Simultaneous electrokinetic and hydrodynamic injection and sequential stacking featuring sweeping for signal amplification following MEKC during the analysis of rapamycin (sirolimus) in serum samples

2018 ◽  
Vol 39 (20) ◽  
pp. 2590-2597 ◽  
Author(s):  
Ilona Olędzka ◽  
Piotr Kowalski ◽  
Alina Plenis ◽  
Natalia Miękus ◽  
Niels Grabow ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Serum Samples ◽  
Hydrodynamic Injection

Tyramide signal amplification of biotinylated probe in dot-blot hybridization assay for the detection of parvovirus B19 DNA in serum samples

2000 ◽  
Vol 302 (1-2) ◽  
pp. 79-87 ◽  
Author(s):  
Marialuisa Zerbini ◽  
Monica Cricca ◽  
Giovanna Gentilomi ◽  
Simona Venturoli ◽  
Giorgio Gallinella ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Parvovirus B19 ◽  
Blot Hybridization ◽  
Hybridization Assay ◽  
Tyramide Signal Amplification ◽  
Dot Blot ◽  
Serum Samples ◽  
Dot Blot Hybridization

scholarly journals Detection of Q Fever Specific Antibodies Using Recombinant Antigen in ELISA with Peroxidase Based Signal Amplification

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hua-Wei Chen ◽  
Zhiwen Zhang ◽  
Erin Glennon ◽  
Wei-Mei Ching
Keyword(s):  
Membrane Protein ◽  
Outer Membrane Protein ◽  
Signal Amplification ◽  
Q Fever ◽  
Recombinant Antigen ◽  
Antibody Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Fever Patient ◽  
Indirect Immunofluorescent

Currently, the accepted method for Q fever serodiagnosis is indirect immunofluorescent antibody assay (IFA) using the whole cell antigen. In this study, we prepared the recombinant antigen of the 27-kDa outer membrane protein (Com1) which has been shown to be recognized by Q fever patient sera. The performance of recombinant Com1 was evaluated in ELISA by IFA confirmed serum samples. Due to the low titers of IgG and IgM in Q fever patients, the standard ELISA signals were further amplified by using biotinylated anti-human IgG or IgM plus streptavidin-HRP polymer. The modified ELISA can detect 88% (29 out of 33) of Q fever patient sera collected from Marines deployed to Iraq. Less than 5% (5 out of 156) of the sera from patients with other febrile diseases reacted with the Com1. These results suggest that the modified ELISA using Com1 may have the potential to improve the detection of Q fever specific antibodies.

A photoluminescent biosensor based on long-range self-assembled DNA cascades and upconversion nanoparticles for the detection of breast cancer-associated circulating microRNA in serum samples

RSC Advances ◽  
2015 ◽  
Vol 5 (23) ◽  
pp. 18008-18012 ◽  
Author(s):  
Jianming Lan ◽  
Fadi Wen ◽  
Fangmeng Fu ◽  
Xi Zhang ◽  
Shuxian Cai ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Long Range ◽  
Signal Amplification ◽  
Upconversion Nanoparticles ◽  
Circulating Microrna ◽  
Serum Samples ◽  
Self Assembled

A photoluminescent biosensor was developed for the detection of microRNA (miRNA) combined signal amplification of long-range self-assembled DNA cascades with upconversion nanoparticles.

scholarly journals Chemiluminescent Optical Fiber Immunosensor Combining Surface Modification and Signal Amplification for Ultrasensitive Determination of Hepatitis B Antigen

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4912
Author(s):  
Xuexue Xu ◽  
Rongbin Nie ◽  
Jingwen Huang ◽  
Li Yang
Keyword(s):  
Hepatitis B ◽  
Optical Fiber ◽  
Signal Amplification ◽  
Hepatitis B Surface Antigen ◽  
Fiber Surface ◽  
Complex Signal ◽  
Biomarker Detection ◽  
Serum Samples ◽  
Limit Of Quantitation

