Comparing the impacts of sediment‐spiked cadmium on Chironomidae larvae in laboratory bioassays and field microcosms and the implications for field validation of site‐specific threshold concentrations

2021 ◽  
Author(s):  
Zhihong Liu ◽  
Chi Zhang ◽  
Zhuohang Xin ◽  
Peidong Tai ◽  
Changchun Song ◽  
...  
Keyword(s):  
Field Validation ◽  
Site Specific ◽  
Chironomidae Larvae ◽  
Laboratory Bioassays

Evaluation of copper toxicity using site specific algae and water chemistry: Field validation of laboratory bioassays

2018 ◽  
Vol 155 ◽  
pp. 59-65 ◽  
Author(s):  
Elyssa G. Fawaz ◽  
Darine A. Salam ◽  
Lina Kamareddine
Keyword(s):  
Water Chemistry ◽  
Copper Toxicity ◽  
Field Validation ◽  
Site Specific ◽  
Laboratory Bioassays

Intergovernmental panel on climate change’s landfill methane protocol: Reviewing 20 years of application

2018 ◽  
Vol 36 (9) ◽  
pp. 827-840 ◽  
Author(s):  
Max J Krause
Keyword(s):  
Climate Change ◽  
Organic Carbon ◽  
Landfill Gas ◽  
Emission Inventories ◽  
Field Validation ◽  
Intergovernmental Panel ◽  
Site Specific ◽  
Methane Generation ◽  
National Estimates ◽  
Practical Tool

The Intergovernmental Panel on Climate Change (IPCC) protocol for predicting national methane emission inventories from landfills was published 22 years ago in the 1996 Revised Guidelines. There currently exists a broad dataset to review landfill parameters and reported values and their appropriateness in use and application in a range of site-specific, regional, and national estimates. Degradable organic carbon (DOC) content was found to range from 0.0105 to 0.65 g C/g waste, with an average of 0.166 g C/g waste. The fraction of DOC that would anaerobically degrade (DOC f) was reported to range from 50–83%, whereas higher and lower values have been experimentally determined for a variety of waste components, such as wood (0–50%) and food waste (50–75%). Where field validation occurred for the methane correction factor, values were substantially lower than defaults. The fraction of methane in anaerobic landfill gas ( F) default of 50% is almost universally applied and is appropriate for cellulosic wastes. The methane generation rate constant ( k) varied widely from 0.01 to 0.51 y−1, representing half-lives from 1 to 69 years. Methane oxidation (OX) default values of 0 and 10% may be valid, but values greater than 30% have been reported for porous covers at managed sites. The IPCC protocol is a practical tool with uncertainties and limitations that must be addressed when used for purposes other than developing inventories.

Large-cluster and combined fluorescent and gold immunoprobes

1996 ◽  
Vol 54 ◽  
pp. 892-893
Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  
Keyword(s):  
Metal Cluster ◽  
Sulfonyl Chloride ◽  
Gel Filtration ◽  
Cross Linking ◽  
Site Specific ◽  
Fab Fragments ◽  
Cluster Label ◽  
Covalently Linked ◽  
Texas Red ◽  
Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

The challenge of detecting modifications on proteins

2020 ◽  
Vol 64 (1) ◽  
pp. 135-153 ◽  
Author(s):  
Lauren Elizabeth Smith ◽  
Adelina Rogowska-Wrzesinska
Keyword(s):  
Mass Spectrometry ◽  
Protein Function ◽  
Chemical Properties ◽  
Lysine Acetylation ◽  
Mass Determination ◽  
Post Translational Modifications ◽  
Site Specific ◽  
The Common

Abstract Post-translational modifications (PTMs) are integral to the regulation of protein function, characterising their role in this process is vital to understanding how cells work in both healthy and diseased states. Mass spectrometry (MS) facilitates the mass determination and sequencing of peptides, and thereby also the detection of site-specific PTMs. However, numerous challenges in this field continue to persist. The diverse chemical properties, low abundance, labile nature and instability of many PTMs, in combination with the more practical issues of compatibility with MS and bioinformatics challenges, contribute to the arduous nature of their analysis. In this review, we present an overview of the established MS-based approaches for analysing PTMs and the common complications associated with their investigation, including examples of specific challenges focusing on phosphorylation, lysine acetylation and redox modifications.

CPT Site-Specific Biopsy Codes: Undercoding Means Underpayment

2006 ◽  
Vol 37 (6) ◽  
pp. 49
Author(s):  
Bruce Jancin
Keyword(s):  
Site Specific

MEMORY EFFECTS IN THE FRAGMENTATION OF MOLECULES FOLLOWING SITE-SPECIFIC CORE EXCITATION

1987 ◽  
Vol 48 (C9) ◽  
pp. C9-741-C9-744 ◽  
Author(s):  
W. HABENICHT ◽  
L. A. CHEWTER ◽  
M. SANDER ◽  
K. MÜLLER-DETHLEFS ◽  
E. W. SCHLAG
Keyword(s):  
Memory Effects ◽  
Core Excitation ◽  
Site Specific

Site Specific Comparision of Secondary Metabolites of Stylissa massa by Hplc Chemical Fingerprinting

Planta Medica ◽  
2008 ◽  
Vol 74 (03) ◽  
Author(s):  
S Ankisetty ◽  
M Slattery
Keyword(s):  
Secondary Metabolites ◽  
Site Specific ◽  
Chemical Fingerprinting
Sign in / Sign up

Export Citation Format

Share Document