scholarly journals Individual behavioral variation reflects personality divergence in the upcoming model organism Nothobranchius furzeri

2018 ◽  
Vol 8 (16) ◽  
pp. 8448-8457 ◽  
Author(s):  
Eli S. J. Thoré ◽  
Laure Steenaerts ◽  
Charlotte Philippe ◽  
Arnout Grégoir ◽  
Luc Brendonck ◽  
...  
Keyword(s):  
Model Organism ◽  
Behavioral Variation ◽  
Nothobranchius Furzeri

scholarly journals Age-Related Alterations in the Behavior and Serotonin-Related Gene mRNA Levels in the Brain of Males and Females of Short-Lived Turquoise Killifish (Nothobranchius furzeri)

Biomolecules ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1421
Author(s):  
Valentina S. Evsiukova ◽  
Elizabeth A. Kulikova ◽  
Alexander V. Kulikov
Keyword(s):  
Locomotor Activity ◽  
Body Mass ◽  
Mrna Level ◽  
Model Organism ◽  
The Body ◽  
Suitable Model ◽  
Mrna Levels ◽  
Age Related ◽  
Nothobranchius Furzeri ◽  
Males And Females

Short-lived turquoise killifish (Nothobranchius furzeri) have become a popular model organism for neuroscience. In the present paper we study for the first time their behavior in the novel tank diving test and the levels of mRNA of various 5-HT-related genes in brains of 2-, 4- and 6-month-old males and females of N. furzeri. The marked effect of age on body mass, locomotor activity and the mRNA level of Tph1b, Tph2, Slc6a4b, Mao, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes in the brains of N. furzeri males was shown. Locomotor activity and expression of the Mao gene increased, while expression of Tph1b, Tph2, Slc6a4b, Htr1aa, Htr2a, Htr3a, Htr3b, Htr4, Htr6 genes decreased in 6-month-old killifish. Significant effects of sex on body mass as well as on mRNA level of Tph1a, Tph1b, Tph2, Slc6a4b, Htr1aa, 5-HT2a, Htr3a, Htr3b, Htr4, and Htr6 genes were revealed: in general both the body mass and the expression of these genes were higher in males. N. furzeri is a suitable model with which to study the fundamental problems of age-related alterations in various mRNA levels related with the brains 5-HT system.

scholarly journals A miRNA catalogue and ncRNA annotation of the short-living fish Nothobranchius furzeri

2017 ◽  
Author(s):  
Mario Baumgart ◽  
Emanuel Barth ◽  
Aurora Savino ◽  
Marco Groth ◽  
Philipp Koch ◽  
...  
Keyword(s):  
Gasterosteus Aculeatus ◽  
Model Organism ◽  
Takifugu Rubripes ◽  
Aging Research ◽  
Control Of Gene Expression ◽  
Non Coding Rna ◽  
Nothobranchius Furzeri ◽  
In Captivity ◽  
Supplementary Material

ABSTRACTBackground: The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age-dependent control of gene expression.Results: Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) for Nothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of seven Nothobranchius species. Additionally, duplication and clustering of N. furzeri miRNAs was analyzed and compared to the four fish species Danio rerio, Oryzias latipes, Gasterosteus aculeatus and Takifugu rubripes. A peculiar characteristic of N. furzeri as compared to other teleosts was a duplication of the miR-29 cluster.Conclusion: The completeness of the catalogue we provide is comparable to that of zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species.Availability: All supplementary material can be found online at http://www.rna.uni-jena.de/en/supplements/nothobranchius-furzeri-mirnome/.

Immunolocalization of S100-like protein in the brain of an emerging model organism: Nothobranchius furzeri

2011 ◽  
Vol 75 (4) ◽  
pp. 441-447 ◽  
Author(s):  
Livia D'Angelo ◽  
Paolo De Girolamo ◽  
Alessandro Cellerino ◽  
Eva Terzibasi Tozzini ◽  
Ettore Varricchio ◽  
...  
Keyword(s):  
Model Organism ◽  
Nothobranchius Furzeri ◽  
The Brain

scholarly journals Ontogenetic Pattern Changes of Nucleobindin-2/Nesfatin-1 in the Brain and Intestinal Bulb of the Short Lived African Turquoise Killifish

2019 ◽  
Vol 9 (1) ◽  
pp. 103 ◽  
Author(s):  
Alessia Montesano ◽  
Elena De Felice ◽  
Adele Leggieri ◽  
Antonio Palladino ◽  
Carla Lucini ◽  
...  
Keyword(s):  
Food Intake ◽  
Model Organism ◽  
Aging Research ◽  
Age Related ◽  
Nothobranchius Furzeri ◽  
Food Intake Regulation ◽  
Mammalian Stomach ◽  
Regulate Food Intake ◽  
Intake Regulation ◽  
The Brain

