In vitro effect of vanadyl sulfate on cultured primary astrocytes: cell viability and oxidative stress markers.

Severalin vitroandin vivostudies have demonstrated that polyphenol-rich berries may counteract oxidative stress. In this review, we summarized the main finding from human intervention trials on the role of berries in the modulation of markers of oxidative lipid, protein and DNA damage.

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Ceftriaxone Calcium Crystals Induce Acute Kidney Injury by NLRP3-Mediated Inflammation and Oxidative Stress Injury

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/6428498 ◽

2020 ◽

Vol 2020 ◽

pp. 1-13

Author(s):

Zhang Yifan ◽

Ning Benxiang ◽

Xu Zheng ◽

Xu Luwei ◽

Zhou Liuhua ◽

...

Keyword(s):

Oxidative Stress ◽

Kidney Injury ◽

Oxidative Stress Markers ◽

Stress Injury ◽

Stress Markers ◽

Oxidative Stress Injury ◽

Calcium Crystals ◽

And Oxidative Stress

Objective. To investigate the role of inflammatory reactions and oxidative stress injury in the mechanisms of ceftriaxone calcium crystal-induced acute kidney injury (AKI) both in vivo and in vitro. Methods. Male Sprague Dawley rats were randomly divided into five groups of ten each according to different concentrations of ceftriaxone and calcium. Based on the levels of serum creatinine (Scr) and blood urea nitrogen (BUN), the AKI group was chosen for the subsequent experiments. Kidney histological examination and immunohistochemistry were performed. The expression of NLRP3 and IL-1β protein and the concentrations of oxidative stress markers such as ROS, MDA, and H2O2 in kidney tissues were estimated. In parallel, HK-2 human renal proximal tubule cells were exposed to ceftriaxone calcium crystals. The mRNA expression levels of NLRP3 and IL-1β and the concentrations of oxidative stress markers were evaluated. Finally, cell viability and rat survival were also assessed. Results. The results showed that significantly increased Scr and BUN levels, consistent with morphological changes and kidney stones, were found in the rats that received the highest concentration of ceftriaxone (1000 mg/kg) combined with calcium (800 mg/kg). The activation of the NLRP3 inflammasome axis and the marked elevation of MDA, H2O2, and ROS levels were observed both in vivo and in vitro. High expression of Nrf2, HO-1, and NQO1 was also documented. In addition, cell apoptosis and rat mortality were promoted by ceftriaxone calcium crystals. Conclusions. Notably, we found that ceftriaxone-induced urolithiasis was associated with a high risk of AKI and NLRP3-mediated inflammasome and oxidative stress injury were of major importance in the pathogenesis.

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Evaluation of antioxidant status and oxidative stress markers in follicular fluid for human in vitro fertilization outcome

Reproductive Medicine and Biology ◽

10.1002/rmb2.12229 ◽

2018 ◽

Vol 17 (4) ◽

pp. 481-486 ◽

Cited By ~ 6

Author(s):

Takuji Nishihara ◽

Kazuya Matsumoto ◽

Yoshihiko Hosoi ◽

Yoshiharu Morimoto

Keyword(s):

Oxidative Stress ◽

In Vitro Fertilization ◽

Follicular Fluid ◽

Antioxidant Status ◽

Oxidative Stress Markers ◽

Vitro Fertilization ◽

Stress Markers ◽

Human In Vitro Fertilization ◽

And Oxidative Stress

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Leukocytes Count and Oxidative Stress Markers in Blood and Follicular Fluid in Infertile Women Undergo Intracytoplasmic Sperm Injection (Ic

Indian Journal Of Applied Research ◽

10.15373/2249555x/august2014/125 ◽

2011 ◽

Vol 4 (8) ◽

pp. 483-485

Author(s):

Sami R Al-Katib ◽

◽

Basima Sh AlGhazali ◽

Ban J Edan

Keyword(s):

