scholarly journals Antisense SCL suppresses self-renewal and enhances spontaneous erythroid differentiation of the human leukaemic cell line K562.

1991 ◽  
Vol 10 (13) ◽  
pp. 4153-4158 ◽  
Author(s):  
A.R. Green ◽  
E. DeLuca ◽  
C.G. Begley
Keyword(s):  
Cell Line ◽  
Erythroid Differentiation ◽  
Leukaemic Cell ◽  
Self Renewal

The nuclear 3,5,3'-triiodothyronine receptor in human leukaemic cell lines

1984 ◽  
Vol 105 (3) ◽  
pp. 429-432 ◽  
Author(s):  
Juan Bernal ◽  
Leif C. Andersson
Keyword(s):  
Growth Hormone ◽  
Cell Line ◽  
Cell Lines ◽  
Rat Liver ◽  
Association Constant ◽  
Erythroid Differentiation ◽  
Leukaemic Cell ◽  
Cells In Culture ◽  
Gh Cells ◽  
High Concentrations

Abstract. The 3,5,3'-triiodothyronine (T3) receptor has been studied in a series of continuously growing human leukaemic cell lines. High concentrations of receptor were found in the erythroblastoid cell line K-562. T3 was bound to the nuclei of these cells with an association constant of 3.4 × 109 m−1, and capacity 104 fmol/100 μg DNA, or 8700 molecules/nucleus. This capacity is comparable to that of rat liver or growth hormone producing cells (GH cells) in culture, and suggests that the K-562 cell line could be a useful model for the study of T3 action on erythroid differentiation.

Induction of erythroid differentiation in the human leukaemia cell line K562

Nature ◽  
1979 ◽  
Vol 278 (5702) ◽  
pp. 364-365 ◽  
Author(s):  
LEIF C. ANDERSSON ◽  
MIKKO JOKINEN ◽  
CARL G. GAHMBERG
Keyword(s):  
Cell Line ◽  
Erythroid Differentiation ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Human Leukaemia Cell ◽  
Human Leukaemia

scholarly journals Identification of an erythropoietin-sensitive cell line

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1782-1785
Author(s):  
DR Branch ◽  
JM Turc ◽  
LJ Guilbert
Keyword(s):  
Cell Line ◽  
Biochemical Analysis ◽  
Erythroid Differentiation ◽  
Sensitive Cell Line ◽  
Gm Csf ◽  
Dependent Cell ◽  
Macrophage Colony Stimulating Factor ◽  
Survival And Growth ◽  
Macrophage Colony ◽  
Stimulating Factor

The murine lymphoblastic cell line DA-1 has been characterized as dependent upon both interleukin-3 (IL-3, multicolony-stimulating factor [multi-CSF]) and granulocyte-macrophage colony-stimulating factor (GM- CSF) for survival and growth. Here we demonstrate that it is responsive to a third hematopoietic factor, the erythroid-specific hormone, erythropoietin (Epo). DA-1 cells are stimulated to proliferate by partly purified natural murine and human Epo, and pure recombinant human Epo. Antibody to Epo specifically blocks Epo-stimulated growth. Maximal growth stimulated by Epo and GM-CSF is similar, and considerably less than that stimulated by multi-CSF. Proliferation stimulated by Epo and GM-CSF is transient, decreasing within 24 to 48 hours of exposure. However, Epo acts cooperatively with GM-CSF to sustain proliferation. With or without GM-CSF, no obvious erythroid differentiation of DA-1 cells occurs after exposure to Epo for up to 72 hours. This is the first report of a growth factor-dependent cell line also responsive to Epo for survival and growth. The availability of this cell line model should greatly facilitate biochemical analysis of the mechanism of Epo growth-stimulating action.

scholarly journals Expression and modulation of specific, high affinity binding sites for erythropoietin on the human erythroleukemic cell line K562

Blood ◽  
1988 ◽  
Vol 71 (1) ◽  
pp. 104-109 ◽  
Author(s):  
JK Fraser ◽  
FK Lin ◽  
MV Berridge
Keyword(s):  
Cell Line ◽  
K562 Cell ◽  
K562 Cells ◽  
Erythroid Differentiation ◽  
Receptor Expression ◽  
Target Cells ◽  
Epo Receptor ◽  
High Affinity ◽  
Erythroleukemic Cell ◽  
Erythroleukemic Cell Line

Erythroid differentiation is mediated by several interacting factors which include the glycoprotein hormone erythropoietin (Epo), interleukin-3 (IL-3) in the mouse, and erythroid-potentiating activity (EPA) in humans. Each of these factors binds to specific cell surface receptors on responsive target cells, but the way in which these factors interact to modulate erythropoiesis is unknown. In the present study, we used the human erythroleukemic cell line K562 to examine expression and regulation of the receptor for Epo using 125I-labeled, bioactive recombinant human Epo. K562 cells expressed low numbers of a single class of high-affinity Epo receptors corresponding to 4 to 6 receptors per K562 cell (KD = 270 to 290 pmol/L). Treatment of K562 cell cultures with medium conditioned by the EPA-secreting cell line U937 (U937CM) increased receptor expression 2.6 to 3.5-fold to 13 to 17 receptors/cell (KD = 260 to 300 pmol/L). That all of the Epo receptor- potentiating activity in U937CM was accounted for by EPA was shown by a similar increase in Epo receptor expression on K562 cells with recombinant EPA. The effect of U937CM on Epo receptors was reversed by culturing cells in inducer-free medium for 3 days. Medium conditioned by the 5637 cell line had no effect on Epo receptors on K562 cells. In methylcellulose culture, U937CM and Epo acted synergistically to increase erythroid differentiation of K562. Similarly, U937CM stimulated human cord blood CFU-E growth under conditions in which Epo was limiting or in excess. Increases in Epo receptor expression on K562 cells and on CFU-E in response to EPA may mediate the effects of Epo on these cells.

scholarly journals Multi-CSF-dependent colony formation by cells of a murine hemopoietic cell line: specificity and action of multi-CSF

Blood ◽  
1985 ◽  
Vol 65 (2) ◽  
pp. 357-362 ◽  
Author(s):  
D Metcalf
Keyword(s):  
Cell Cycle ◽  
Cell Line ◽  
High Frequency ◽  
Colony Formation ◽  
Hemopoietic Cell ◽  
Self Renewal ◽  
Cell Cycles ◽  
Cell Numbers ◽  
Gm Csf ◽  
Complete Cell

Abstract Cells of the Multi-CSF (IL-3)-dependent hemopoietic cell line 32D c13 formed colonies of varying size in agar cultures stimulated by Multi- CSF. Colony formation was linear with respect to cultured cell numbers; colony numbers and size increased with increasing concentrations of Multi-CSF, and 32D colonies themselves contained a high frequency of clonogenic cells. Clonogenic 32D cells died in the absence of Multi-CSF (half-life six hours), and most were unable to complete cell cycles in progress at the time of withdrawal of Multi-CSF. The concentration of Multi-CSF directly influenced the length of the cell cycle of dividing 32D cells. Purified GM-CSF, G-CSF, or M-CSF had no capacity to support the survival or proliferation of 32D cells. Colonies formed by 32D cells appear to offer a useful model for analyzing the action of Multi- CSF in controlling self-renewal by clonogenic hemopoietic cells.

Sign in / Sign up

Export Citation Format

Share Document