Nesfatin-1 (Nesf-1) was identified as an anorexigenic and well conserved molecule in rodents and fish. While tissue distribution of NUCB2 (Nucleobindin 2)/Nesf-1 is discretely known in vertebrates, reports on ontogenetic expression are scarce. Here, we examine the age-related central and peripheral expression of NUCB2/Nesf-1 in the teleost African turquoise killifish Nothobranchius furzeri, a consolidated model organism for aging research. We focused our analysis on brain areas responsible for the regulation of food intake and the rostral intestinal bulb, which is analogous of the mammalian stomach. We hypothesize that in our model, the stomach equivalent structure is the main source of NUCB2 mRNA, displaying higher expression levels than those observed in the brain, mainly during aging. Remarkably, its expression significantly increased in the rostral intestinal bulb compared to the brain, which is likely due to the typical anorexia of aging. When analyzing the pattern of expression, we confirmed the distribution in diencephalic areas involved in food intake regulation at all age stages. Interestingly, in the rostral bulb, NUCB2 mRNA was localized in the lining epithelium of young and old animals, while Nesf-1 immunoreactive cells were distributed in the submucosae. Taken together, our results represent a useful basis for gaining deeper knowledge regarding the mechanisms that regulate food intake during vertebrate aging.

scholarly journals Performance of Turquoise killifish, model organism in aging, on commercial pelleted diet: a step towards husbandry standardization

2019 ◽  
Author(s):  
Jakub Žák ◽  
Iva Dyková ◽  
Marin Reichard
Keyword(s):  
Specific Growth ◽  
Model Organism ◽  
Model Organisms ◽  
Reproductive Parameters ◽  
Liver Mass ◽  
Rate Condition ◽  
Chironomid Larvae ◽  
Starting Point ◽  
Nothobranchius Furzeri ◽  
Pelleted Diet

SUMMARYDietary alteration is one of the most universally effective aging interventions, making its standardization a fundamental need for model organisms in aging. Here we address the current lack of standardized formulated diet for Turquoise Killifish Nothobranchius furzeri – a promising model organism. We first demonstrated that N. furzeri can be fully weaned onto a standardized commercially available pelleted diet as the sole nutrition when kept in social tanks. We then compared nine somatic and six reproductive parameters between fish fed a typical laboratory diet - frozen chironomid larvae (bloodworms) and fish fed solely on BioMar pellets. Killifish readily consumed the pellets. Although fish consumed 7.5 times less food mass in the form of pellets than bloodworms, they had comparable somatic and reproductive performance. There was no difference between diet groups in body size, specific growth rate, condition or extent of hepatocellular vacuolation. Fish fed a pelleted diet had higher juvenile body mass and more visceral fat. Pellet-fed males had lower liver mass and possessed a lipid type of hepatocellular vacuolation instead of the prevailing glycogen-like vacuolation in the bloodworm-fed group. No significant effect was found on reproductive parameters. The negligible differences between dietary groups and good acceptance of pellets indicates their suitability as a useful starting point for diet standardization (and potential manipulation) in Nothobranchius furzeri.

scholarly journals Sperm cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Luca Dolfi ◽  
Tsz Kin Suen ◽  
Roberto Ripa ◽  
Adam Antebi
Keyword(s):  
In Vitro Fertilization ◽  
Model Organism ◽  
Current Method ◽  
Inbred Strains ◽  
Vitro Fertilization ◽  
Long Term Storage ◽  
Nothobranchius Furzeri ◽  
Space Requirements

AbstractOver the last decade, the African turquoise killifish, Nothobranchius furzeri, has emerged as an important model system for the study of vertebrate biology and ageing. Propagation of laboratory inbred strains of Nothobranchius furzeri, such as GRZ, however, can pose challenges due to the short window of fertility, the efforts and space requirements involved in continuous strain maintenance, and the risks of further inbreeding. The current method for long term strain preservation relies on arrest of embryos in diapause. To create an alternative for long term maintenance, we developed a robust protocol to cryopreserve and revive sperm for in vitro fertilization (IVF). We tested a variety of extender and activator buffers for sperm IVF, as well as cryoprotectants to achieve practical long-term storage and fertilization conditions tailored to this species. Our protocol enabled sperm to be preserved in a cryogenic condition for months and to be revived with an average of 40% viability upon thawing. Thawed sperm were able to fertilize nearly the same number of eggs as natural fertilization, with an average of ~ 25% and peaks of ~ 55% fertilization. This technical advance will greatly facilitate the use of N. furzeri as a model organism.

