Automatic detecting and counting magnetic beads-labeled target cells from a suspension in a microfluidic chip

2018 ◽  
Vol 40 (6) ◽  
pp. 897-905 ◽  
Author(s):  
Zhenyu Song ◽  
Mengqi Li ◽  
Bao Li ◽  
Yimo Yan ◽  
Yongxin Song
Keyword(s):  
Microfluidic Chip ◽  
Magnetic Beads ◽  
Target Cells

scholarly journals Magnetic Beads Actuating and Sensing by Light Addressability

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 1019
Author(s):  
Yu-Ping Chen ◽  
Hsin-Yin Peng ◽  
Jia-Long Hong ◽  
Min-Hsien Wu ◽  
Chia-Ming Yang
Keyword(s):  
Surface Modification ◽  
Microfluidic Chip ◽  
Sensor Array ◽  
Magnetic Beads ◽  
High Potential ◽  
Micro Particles ◽  
Ito Glass ◽  
Proper Operation ◽  
2D Images ◽  
Sensing Performance

Micro particles and nanoparticles applied in microfluidics became a popular direction to improve biochip performance. Magnetic beads are the well-studied carriers including manipulation and surface modification, which could be easily to apply in biosensor development. A new methodology combined the actuators and sensor in the same chip all trigged by illumination is proposed in this study. A microfluidic chip with a-Si:H layer and ITO/glass with proper operation could be used to collect magnetic beads firstly and then following the optoelectrical measurement. In the meantime, 2D images for clusters of magnetic beads can be easily achieved with the addressable illumination. This proposed platform could own the high potential for a sensor array with better sensing performance.

scholarly journals In vitro expansion of CD3/TCR- human thymocyte populations that selectively lack CD3 delta gene expression: a phenotypic and functional analysis.

1990 ◽  
Vol 172 (5) ◽  
pp. 1409-1418 ◽  
Author(s):  
A Poggi ◽  
R Biassoni ◽  
N Pella ◽  
F Paolieri ◽  
R Bellomo ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Magnetic Beads ◽  
Nk Cell ◽  
Interleukin 2 ◽  
Cytolytic Activity ◽  
Target Cells ◽  
Gamma Delta ◽  
Proliferating Cells ◽  
Experimental Conditions ◽  
Necrosis Factor Alpha

Highly purified CD1-3-4-8- human thymocytes were obtained by panning techniques combined with cell depletion with antibody-coated magnetic beads. Most of these cells expressed cytoplasmic CD3 antigen, as assessed by mAbs known to react with the CD3 epsilon chain. After culture with low doses of PMA (0.5 ng/ml) and subsequent addition (at 24 h) of recombinant interleukin 2 (rIL-2; 100 U/ml) cells underwent extensive proliferation (40-60-fold of the initial cell input after 2 wk). The majority of the proliferating cells were CD3-TCR-. The remaining cells (5-40%) were represented by CD3+ TCR gamma/delta+ (BB3- A13+) cells. Further removal of CD3+ TCR-gamma/delta+ cells resulted in highly purified CD3- populations that further proliferated in culture with no substantial phenotypic changes. When CD3+ thymocytes were cultured under the same experimental conditions, only CD3+ TCR-alpha/beta+ cells could be detected, thus indicating that PMA did not affect the surface expression of the CD3/TCR complex, but rather induced preferential growth of CD3- thymocytes. Surface marker analysis of cultured CD3- thymocytes showed that they were homogeneously CD7+, whereas low proportions of cells expressed CD2 and CD8 antigens. Among the natural killer (NK) cell markers, CD56 was highly expressed by all cells, whereas CD16, CD57, CD11b, NKH2, and GL183 were absent. Importantly, these cells were different from peripheral NK cells, as 80-95% of them expressed cytoplasmic CD3 antigen. Functional analysis revealed a strong cytolytic activity against both NK-sensitive (K562) and NK-resistant (M14, Daudi) human target cells. In a redirected killing assay against the Fc gamma R+ P815 cells, mAbs specific for triggering molecules including CD3, CD2, and CD16 failed to augment target cell lysis, while a strong cytolytic effect was induced by PHA. In addition, PHA alone or in combination with PMA induced tumor necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) (but not IL-2) production by CD3- thymocytes. Cloning of fresh CD1-3-4-8-thymocytes in the presence of PMA and rIL-2 resulted in CD3-CD56+ clones that displayed a pattern of cytolytic activity and lymphokine production similar to that of the polyclonal populations. Northern blot analysis of transcripts coding for CD3/TCR molecules revealed the presence of CD3 zeta, epsilon, and gamma transcripts, while CD3 delta was undetectable. Mature transcripts for both gamma and delta TCR chains could be detected, whereas no TCR-alpha mRNA and only a truncated (1.0 kb) form of TCR-beta mRNA were revealed.(ABSTRACT TRUNCATED AT 400 WORDS)