Optical fiber based immunosensors are very attractive for biomarker detection. In order to improve the sensor response, we propose a promising strategy which combines porous-layer modification of the fiber surface and streptavidin-biotin-peroxidase nano-complex signal amplification in chemiluminescent detection. Two hepatitis B antigens, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg), are used as the targets for analysis using the proposed sensor. Comparing to immunoassays using normal optical fiber sensors, the response of the present sensor is enhanced by a factor of 4.8 and 6.7 for detection of HBsAg and HBeAg, respectively. The limit-of-quantitation of the proposed method is as low as 0.3 fg/mL (0.01 fg/mL) with a wide linear response range of 3 fg/mL–150 ng/mL (0.1 fg/mL–160 ng/mL) for sensing HBsAg (HBeAg). Quantitative determination of HBsAg and HBeAg in human serum samples is performed, showing the applicability of the proposed method for biomarker detection.

scholarly journals An isothermal, non-enzymatic, and dual-amplified fluorescent sensor for highly sensitive DNA detection

2021 ◽  
Vol 40 (1) ◽  
pp. 312-322
Author(s):  
Idorenyin Iwe ◽  
Zhigang Li
Keyword(s):  
Thermal Cycling ◽  
Signal Amplification ◽  
Fluorescent Sensor ◽  
Serum Samples ◽  
Practical Applications ◽  
Highly Sensitive ◽  
Dual Stage ◽  
Catalytic Hairpin Assembly ◽  
Nucleic Acid Assay ◽  
Fetal Bovine

Abstract Sensitive DNA assays are of importance in life science and biomedical engineering, but they are heavily dependent on thermal cycling programs or enzyme-assisted schemes, which require the utilization of bulky devices and costly reagents. To circumvent such requirements, we developed an isothermal enzyme-free DNA sensing method with dual-stage signal amplification ability based on the coupling use of catalytic hairpin assembly (CHA) and Mg2+-dependent deoxyribozyme (DNAzyme). In this study, the sensing system involves a set of hairpin DNA probes for CHA (ensuring the first stage of signal amplification) as well as ribonucleobase-modified molecular beacons that serve as activatable substrates for DNAzymes (warranting the second stage of signal amplification). An experimentally determined detection limit of about 0.5 pM is achieved with a good linear range from 0.5 to 10 pM. The results from spiked fetal bovine serum samples further confirm the reliability for practical applications. The non-thermal cycling, enzyme-free, and dual-amplified features make it a powerful sensing tool for effective nucleic acid assay in a variety of biomedical applications.

Enzyme-free quantification of exosomal microRNA by the target-triggered assembly of the polymer DNAzyme nanostructure

The Analyst ◽  
2018 ◽  
Vol 143 (4) ◽  
pp. 813-816 ◽  
Author(s):  
Dinggeng He ◽  
Luo Hai ◽  
Huizhen Wang ◽  
Ri Wu ◽  
Hung-Wing Li
Keyword(s):  
Cancer Cells ◽  
Cancer Patients ◽  
Signal Amplification ◽  
Culture Medium ◽  
Serum Samples ◽  
Amplification Method ◽  
Exosomal Mirnas ◽  
Free Quantification ◽  
Exosomal Microrna

We herein report an enzyme-free signal amplification method for the detection of exosomal miRNAs in culture medium of cancer cells and serum samples from cancer patients via the target-triggered assembly of polymer DNAzyme.

scholarly journals A Universal Proximity CRISPR Cas12a Assay for Ultrasensitive Detection of Nucleic Acids and Proteins

2019 ◽  
Author(s):  
Yongya Li ◽  
Hayam Mansour ◽  
Yanan Tang ◽  
Feng Li
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Signal Amplification ◽  
Target Recognition ◽  
Homogeneous Solution ◽  
Ultrasensitive Detection ◽  
Primer Extension Reaction ◽  
Serum Samples ◽  
Highly Sensitive ◽  
Assay Design

AbstractHerein, we describe a proximity CRISPR Cas12a assay that harnesses “collateral” single-stranded DNase activity of Cas12a as a universal amplifier for the ultrasensitive detection of nucleic acids and proteins. The target recognition is achieved through proximity binding rather than recognition by CRISPR RNA (crRNA), which allows the flexible assay design and expansion to proteins. A binding-induced primer extension reaction is then used to generate a predesigned CRISPR-targetable sequence as a barcode for signal amplification. We demonstrate that our assay is highly sensitive and universal. As low as 1 fM nucleic acid target could be detected isothermally in a homogeneous solution via the integration with nicking cleavage. We’ve also successfully adapted the assay for the sensitive and wash-free detection of antibodies in both buffer and diluted human serum samples.

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