Cell death induced byN-(4-hydroxyphenyl)retinamide in human epidermal keratinocytes is modulated by TGF-β and diminishes during the progression of squamous cell carcinoma

2006 ◽  
Vol 119 (12) ◽  
pp. 2803-2811 ◽  
Author(s):  
Maria Davies ◽  
Ian C. Paterson ◽  
Anu Ganapathy ◽  
Stephen S. Prime
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes

scholarly journals Anticancer Efficacy of Nonthermal Plasma Therapy Combined with PD-L1 Antibody Conjugated Gold Nanoparticles on Oral Squamous Cell Carcinoma

2021 ◽  
Vol 11 (10) ◽  
pp. 4559
Author(s):  
Jinyoung Park ◽  
Yoon-Seo Jang ◽  
Jeong-Hae Choi ◽  
Miheon Ryu ◽  
Gyoo-Cheon Kim ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Nonthermal Plasma ◽  
Therapeutic Effects ◽  
Hacat Cells ◽  
Plasma Therapy

Combination therapies for the treatment of oral squamous cell carcinoma have been studied extensively and represent a synergistic approach with better outcomes than monotherapy. In this study, a novel combination therapy was investigated using gold nanoparticles (GNP) conjugated to programmed cell death protein ligand 1 (PD-L1) antibodies and nonthermal plasma (NTP). The present study describes the effectiveness of NTP using PD-L1 antibody conjugated to GNP in PD-L1 expressing SCC-25 cells, an oral squamous cell carcinoma line. Immunocytochemistry revealed higher levels of PD-L1 expression and an increase in the selective uptake of PD-L1 antibody + GNP on SCC-25 cells compared to HaCaT cells. In addition, cell viability analyses confirmed higher levels of cell death of SCC-25 cells after treatment with PD-L1 antibody, GNP, and NTP compared to HaCaT cells. Among the experimental groups, the highest cell death was observed upon treatment with PD-L1 antibody + GNP + NTP. Following the Western blot analysis and immunofluorescence staining, the expression of apoptosis-related proteins was found to increase after treatment with PD-L1 antibody + GNP + NTP among the other experimental groups. In conclusion, the treatment of SCC-25 cells with PD-L1 antibody + GNP + NTP significantly increased the number of dead cells compared to other experimental groups. The results of this in vitro study confirmed the therapeutic effects of PD-L1 antibody + GNP + NTP treatment on oral squamous cell carcinoma.

Kaempferol Induces Cell Death and Sensitizes Human Head and Neck Squamous Cell Carcinoma Cell Lines to Cisplatin

2020 ◽  
Author(s):  
Mabel Catalán ◽  
Catalina Rodríguez ◽  
Ivonne Olmedo ◽  
Javiera Carrasco-Rojas ◽  
Diego Rojas ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Lines ◽  
Squamous Cell ◽  
Carcinoma Cell ◽  
Human Head ◽  
Neck Squamous Cell Carcinoma ◽  
Carcinoma Cell Lines

scholarly journals SET protein accumulation prevents cell death in head and neck squamous cell carcinoma through regulation of redox state and autophagy

2019 ◽  
Vol 1866 (4) ◽  
pp. 623-637 ◽  
Author(s):  
Amanda Tomie Ouchida ◽  
Valéria Tudella Uyemura ◽  
André Lima Queiroz ◽  
Verônica Soares Brauer ◽  
Marinaldo Pacífico Cavalcanti-Neto ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Redox State ◽  
Neck Squamous Cell Carcinoma ◽  
Protein Accumulation

scholarly journals Metformin increases PDH and suppresses HIF-1α under hypoxic conditions and induces cell death in oral squamous cell carcinoma

Oncotarget ◽  
2016 ◽  
Vol 7 (34) ◽  
pp. 55057-55068 ◽  
Author(s):  
Talita Antunes Guimarães ◽  
Lucyana Conceição Farias ◽  
Eliane Sobrinho Santos ◽  
Carlos Alberto de Carvalho Fraga ◽  
Lissur Azevedo Orsini ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Hypoxic Conditions

scholarly journals The mechanism of de novo expression of programmed cell death-ligand 1 in squamous cell carcinoma of the lung

2017 ◽  
Vol 38 (4) ◽  
pp. 2189-2196 ◽  
Author(s):  
Tomoyuki Igarashi ◽  
Koji Teramoto ◽  
Mitsuaki Ishida ◽  
Jun Hanaoka ◽  
Yataro Daigo
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Programmed Cell Death ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
De Novo

scholarly journals Radiation Sensitization of Basal Cell and Head and Neck Squamous Cell Carcinoma by the Hedgehog Pathway Inhibitor Vismodegib

2018 ◽  
Vol 19 (9) ◽  
pp. 2485 ◽  
Author(s):  
Stephanie Hehlgans ◽  
Patrick Booms ◽  
Ömer Güllülü ◽  
Robert Sader ◽  
Claus Rödel ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Squamous Cell Carcinoma ◽  
Cell Death ◽  
Cell Carcinoma ◽  
Head And Neck ◽  
Cell Line ◽  
Squamous Cell ◽  
Hedgehog Signaling ◽  
Cell Cycle Distribution ◽  
Hedgehog Signaling Pathway

Vismodegib, an inhibitor of the Hedgehog signaling pathway, is an approved drug for monotherapy in locally advanced or metastatic basal cell carcinoma (BCC). Data on combined modality treatment by vismodegib and radiation therapy, however, are rare. In the present study, we examined the radiation sensitizing effects of vismodegib by analyzing viability, cell cycle distribution, cell death, DNA damage repair and clonogenic survival in three-dimensional cultures of a BCC and a head and neck squamous cell carcinoma (HNSCC) cell line. We found that vismodegib decreases expression of the Hedgehog target genes glioma-associated oncogene homologue (GLI1) and the inhibitor of apoptosis protein (IAP) Survivin in a cell line- and irradiation-dependent manner, most pronounced in squamous cell carcinoma (SCC) cells. Furthermore, vismodegib significantly reduced proliferation in both cell lines, while additional irradiation only slightly further impacted on viability. Analyses of cell cycle distribution and cell death induction indicated a G1 arrest in BCC and a G2 arrest in HNSCC cells and an increased fraction of cells in SubG1 phase following combined treatment. Moreover, a significant rise in the number of phosphorylated histone-2AX/p53-binding protein 1 (γH2AX/53BP1) foci in vismodegib- and radiation-treated cells was associated with a significant radiosensitization of both cell lines. In summary, these findings indicate that inhibition of the Hedgehog signaling pathway may increase cellular radiation response in BCC and HNSCC cells.

Sign in / Sign up

Export Citation Format

Share Document