A process-conditioned and spatially consistent method for reducing systematic biases in modeled streamflow

Reference method for simultaneous calibration of the three and more measurement standards for vertical angle measurement is developed. This method can to use for obtaining the systematic biases of the vertical angles measurements for each of the measuring standards relative of the horizontal plain was averaged from measurement results in time their calibration or comparison. For realization of the reference method was developed the autocollimationel electronic measurement standard for the automatization measurement of the vertical angles SeaLineZero_Standard™ (SLZ_S™). Summary standard deviation (k=1) of the vertical angle measurement relative the horizontal plane, from the results of their calibration by reference method, is not more 0,07ʺ…0,15ʺ. This result was obtained without the use the systematic biases, for each measurement standards, as measurements corrections (with opposite sign). The measuring standards, that were developed and researched, are necessary for obtaining the systematic biases of the vertical angle measurement for total stations and theodolites, that have the normed standard error 0,5ʺ and 1ʺ, when these instruments are calibrating.

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Bioseparations Science and Engineering

10.1093/oso/9780195391817.001.0001 ◽

2015 ◽

Cited By ~ 4

Author(s):

Roger G. Harrison ◽

Paul W. Todd ◽

Scott R. Rudge ◽

Demetri P. Petrides

Keyword(s):

Process Design ◽

Qualitative Description ◽

Engineering Practice ◽

Unit Operation ◽

Science And Engineering ◽

Scientific Application ◽

General Application ◽

Unit Operations ◽

Analysis Computer ◽

Consistent Method

Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field of bioseparations. Current, comprehensive, and concise, it covers bioseparations unit operations in unprecedented depth. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro Designer® is used to analyze and evaluate the production of three important biological products. New to this second edition are updated discussions of moment analysis, computer simulation, membrane chromatography, and evaporation, among others, as well as revised problem sets. Unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals working in or studying bioseparations, and is the premier text in the field.

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Gas-Liquid Chromatographic Determination of Technical Chlordane Residues in Food Crops: Interpretation of Analytical Data

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/58.5.1051 ◽

1975 ◽

Vol 58 (5) ◽

pp. 1051-1061

Author(s):

William P Cochrane ◽

James F Lawrence ◽

Young W Lee ◽

Ronald B Maybury ◽

Brian P Wilson

Keyword(s):

Field Experiments ◽

Stationary Phases ◽

Analytical Data ◽

Chromatographic Determination ◽

Electron Capture Detection ◽

Food Crops ◽

Liquid Chromatographic Determination ◽

Consistent Method ◽

Liquid Chromatographic

Abstract An interlaboratory investigation of technical chlordane residues in food crops was carried out to determine the most practical and consistent method of reporting results. Using a technical chlordane reference standard, 8 gas chromatographic stationary phases were studied for their resolution capabilities. The best separations were obtained with SE-30 and its OV-1 equivalent. Using these columns and electron capture detection, potatoes and carrots from supervised field experiments were analyzed in duplicate and quantitated by using 4 methods of calculation. The data were statistically treated to determine the precision and bias for each method. Also, 1 sample was analyzed in duplicate on 2 different occasions by 6 laboratories to substantiate the initial conclusions. Based on the criterion of high precision it is suggested that a comparison of total area under the chromatogram of the sample with total area of a standard technical chlordane be the method of quantitation. Only peaks which are common to both standard and sample have any significance in this type of calculation.

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SAUTE: sequence assembly using target enrichment

BMC Bioinformatics ◽

10.1186/s12859-021-04174-9 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Alexandre Souvorov ◽

Richa Agarwala

Keyword(s):

De Bruijn Graph ◽

Challenging Problem ◽

Target Enrichment ◽

Rna Seq ◽

Sequencing Technology ◽

Insert Size ◽

Systematic Biases ◽

Target Sequences ◽

Genomic Regions ◽

Higher Sensitivity

Abstract Background Illumina is the dominant sequencing technology at this time. Short length, short insert size, some systematic biases, and low-level carryover contamination in Illumina reads continue to make assembly of repeated regions a challenging problem. Some applications also require finding multiple well supported variants for assembled regions. Results To facilitate assembly of repeat regions and to report multiple well supported variants when a user can provide target sequences to assist the assembly, we propose SAUTE and SAUTE_PROT assemblers. Both assemblers use de Bruijn graph on reads. Targets can be transcripts or proteins for RNA-seq reads and transcripts, proteins, or genomic regions for genomic reads. Target sequences are nucleotide and protein sequences for SAUTE and SAUTE_PROT, respectively. Conclusions For RNA-seq, comparisons with Trinity, rnaSPAdes, SPAligner, and SPAdes assembly of reads aligned to target proteins by DIAMOND show that SAUTE_PROT finds more coding sequences that translate to benchmark proteins. Using AMRFinderPlus calls, we find SAUTE has higher sensitivity and precision than SPAdes, plasmidSPAdes, SPAligner, and SPAdes assembly of reads aligned to target regions by HISAT2. It also has better sensitivity than SKESA but worse precision.

