladimirovich Pugach ◽

Sergey Vladimirovich Kruglov ◽

Natalia Rafailovna Karelina ◽

Dmitriy Vitalievich Breusenko ◽

Stepan Yurevich Bazhin ◽

...

Keyword(s):

Lymph Nodes ◽

Morphological Changes ◽

Alcohol Intoxication ◽

Sole Source ◽

Structural Features ◽

Lymphoid Cells ◽

Newborn Rats ◽

Mesenteric Lymph Nodes ◽

White Rats ◽

Mesenteric Lymph

The present study was undertaken to examine the structural features of the thymus and cranial mesenteric lymph nodes of newborn rats that have occurred as a result of antenatal alcohol intoxication. We used a set of morphometric, anatomical and histological methods. A study of the cranial mesenteric lymph nodes performed on 45 newborn rats born to 18 female mongrel white rats seven months of age. The studies were conducted in compliance with the order of the Ministry of Health of the USSR N 755 from 12.08.1977 and the order of the Ministry of Higher and Secondary Special Education of the USSR from 13.11.1984, "On the rules of work with experimental animals". Females, on which was received investigated offspring exposed to 15 % ethanol as the sole source of fluid for 1 week, one and three months before pregnancy, during pregnancy and after its completion. It is shown that depending on the duration of pregravid exposure to ethanol occur progredient changes in the structure of the thymus and cranial mesenteric lymph nodes. In the thymus, as well as in the lymph nodes, decreases the number of lymphoid cells and stromal elements content increases. In lymph nodes there are significant changes in the structure of the sinus system. The severity of the identified morphological changes due to the duration of the effects of alcohol on the system "mother-placenta-fetus”.

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Fulminant Liver Failure with Necrotizing Foci in the Liver, Spleen and Lymph Nodes in Celiac Disease Due to Malignant Lymphoma

Canadian Journal of Gastroenterology ◽

10.1155/1996/637957 ◽

1996 ◽

Vol 10 (4) ◽

pp. 225-229 ◽

Cited By ~ 10

Author(s):

Hugh James Freeman

Keyword(s):

Celiac Disease ◽

T Cell ◽

Lymph Nodes ◽

Liver Failure ◽

Cell Receptor ◽

Lymphoid Cells ◽

Mesenteric Lymph Nodes ◽

Rare Type ◽

Mesenteric Lymph ◽

Spleen And Lymph Nodes

A 65-year-old female with celiac disease developed cholestatic jaundice and fatal liver failure. Investigations revealed widespread necrotic foci in the liver, spleen and mesenteric lymph nodes, changes reminiscent of the mesenteric lymph node cavitation syndrome, which is known to complicate celiac disease. In addition, malignant lymphoid cells were present infiltrating hepatic sinusoids, lymph nodes and spleen. These features are typical of hepatosplenic lymphoma, a rare type of peripheral T cell lymphoma with T cell receptor rearrangement. Lymphorecticular malignancy complicating celiac disease may present with fulminant liver disease.

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An ST2-dependent role of bone marrow-derived group 2 innate lymphoid cells in pulmonary fibrosis

The Journal of Pathology ◽

10.1002/path.5092 ◽

2018 ◽

Vol 245 (4) ◽

pp. 399-409 ◽

Cited By ~ 10

Author(s):

Yuyue Zhao ◽

Francina Gonzalez De Los Santos ◽

Zhe Wu ◽

Tianju Liu ◽

Sem H Phan

Keyword(s):

Bone Marrow ◽

Pulmonary Fibrosis ◽

Innate Lymphoid Cells ◽

Lymphoid Cells ◽

Group 2

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Rotavirus A associated pathology of intestine and mesenteric lymph nodes and occurrence in bovine calves of Gwalior and Bareilly regions

Indian Journal of Animal Research ◽

10.18805/ijar.b-3411 ◽

2018 ◽

Author(s):

Gayatri Kashyap ◽

R. Singh ◽

Y. S. Malik ◽

R. K. Agrawal ◽

K. P. Singh ◽

...

Keyword(s):

Small Intestine ◽

Lymph Nodes ◽

Mononuclear Cells ◽

Dairy Farm ◽

Rt Pcr ◽

Mesenteric Lymph Nodes ◽

Fecal Samples ◽

Rotavirus A ◽

Mesenteric Lymph ◽

Vp6 Gene

To understand the pathology of natural cases of rotavirus (RVA) in bovine calves, a total of 40 cases below 6 months died due to diarrhoea were studied, out of which 7 cases (17.5%) turned positive for RVA by RT-PCR. Histopathology of small intestine showed loss of villous enterocytes, blunting and fusion of villi, elongation of crypts and mononuclear cells infiltration in the lamina-propria. The mesenteric lymph nodes were severely depleted of lymphocytes. These changes were corroborated with presence of RVA antigen in sections by dFAT and nucleic acid by RT-PCR. The fluorescent signals were more in mesenteric lymph nodes than in intestine. Besides, 115 rectal fecal samples were also collected from calves for RVA detection by RT-PCR using VP6 gene specific sets of primers. Dead carcasses of calves (n= 40) belonged to organized dairy farm of Bareilly, while rectal fecal samples belonged to both organized (n= 38) and unorganized farms (n= 77) of Bareilly and Gwalior. The overall occurrence of RVA was 19.3% (30/155), comprising 5/37 cases (13.5%) from Gwalior (MP) and 25/118 cases (21.1%) from Bareilly (UP). These findings suggest the infection of RVA widely prevalent in calves and have potential to escape from the intestinal site to mesenteric lymph nodes.

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CCR7-dependent trafficking of RORγ+ ILCs creates a unique microenvironment within mucosal draining lymph nodes

Nature Communications ◽

10.1038/ncomms6862 ◽

2015 ◽

Vol 6 (1) ◽

Cited By ~ 115

Author(s):

Emma C. Mackley ◽

Stephanie Houston ◽

Clare L. Marriott ◽

Emily E. Halford ◽

Beth Lucas ◽

...

Keyword(s):

Lymph Nodes ◽

Lymphoid Cells ◽

Intestinal Cells ◽

Lymphoid Tissues ◽

Mesenteric Lymph Nodes ◽

Cell Responses ◽

Mesenteric Lymph ◽

Peripheral Lymph ◽

Group 3 ◽

Draining Lymph Nodes

Abstract Presentation of peptide:MHCII by RORγ-expressing group 3 innate lymphoid cells (ILC3s), which are enriched within gut tissue, is required for control of CD4 T-cell responses to commensal bacteria. It is not known whether ILC populations migrate from their mucosal and peripheral sites to local draining secondary lymphoid tissues. Here we demonstrate that ILC3s reside within the interfollicular areas of mucosal draining lymph nodes, forming a distinct microenvironment not observed in peripheral lymph nodes. By photoconverting intestinal cells in Kaede mice we reveal constitutive trafficking of ILCs from the intestine to the draining mesenteric lymph nodes, which specifically for the LTi-like ILC3s was CCR7-dependent. Thus, ILC populations traffic to draining lymph nodes using different mechanisms.

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