scholarly journals Adsorption and isolation of nucleic acids on cellulose magnetic beads using a three-dimensional printed microfluidic chip

2015 ◽  
Vol 9 (6) ◽  
pp. 064118 ◽  
Author(s):  
Lei Zhang ◽  
Rachel N. Deraney ◽  
Anubhav Tripathi
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Magnetic Beads ◽  
Three Dimensional

Modelling Immunomagnetic Cell Capture in CFD

2008 ◽  
Author(s):  
Tobias Baier ◽  
Swaty Mohanty ◽  
Klaus Stefan Drese ◽  
Federica Rampf ◽  
Jungtae Kim ◽  
...  
Keyword(s):  
Fluid Dynamics ◽  
Magnetic Particles ◽  
Magnetic Beads ◽  
Stokes Equations ◽  
Type Equation ◽  
Standard Technique ◽  
Binding Kinetics ◽  
Navier Stokes ◽  
Target Cells ◽  
Cell Capture

The separation of cells from a complex sample by immunomagnetic capture has become a standard technique in the last decade and has also obtained increased attention for microfluidic applications. We present a model that incorporates binding kinetics for the formation of cell-bead complexes, which can easily be integrated into a computational fluid dynamics (CFD) code. The model relies on the three equation types: Navier-Stokes equations governing the fluid dynamics, convection-diffusion equations for non-magnetic cells and a Nernst-Planck type equation governing the temporal evolution of cell-bead complex concentrations. The latter two equations are augmented by appropriate ‘reaction’ terms governing the binding kinetics which is formulated as a population rate balance between creation and annihilation of cell-bead complexes. First, the simulation results show, that by means of the developed approach appropriate parameter sets can be identified which allow for a continuous separation of tagged cells (cell/bead complexes) from non-magnetic particles such as non-target cells entering with the target cells. Moreover tagged cells can be, to a certain extend, separated from unbound beads. Second, the computed concentrations at the outlet show a drastic drop for higher cell/bead complexes beyond a certain number of beads per cell. We show that a critical number of beads per cells exists where the binding is considerably reduced or the reaction cascade ceases completely. This occurs when cell/bead complex have a similar magnetic mobility as the free magnetic beads. The presented CFD model has been applied to the simulation of a generic continuous cell separation system showing that the method facilitates the design of magnetophoretic systems.

scholarly journals Detection of Circulating Endothelial Cells via a Microfluidic Disk

2011 ◽  
Vol 57 (4) ◽  
pp. 586-592 ◽  
Author(s):  
Ken-Chao Chen ◽  
Tai-Ping Lee ◽  
Yu-Cheng Pan ◽  
Chi-Ling Chiang ◽  
Chen-Lin Chen ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Endothelial Cells ◽  
Lupus Erythematosus ◽  
Magnetic Beads ◽  
Mononuclear Cells ◽  
Umbilical Vein ◽  
Circulating Endothelial Cells ◽  
Target Cells ◽  
Systemic Lupus ◽  
Disk System