Oxidative Stress ◽

Follicular Fluid ◽

Intracytoplasmic Sperm Injection ◽

Oxidative Stress Markers ◽

Infertile Women ◽

Stress Markers ◽

And Oxidative Stress

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Comparison of Salivary Antioxidants and Oxidative Stress Status in Gestational Diabetes Mellitus and Healthy Pregnant Women

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1568026620666201022151059 ◽

2020 ◽

Vol 20 ◽

Author(s):

Fatemeh Ahmadi-Motamayel ◽

Shima Fathi ◽

Mohammad Taghi Goodarzi ◽

Shiva Borzouei ◽

Jalal Poorolajal ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Gestational Diabetes ◽

Pregnant Women ◽

Oxidative Stress Markers ◽

Blood Samples ◽

Stress Markers ◽

Total Antioxidant ◽

Total Oxidative Stress ◽

And Oxidative Stress

Background: One of the most common complications of pregnant women is gestational diabetes mellitus (GDM). Oxidative stress can play an important role in GDM. Objective: The aim of this study was to evaluate salivary antioxidants and oxidative stress markers in GDM. Method: Twenty pregnant women with GDM and 20 healthy pregnant women with normal blood glucose test participated in this study. Five mL of unstimulated saliva samples were collected. Spectrophotometric assay was carried out for sialochemical analysis. Stata software was used for data analysis. Results: The GDM group exhibited no significant difference in salivary total antioxidant capacity and malondialdehyde compared to the healthy control group. All of antioxidants markers, the uric acid, total antioxidant, peroxidase and catalase, decreased in GDM group that the difference of peroxidase and catalase was statistically significant. All of oxidative stress markers, the salivary malondyaldehid, total oxidative stress and total thiol, increased in GDM group. GDM group exhibited significantly higher salivary total oxidative stress levels. Conclusion: Catalase level was significantly lower and total oxidative stress was significantly higher. These two markers might have significant importance and might exhibit early changes compared to other factors in GDM. . Some of salivary antioxidants might have diagnostic, prognostic or therapeutic implications in GDM. Other studies with large sample size on salivary and blood samples need to be done to confirm this properties and salivary samples using instead of blood samples in GDM biomarkers changes.

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Evaluation of Salivary Antioxidants and Oxidative Stress Markers in Type 2 Diabetes Mellitus: A Retrospective Cohort Study

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666191016103222 ◽

2020 ◽

Vol 20 (4) ◽

pp. 584-590 ◽

Cited By ~ 1

Author(s):

Shima Fathi ◽

Shiva Borzouei ◽

Mohammad Taghi Goodarzi ◽

Jalal Poorolajal ◽

Fatemeh Ahmadi-Motamayel

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Control Group ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Type 2 Dm ◽

Patients With Diabetes ◽

The Difference ◽

And Oxidative Stress

Background: Diabetes Mellitus (DM) is a progressive metabolic disorder. Objective: The aim of this study was to investigate the relationship between antioxidant and oxidative stress markers in the saliva of patients with type 2 DM and a healthy control group. Methods: In this study, 20 patients with diabetes and 20 healthy individuals were evaluated. Salivary antioxidants markers consisted of total antioxidant capacity (TAC), uric acid (UA), peroxidase and catalase. Oxidative stress markers included total oxidant status (TOS), malondealdehyde (MDA) and total thiol (SH). Sialochemical analysis was performed with spectrophotometric assay. All the statistical analyses were conducted using STATA software. Results: TAC decreased significantly in patients with diabetes. Although salivary UA and peroxidase were lower in patients with diabetes compared to the control group, the difference was not significant. Salivary catalase in patients with diabetes was significantly lower than that in the control group. MDA and TOS exhibited significantly higher levels in type 2 DM. SH levels were slightly higher in DM. Conclusions: According to the results of the present study, there were some changes in the salivary levels of some antioxidants and oxidative stress markers in patients with type 2 DM and could be measured as an indicator of serum changes..

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