Studies on qualitative and quantitative detection of trehalose purity by terahertz spectroscopy

A dip-and-read microfluidic paper-based analytical device (µPAD) was developed for the qualitative and quantitative detection of the total hardness of water. To create well-defined hydrophobic barriers on filter paper, a regular office printer and a commercially available permanent marker pen were utilized as a quick and simple technique with easily accessible equipment/materials to fabricate µPAD in new or resource-limited laboratories without sophisticated equipment. After a wettability and barrier efficiency analysis on the permanent marker colors, the blue and green ink markers exhibited favorable hydrophobic properties and were utilized in the fabrication of the developed test devices. The device had five reaction and detection zones modeled after the classification given by the World Health Organization (WHO), so qualitatively it determined whether the water was ‘soft’, ‘moderately hard’, ‘hard’, or ‘very hard’ by changing color from blue to pink in about 3 min. The device was also used to introduce an alternative colorimetric reaction for quantitative analysis of the water hardness without the need for ethylenediaminetetraacetic acid (EDTA) and without compromising the simplicity and low cost of the device. The developed µPAD showed a calculated limit of detection (LOD) of 0.02 mM, which is at least 80% less than those of commercially available test strips and other reported µPADs, and the results of the real-world samples were consistent with those of the standard titration (with EDTA). In addition, the device exhibited stability for 2 months at room and frigid condition (4 °C) and at varying harsh temperatures from 25 to 100 °C. The results demonstrate that the developed paper-based device can be used for rapid, on-site analysis of water with no interferences and no need for a pipette for sample introduction during testing.

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Qualitative and quantitative detection using eDNA technology: A case study of Fenneropenaeus chinensis in the Bohai Sea

Aquaculture and Fisheries ◽

10.1016/j.aaf.2020.03.012 ◽

2020 ◽

Vol 5 (3) ◽

pp. 148-155

Author(s):

Miao Li ◽

Xiujuan Shan ◽

Weiji Wang ◽

Xiaosong Ding ◽

Fangqun Dai ◽

...

Keyword(s):

Bohai Sea ◽

Fenneropenaeus Chinensis ◽

Quantitative Detection ◽

Qualitative And Quantitative ◽

The Bohai Sea

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Qualitative and quantitative determination of potassium aluminum sulfate dodecahydrate in potato starch based on terahertz spectroscopy

Microwave and Optical Technology Letters ◽

10.1002/mop.32061 ◽

2019 ◽

Vol 62 (2) ◽

pp. 525-530

Author(s):

Jianjun Liu ◽

Lanlan Fan

Keyword(s):

Quantitative Determination ◽

Aluminum Sulfate ◽

Terahertz Spectroscopy ◽

Potato Starch ◽

Qualitative And Quantitative ◽

Potassium Aluminum Sulfate

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Novel Reference Gene, High-mobility-group protein I/Y, Used in Qualitative and Real-Time Quantitative Polymerase Chain Reaction Detection of Transgenic Rapeseed Cultivars

Journal of AOAC International ◽

10.1093/jaoac/88.2.577 ◽

2005 ◽

Vol 88 (2) ◽

pp. 577-584 ◽

Cited By ~ 22

Author(s):

Haibo Weng ◽

Litao Yang ◽

Zhili Liu ◽

Jiayu Ding ◽

Aihu Pan ◽

...

Keyword(s):

Reference Gene ◽

High Mobility ◽

Quantitative Detection ◽

High Mobility Group Protein ◽

Qualitative And Quantitative ◽

Qualitative And Quantitative Pcr ◽

Group Protein ◽

Endogenous Reference ◽

Polymerase Chain ◽

Transgenic Rapeseed

Abstract With the development of transgenic crops, regulations to label the genetically modified organisms (GMOs) and their derived products have been issued in many countries. Polymerase chain reaction (PCR) methods are thought to be reliable and useful techniques for qualitative and quantitative detection of GMOs. These methods are generally needed to amplify the transgene and compare the amplified results with that of a corresponding reference gene to get the reliable results. Specific primers were developed for the rapeseed (Brassica napus), High-mobility-group protein I/Y (HMG-I/Y) single-copy gene and PCR cycling conditions suitable for the use of this sequence as an endogenous reference gene in both qualitative and quantitative PCR assays. Both methods were assayed with 15 different rapeseed varieties, and identical amplified products were obtained with all of them. No amplification was observed when templates were the DNA samples from the other species of Brassica genus or other species, such as broccoli, stem mustard, cauliflower, Chinese cabbage, cabbage, sprouts, Arabidopsis thaliana, carrot, tobacco, soybean, mung bean, tomato, pepper, eggplant, plum, wheat, maize, barley, rice, lupine, and sunflower. This system was specific for rapeseed. Limits of detection and quantitation in qualitative and quantitative PCR systems were about 13 pg DNA (about 10 haploid genomes) and about 1.3 pg DNA (about 1 haploid genome), respectively. To further test the feasibility of this HMG-I/Y gene as an endogenous reference gene, samples containing transgenic rapeseed GT73 with the inserted glyphosate oxidoreductase (GOX) gene were quantitated. These demonstrated that the endogenous PCR detection systems were applicable to the qualitative and quantitative detection of transgenic rapeseed.

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Establishment and In-House Validation of Simplex and Duplex PCR Methods for Event-Specific Detection of Maize SYN-E3272-5 Using a New Reference Molecule

Journal of AOAC International ◽

10.1093/jaoac/93.2.663 ◽

2010 ◽

Vol 93 (2) ◽

pp. 663-675

Author(s):

Kailin Shen ◽

Xiang Li ◽

Shu Wang ◽

Yingjie Pan ◽

Zhiyi Shi ◽

...

Keyword(s):

Quantitative Pcr ◽

Genetically Modified Organisms ◽

Insertion Site ◽

Quantitative Detection ◽

Duplex System ◽

Detection Techniques ◽

Qualitative And Quantitative ◽

Reference Molecule ◽

Gm Maize ◽

Pcr Assays

Abstract Despite rapid developments in the detection techniques for genetically modified organisms (GMOs), the event-specific PCR method with high specificity is still the most used technique. In this study, event-specific simplex and duplex qualitative and quantitative detection systems were developed targeting the 3 insertion site of GM maize SYN-E3272-5 (3272) construct. A reference molecule p3272 was constructed to act as positive control and as calibrator for quantitative analysis. The LOD for simplex and duplex qualitative PCR assays was 10 copies of p3272 control DNA. LOD and the LOQ for simplex and duplex quantitative PCR assays were 10 and 25 copies of p3272 DNA, respectively. Furthermore, four practical GM maize samples were quantified using the established simplex and duplex quantitative PCR systems by in-house validation. Results from five operators showed that the bias ranged from 3.44 to 17.24 in the simplex system and from 0.42 to 16.06 in the duplex system, respectively. These results demonstrated that the established event-specific simplex and duplex qualitative and quantitative PCR systems combined with the reference molecule p3272 are suitable for the detection of GM maize 3272 and its derived products.

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