scholarly journals Sustainable flow‐synthesis of (bulky)nucleoside drugs by a novel and highly stable nucleoside phosphorylase immobilized on reusable supports

ChemSusChem ◽  
2021 ◽  
Author(s):  
Ana I. Benítez-Mateos ◽  
Francesca Paradisi
Keyword(s):  
Nucleoside Phosphorylase ◽  
Flow Synthesis ◽  
Nucleoside Drugs

Flow-Synthesis of Nucleosides Catalyzed by an Immobilized Purine Nucleoside Phosphorylase fromAeromonas hydrophila: Integrated Systems of Reaction Control and Product Purification

2015 ◽  
Vol 357 (11) ◽  
pp. 2520-2528 ◽  
Author(s):  
Enrica Calleri ◽  
Giulia Cattaneo ◽  
Marco Rabuffetti ◽  
Immacolata Serra ◽  
Teodora Bavaro ◽  
...  
Keyword(s):  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Integrated Systems ◽  
Nucleoside Phosphorylase ◽  
Flow Synthesis

Integration of flow synthesis hardware with on-line purification

2020 ◽  
Author(s):  
David Stout ◽  
Jeremiah Malerich ◽  
Peter Madrid ◽  
Dominique Tartar
Keyword(s):  
Flow Synthesis ◽  
On Line

Purine Nucleoside Phosphorylase as a Molecular Target to Develop Active Compounds Against Mycobacterium Tuberculosis

2010 ◽  
Vol 1 (1) ◽  
pp. 34
Author(s):  
Rodrigo G. Ducati ◽  
André A. Souto ◽  
Rafael A. Caceres ◽  
Walter F. De Azevedo ◽  
Luiz A. Basso ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Purine Nucleoside Phosphorylase ◽  
Molecular Target ◽  
Purine Nucleoside ◽  
Active Compounds ◽  
Nucleoside Phosphorylase

Substrate Specificity of a Purine Nucleoside Phosphorylase from Aeromonas hydrophila Toward 6-Substituted Purines and its Use as a Biocatalyst in the Synthesis of the Corresponding Ribonucleosides

2015 ◽  
Vol 19 (22) ◽  
pp. 2220-2225 ◽  
Author(s):  
Daniela Ubiali ◽  
Carlo Morelli ◽  
Marco Rabuffetti ◽  
Giulia Cattaneo ◽  
Immacolata Serra ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Aeromonas Hydrophila ◽  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Nucleoside Phosphorylase

Nucleosides and Nucleoside Analogues as Emerging Antiviral Drugs

2020 ◽  
Vol 17 ◽  
Author(s):  
Mohamed Ibrahim Elzagheid
Keyword(s):  
Significant Role ◽  
Mode Of Action ◽  
Crucial Role ◽  
Viral Infections ◽  
Antiviral Drugs ◽  
Review Article ◽  
Nucleoside Analogues ◽  
The Past ◽  
Nucleoside Drugs

: Nucleosides and their analogues have been in use for many years and have become essential for treating patients with viral infections. Many additional nucleoside drugs have been approved over the past decades. This strongly demonstrates how important these compounds are and the crucial role they play. Given that a significant amount of research and literature has been documented regarding nucleoside analogues, this review article mainly focuses the discussion on nucleosides and nucleoside analogous that have proven to play significant role or be emerging in the treatment of known viral infections. This covers the names, structures, applications, toxicity, and mode of action of relevant nucleoside analogues.

Purine nucleoside phosphorylase: Isolation and characterization

1990 ◽  
Vol 55 (12) ◽  
pp. 2987-2999 ◽  
Author(s):  
Katarina Šedivá ◽  
Ivan Votruba ◽  
Antonín Holý ◽  
Ivan Rosenberg
Keyword(s):  
Molecular Weight ◽  
Purine Nucleoside Phosphorylase ◽  
Purine Nucleoside ◽  
Leukemia Cells ◽  
Ph Optimum ◽  
Nucleoside Phosphorylase ◽  
Isolation And Characterization ◽  
Reaction Proceeds ◽  
The Matrix ◽  
Sepharose 4B

Purine nucleoside phosphorylase (PNP) from mouse leukemia cells L1210 was purified to homogeneity by a combination of ion exchange and affinity chromatography using AE-Sepharose 4B and 9-(p-succinylaminobenzyl)hypoxanthine as the matrix and the ligand, respectively. The native enzyme has a molecular weight of 104 000 and consists of three subunits of equal molecular weight of 34 000. The results of isoelectric focusing showed that the enzyme is considerably microheterogeneous over the pI-range 4.0-5.8 and most likely consists of eight isozymes. The temperature and pH-optimum of phosphorolysis, purine nucleoside synthesis and also of transribosylation is identical, namely 55 °C and pH 7.4. The transribosylation reaction proceeds in the presence of phosphate only. The following Km-values (μmol l-1) were determined for phosphorolysis: inosine 40, 2'-deoxyinosine 47, guanosine 27, 2'-deoxyguanosine 32. The Km-values (μmol l-1) of purine riboside and deoxyriboside synthesis are lower than the values for phosphorolysis (hypoxanthine 18 and 34, resp., guanine 8 and 11, resp.). An affinity lower by one order shows PNP for (-D-ribose-1-phosphate, (-D-2-deoxyribose-1-phosphate (Km = 200 μmol l-1 in both cases) and phosphate (Km = 805 μmol l-1). The substrate specificity of the enzyme was also studied: positions N(1), C(2) and C(8) are decisive for the binding of the substrate (purine nucleoside).

Direct manipulation of particle size and morphology of ordered mesoporous silica by flow synthesis

RSC Advances ◽  
2015 ◽  
Vol 5 (18) ◽  
pp. 13331-13340 ◽  
Author(s):  
T. N. Ng ◽  
X. Q. Chen ◽  
K. L. Yeung
Keyword(s):  
Particle Size ◽  
Mesoporous Silica ◽  
Hollow Spheres ◽  
Ordered Mesoporous Silica ◽  
Direct Manipulation ◽  
Flow Synthesis ◽  
Precise Control ◽  
Ordered Mesoporous ◽  
Particle Size And Morphology ◽  
Size And Morphology

Flow-synthesis of mesoporous silica allows deliberate and precise control over the size and shapes and enables the preparation of complex microstructures (i.e., hollow spheres).

Continuous flow synthesis of the lamivudine precursor L-Menthyl Glyoxylate

2021 ◽  
Author(s):  
Lais S. D. Azevedo ◽  
Anderson R. Aguillon ◽  
Marcelo T. Lima ◽  
Raquel A. C. Leão ◽  
Rodrigo O. M. A. de Souza
Keyword(s):  
Continuous Flow ◽  
Flow Synthesis
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