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The application of a new laminitis scoring method to model the rate and pattern of improvement from equine endocrinopathic laminitis in a clinical setting

BMC Veterinary Research ◽

10.1186/s12917-020-02715-7 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

A. Meier ◽

J. McGree ◽

R. Klee ◽

J. Preuß ◽

D. Reiche ◽

...

Keyword(s):

Best Practice ◽

Clinical Status ◽

Clinical Signs ◽

Scoring Method ◽

Pain Medications ◽

Exponential Decay Model ◽

History Of ◽

Consistent Method ◽

Foot Disease ◽

New Treatments

Abstract Background Endocrinopathic, or hyperinsulinaemia-associated laminitis (HAL) is a common and debilitating equine foot disease, and although no pharmacological treatments are registered, several are under development. To evaluate the effect of such treatments, an accurate and consistent method is needed to track the clinical signs of laminitis over time, and the natural history of the disease, in terms of a ‘normal’ pattern of improvement, needs to be understood. This study examined the improvement pattern in clinical cases of naturally-occurring HAL subjected to a range of best-practice interventions, using two different scoring methods. Eighty horses and ponies with suspected HAL were enrolled in a study conducted at 16 veterinary practices across Germany. The severity of laminitis was assessed by independent veterinarians using both the traditional Obel method and a modified Obel method developed by Meier and colleagues. Assessments were made on the day of diagnosis (d 0), then on days 4, 9, 14, 25 and 42 during the intervention period. Pain medications were withheld for 24 h prior to clinical examination in all cases. Results Time to marked improvement from laminitis varied between individuals, but was difficult to monitor accurately using the Obel method, with the median grade being 2/4 on days 0 and 4, then 0/4 from d 9 onwards. More subtle changes could be identified using the Meier method, however, and the median scores were seen to follow the form of an exponential decay model in most horses, improving from 8/12 on d 0, to 0/12 on d 25. Within this composite scoring method, considerable variation was observed in the rate of improvement of individual clinical signs, with the average time taken for each sign to reach a median score of 0 ranging from 4 days (foot lift and weight shifting) to 25 days (gait when turned in a circle) across all 80 horses. Conclusions The Meier method provides a reliable and consistent method for monitoring the clinical status of horses with HAL, and despite the variability, the pattern of improvement described here should provide a useful benchmark against which individual cases and new treatments can be assessed.

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Evaluation of four different HPLC devices for hemoglobinopathy screening

Turkish Journal of Biochemistry ◽

10.1515/tjb-2019-0484 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Müjgan Ercan Karadağ ◽

Emiş Deniz Akbulut ◽

Esin Avcı ◽

Esra Fırat Oğuz ◽

Saadet Kader ◽

...

Keyword(s):

Gold Standard ◽

High Performance ◽

Public Health Problem ◽

Reference Value ◽

Comparison Study ◽

Gold Standard Method ◽

Blood Samples ◽

Systematic Biases ◽

Hemoglobin Variants ◽

Thalassemia Screening

AbstractObjectiveHemoglobinopathies are a common public health problem in Turkey. In the screening of these disorders in population, cation-exchange high performance liquid chromatography (HPLC) is accepted as the gold standard method. In this study, the aim was to assess four different HPLC devices used in hemoglobinopathy screening.Materials and methodsA total of 58 blood samples were analyzed with four different HPLC methods (Bio-Rad variant II, Agilent 1100, Tosoh G8 and Trinity Ultra2 trademarks).ResultsThe comparison study demonstrated a good correlation between the results of each HPLC analyzer and the reference value obtained by averaging all the HbA2 results belonging to the methods tested in the study [ (Tosoh G8 (r=0.988), Bio-Rad variant II (r=0.993), Agilent 1100 (r=0.98) and Trinity Ultra2 (r=0.992) ]. HbA2 determination in the presence of HbE was interfered in both Bio-Rad variant II and Tosoh G8.ConclusionThe analyzers were found to have compatible HbA2 results but with accompanying different degrees of proportional and systematic biases. HPLC analyzers may be affected by different hemoglobin variants at different HbA2 concentrations, which is an important point to take into consideration during the evaluation of HbA2 results in thalassemia screening.

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Towards B-Spline Atomic Structure Calculations

Atoms ◽

10.3390/atoms9030050 ◽

2021 ◽

Vol 9 (3) ◽

pp. 50

Author(s):

Charlotte Froese Fischer

Keyword(s):

Atomic Structure ◽

Bound States ◽

Virial Theorem ◽

B Spline ◽

B Splines ◽

Self Consistent ◽

History Of ◽

Consistent Method ◽

Atomic Structure Calculations ◽

Structure Calculations

The paper reviews the history of B-spline methods for atomic structure calculations for bound states. It highlights various aspects of the variational method, particularly with regard to the orthogonality requirements, the iterative self-consistent method, the eigenvalue problem, and the related sphf, dbsr-hf, and spmchf programs. B-splines facilitate the mapping of solutions from one grid to another. The following paper describes a two-stage approach where the goal of the first stage is to determine parameters of the problem, such as the range and approximate values of the orbitals, after which the level of accuracy is raised. Once convergence has been achieved the Virial Theorem, which is evaluated as a check for accuracy. For exact solutions, the V/T ratio for a non-relativistic calculation is −2.

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