scholarly journals Age-Dependent Regulation of Notch Family Members in the Neuronal Stem Cell Niches of the Short-Lived Killifish Nothobranchius furzeri

2021 ◽  
Vol 9 ◽  
Author(s):  
Sara Bagnoli ◽  
Eva Terzibasi Tozzini
Keyword(s):  
Notch Pathway ◽  
Model Organism ◽  
Active Regions ◽  
Proliferative Potential ◽  
Neuronal Stem Cell ◽  
Nothobranchius Furzeri ◽  
Age Dependent ◽  
Embryonic Origin ◽  
Stem Cell Niches

Background: The annual killifish Nothobranchius furzeri is a new experimental model organism in biology, since it represents the vertebrate species with the shortest captive life span and also shows the fastest maturation and senescence recorded in the laboratory. Here, we use this model to investigate the age-dependent decay of neurogenesis in the telencephalon (brain region sharing the same embryonic origin with the mammalian adult niches), focusing on the expression of the Notch pathway genes.Results: We observed that the major ligands/receptors of the pathway showed a negative correlation with age, indicating age-dependent downregulation of the Notch pathway. Moreover, expression of notch1a was clearly limited to active neurogenic niches and declined during aging, without changing its regional patterning. Expression of notch3 is not visibly influenced by aging.Conclusion: Both expression pattern and regulation differ between notch1a and notch3, with the former being limited to mitotically active regions and reduced by aging and the latter being present in all cells with a neurogenic potential, regardless of the level of their actual mitotic activity, and so is less influenced by age. This finally suggests a possible differential role of the two receptors in the regulation of the niche proliferative potential throughout the entire fish life.

scholarly journals Optimization of cryopreservation and in vitro fertilization techniques for the African turquoise killifish Nothobranchius furzeri

2020 ◽  
Author(s):  
Luca Dolfi ◽  
Tsz Kin Suen ◽  
Roberto Ripa ◽  
Adam Antebi
Keyword(s):  
Model Organism ◽  
Vitro Fertilization ◽  
Long Term Storage ◽  
Nothobranchius Furzeri ◽  
Transgenic Lines ◽  
Unreliable Method ◽  
Different Strains ◽  
Term Storage

AbstractOver the last decade, the African turquoise killifish, Nothobranchius furzeri, has emerged as an important model system for the study of vertebrate biology and ageing. However, rearing this fish in captivity can pose challenges, due to the short window of fertility, inbreeding problems, and the continuous maintenance of different strains and transgenic lines. To date, the main means of long term strain maintenance is to arrest embryos in diapause, a poorly understood and unreliable method. To solve these problems, we developed a robust protocol to cryopreserve sperm and to revive them for in vitro fertilization (IVF), as a better option for long term storage of N. furzeri lines. We tested a variety of extender and activator buffers for sperm in vitro fertilization, as well as cryoprotectants to achieve maximal long term storage and fertilization conditions tailored to this species. Our optimized protocol was able to preserve sperm in a cryogenic condition for months and to revive an average of 40% upon thawing. Thawed sperm were able to fertilize nearly the same number of eggs as natural fertilization, with an average of ~25% and peaks of ~55% fertilization. This technical advance will greatly facilitate the use of N. furzeri as a model organism.

scholarly journals Nothobranchius furzeri, an 'instant' fish from an ephemeral habitat

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Martin Reichard ◽  
Matej Polačik
Keyword(s):  
Laboratory Animal ◽  
Model Organism ◽  
Active Phase ◽  
Harsh Environment ◽  
Developmental Arrest ◽  
Active Life ◽  
Nothobranchius Furzeri ◽  
Ephemeral Habitat ◽  
Vertebrate Model ◽  
The Ideal

The turquoise killifish, Nothobranchius furzeri, is a promising vertebrate model in ageing research and an emerging model organism in genomics, regenerative medicine, developmental biology and ecotoxicology. Its lifestyle is adapted to the ephemeral nature of shallow pools on the African savannah. Its rapid and short active life commences when rains fill the pool: fish hatch, grow rapidly and mature in as few as two weeks, and then reproduce daily until the pool dries out. Its embryos then become inactive, encased in the dry sediment and protected from the harsh environment until the rains return. This invertebrate-like life cycle (short active phase and long developmental arrest) combined with a vertebrate body plan provide the ideal attributes for a laboratory animal.

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