BACKGROUND Circulating endothelial cells (CECs) in the blood are rare but have been shown to be associated with various diseases. With the ratio of CECs to peripheral blood mononuclear cells (PBMCs) less than 1 part per thousand, their separation from PBMCs and detection are challenging. We present a means of detecting CECs from PBMCs via an economical microfluidic disk with a model cell system [human umbilical vein endothelial cells (HUVECs) in PBMCs], along with demonstration of its efficacy clinically. METHODS To enrich these rare cells, we used immunomagnetic beads and a tailor-made magnet on the disk. CEC-simulating HUVECs, as target cells, were stained with primary anti–CD146-phycoerythrin antibody and bound with secondary antibody on antiphycoerythrin magnetic beads. PBMCs served as nontarget cells and were labeled with anti–CD45-FITC antibody. RESULTS When hundreds of HUVECs were mixed in 106 PBMCs, 95% of spiked HUVECs were detected. This yield also held for 60 HUVEC in <104 PBMCs. We compared data from flow cytometry with that from the disk: CEC counts in 50 μL blood from patients with systemic lupus erythematosus were 61.1 (21.5), significantly higher (P < 0.01) than those of healthy donors, 31.2 (13.3). CONCLUSIONS The count of CECs is a suitable marker for symptoms of systemic lupus erythematosus. The microfluidic disk system should be a viable platform for detection of CECs.

Target Cell Detection via Microfluidic Magnetic Beads Assay

2016 ◽  
Author(s):  
Fan Liu ◽  
Pawan K. C. ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Magnetic Field ◽  
Transit Time ◽  
Target Cell ◽  
Magnetic Beads ◽  
Limit Of Detection ◽  
Magnetic Bead ◽  
Target Cells ◽  
Cell Detection ◽  
Coulter Counters ◽  
The Magnetic Field

We present a novel cell detection device based on a magnetic bead cell assay and microfluidic Coulter counting technology. The device can detect specific target cells ratios, as well as cells size distribution and concentrations. The device consists of two identical micro Coulter counters separated by a fluid chamber where an external magnetic field is applied. Target cells conjugated with magnetic beads are retarded by the magnetic field; transit time of a target cell passing through the second counter is longer than that through the first counter. In comparison, a non-target cell transit through two counters with nearly the same time. We demonstrated the transit time delay increased approximately linearly with the increasing target cell concentration. The limit of detection (LOD) of the assay was estimated to be 5.6% in terms of target cell ratio.

scholarly journals Optical Manipulation of Single Magnetic Beads in a Microwell Array on a Digital Microfluidic Chip

2016 ◽  
Vol 88 (17) ◽  
pp. 8596-8603 ◽  
Author(s):  
Deborah Decrop ◽  
Toon Brans ◽  
Pieter Gijsenbergh ◽  
Jiadi Lu ◽  
Dragana Spasic ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Magnetic Beads ◽  
Optical Manipulation ◽  
Microwell Array ◽  
Digital Microfluidic

An integrated microfluidic system for rapid detection and multiple subtyping of influenza A viruses by using glycan-coated magnetic beads and RT-PCR

Lab on a Chip ◽  
2019 ◽  
Vol 19 (7) ◽  
pp. 1277-1286 ◽  
Author(s):  
Kao-Mai Shen ◽  
Narayana Murthy Sabbavarapu ◽  
Chien-Yu Fu ◽  
Jia-Tsrong Jan ◽  
Jen-Ren Wang ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Rapid Detection ◽  
Influenza A ◽  
Magnetic Beads ◽  
Microfluidic System ◽  
Rt Pcr ◽  
Influenza A Viruses ◽  
Reaction Chambers

A microfluidic chip featuring HA × NA arrayed reaction chambers for RT-PCR was developed for diagnosis and subtyping of influenza A viruses.

Sign in / Sign up

Export Citation Format

Share Document