Phenolic content and antibacterial activity of olive oil waste waters

The annual olive oil production in Cyprus is in the range of 2700–3100 t y−1, resulting in the generation of significant amount of waste. The cocomposting of the olive oil solid residue (OOSR) and the treated wastewaters (with Fenton) from the olive oil production process with the application of reed beds has been studied as an integrated method for the treatment of wastewater containing high organic and toxic pollutants under warm climate conditions. The experimental results indicated that the olive mill wastewater (OMW) is detoxified at the end of the Fenton process. Specifically, COD is reduced up to 65% (minimum 54.32%) by the application of Fenton and another 10–28% by the application of red beds as a third stage. The final cocomposted material of OOSR with the treated olive mile wastewater (TOMW) presents optimum characteristics and is suitable for agricultural purpose.

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A unique phenolic extraction method from olive oil macerate of Hypericum perforatum using DMSO: Assessment of in vitro anticancer activity, LC-MS/MS profile, total phenolic content and antioxidant capacity

South African Journal of Botany ◽

10.1016/j.sajb.2021.01.015 ◽

2021 ◽

Vol 139 ◽

pp. 6-11

Author(s):

Erdal Eroglu ◽

Seyda Nur Girgin

Keyword(s):

Antioxidant Capacity ◽

Olive Oil ◽

Anticancer Activity ◽

Total Phenolic Content ◽

Extraction Method ◽

Hypericum Perforatum ◽

Phenolic Content ◽

Total Phenolic ◽

Phenolic Extraction

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Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

Journal of Fundamental and Applied Pharmaceutical Science ◽

10.18196/jfaps.v2i1.12406 ◽

2021 ◽

Vol 2 (1) ◽

pp. 23-33

Author(s):

Abdul Aziz ◽

Veggy Nadya Yuliawan ◽

Paula Mariana Kustiawan

Keyword(s):

Antibacterial Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Polar Fraction ◽

Hexane Fraction ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Phytochemical Content ◽

Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2 mm ± 1.5) at 200µg/mL concentration.

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Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽

10.21640/ns.v12i24.2134 ◽

2020 ◽

Vol 12 (24) ◽

Author(s):

Rey David Vargas Sánchez ◽

Evelin Martínez Benavidez ◽

Javier Hernández ◽

Gastón Ramón Torrescano Urrutia ◽

Armida Sánchez Escalante

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Phenolic Compounds ◽

Physicochemical Properties ◽

Phenolic Content ◽

Biological Activities ◽

Sensory Characteristics ◽

Total Phenolic ◽

Gram Positive ◽

Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v77.6747 ◽

2015 ◽

Vol 77 (25) ◽

Cited By ~ 1

Author(s):

Nur Azfa Shuib ◽

Anwar Iqbal ◽

Fatimatul Akmal Sulaiman ◽

Izzatie Razak ◽

Deny Susanti

Keyword(s):

Antibacterial Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Diffusion Method ◽

Total Phenolic ◽

Disc Diffusion ◽

Flavonoid Content ◽

Disc Diffusion Method ◽

Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

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