Toxicological evaluation of Phospholipase D from Kitasatospora paracochleata

2021 ◽  
Author(s):  
Kevin J. Matten ◽  
Alastair Mak
Keyword(s):  
Phospholipase D ◽  
Toxicological Evaluation

Stimulatory effects of phenylephrine and 4β-phorbol-12β 13α-dibutyrate on phospholipase D activity in hypertrophied myocardium in a rat model*

2006 ◽  
Vol 54 (S 1) ◽  
Author(s):  
AA Peivandi ◽  
N Kayhan ◽  
S Salha ◽  
CF Vahl ◽  
K Löffelhoz
Keyword(s):  
Phospholipase D ◽  
Rat Model ◽  
Hypertrophied Myocardium

Toxicological evaluation of xanthan gum based hydrogel formulation in Wistar rats using single dose study

2020 ◽  
Vol 77 (2) ◽  
pp. 353-360
Author(s):  
Nadia Malik ◽  
Mahmood Ahmad ◽  
Muhammad Minhas ◽  
Ruqia Tulain ◽  
Ikrima Khalid ◽  
...  
Keyword(s):  
Single Dose ◽  
Xanthan Gum ◽  
Wistar Rats ◽  
Toxicological Evaluation ◽  
Single Dose Study ◽  
Dose Study ◽  
Hydrogel Formulation

Toxicological evaluation of Aristolochia longa L. extract in mice

2011 ◽  
Vol 4 (5) ◽  
pp. 26-30 ◽  
Author(s):  
Cherif H.S Cherif H.S ◽  
◽  
Saidi F Saidi F ◽  
Guedioura A Guedioura A
Keyword(s):  
Toxicological Evaluation

Using of Phospholipase D of Corynebacterium Pseudotuberculosis Ovis as Immunostimulant with Some Poultry Vaccines

2015 ◽  
Vol 43 (1) ◽  
pp. 174-181
Author(s):  
Marwah M ◽  
Hanan Mahmoud ◽  
Mona El-Shehedi ◽  
Namaa Mohamed
Keyword(s):  
Phospholipase D ◽  
Corynebacterium Pseudotuberculosis

Role of Phospholipase D Inhibitor in Regulating Expression of Senescencerelated Phospholipase D gene in Postharvest Longan Fruit

2019 ◽  
Vol 14 (7) ◽  
pp. 649-657 ◽  
Author(s):  
Li Li ◽  
Jiemin Li ◽  
Jian Sun ◽  
Ping Yi ◽  
Changbao Li ◽  
...  
Keyword(s):  
Phospholipase D ◽  
Ascorbic Acid Content ◽  
Titratable Acidity ◽  
Significant Inhibition ◽  
Solid Content ◽  
Total Soluble Solid ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Soluble Solid ◽  
Pericarp Browning

Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 µL/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 µL/L 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 µL/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.

Biological and Toxicological Evaluation of N-(4methyl-phenyl)-4-methylphthalimide on Bone Cancer in Mice

2019 ◽  
Vol 19 (5) ◽  
pp. 667-676
Author(s):  
José R. Santin ◽  
Gislaine F. da Silva ◽  
Maria V.D. Pastor ◽  
Milena F. Broering ◽  
Roberta Nunes ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Metastasis ◽  
In Silico ◽  
Bone Matrix ◽  
Micronucleus Assay ◽  
Repeated Treatment ◽  
Toxicological Evaluation ◽  
Toxicological Analysis ◽  
Breast Cancer Bone Metastasis

Background: It was recently demonstrated that the phthalimide N-(4-methyl-phenyl)-4- methylphthalimide (MPMPH-1) has important effects against acute and chronic pain in mice, with a mechanism of action correlated to adenylyl cyclase inhibition. Furthermore, it was also demonstrated that phthalimide derivatives presented antiproliferative and anti-tumor effects. Considering the literature data, the present study evaluated the effects of MPMPH-1 on breast cancer bone metastasis and correlated painful symptom, and provided additional toxicological information about the compound and its possible metabolites. Methods: In silico toxicological analysis was supported by in vitro and in vivo experiments to demonstrate the anti-tumor and anti-hypersensitivity effects of the compound. Results: The data obtained with the in silico toxicological analysis demonstrated that MPMPH-1 has mutagenic potential, with a low to moderate level of confidence. The mutagenicity potential was in vivo confirmed by micronucleus assay. MPMPH-1 treatments in the breast cancer bone metastasis model were able to prevent the osteoclastic resorption of bone matrix. Regarding cartilage, degradation was considerably reduced within the zoledronic acid group, while in MPMPH-1, chondrocyte multiplication was observed in random areas, suggesting bone regeneration. Additionally, the repeated treatment of mice with MPMPH-1 (10 mg/kg, i.p.), once a day for up to 36 days, significantly reduces the hypersensitivity in animals with breast cancer bone metastasis. Conclusion: Together, the data herein obtained show that MPMPH-1 is relatively safe, and significantly control the cancer growth, allied to the reduction in bone reabsorption and stimulation of bone and cartilage regeneration. MPMPH-1 effects may be linked, at least in part, to the ability of the compound to interfere with adenylylcyclase pathway activation.

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