Improved performance of the SH test as an in vitro skin sensitization test with a new predictive model and decision tree

This work presents the development of multifunctional therapeutic membranes based on a high-performance block copolymer scaffold formed by polyether (PE) and polyamide (PA) units (known as PEBA) and layered double hydroxide (LDH) biomaterials, with the aim to study their uses as wound dressings. Two LDH layer compositions were employed containing Mg2+ or Zn2+, Fe3+ and Al3+ cations, intercalated with chloride anions, abbreviated as Mg-Cl or Zn-Cl, or intercalated with naproxenate (NAP) anions, abbreviated as Mg-NAP or Zn-NAP. Membranes were structurally and physically characterized, and the in vitro drug release kinetics and cytotoxicity assessed. PEBA-loading NaNAP salt particles were also prepared for comparison. Intercalated NAP anions improved LDH–polymer interaction, resulting in membranes with greater mechanical performance compared to the polymer only or to the membranes containing the Cl-LDHs. Drug release (in saline solution) was sustained for at least 8 h for all samples and release kinetics could be modulated: a slower, an intermediate and a faster NAP release were observed from membranes containing Zn-NAP, NaNAP and Mg-NAP particles, respectively. In general, cell viability was higher in the presence of Mg-LDH and the membranes presented improved performance in comparison with the powdered samples. PEBA containing Mg-NAP sample stood out among all membranes in all the evaluated aspects, thus being considered a great candidate for application as multifunctional therapeutic dressings.

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In Vitro and In Vivo Biosafety Analysis of Resorbable Polyglycolic Acid-Polylactic Acid Block Copolymer Composites for Spinal Fixation

Polymers ◽

10.3390/polym13010029 ◽

2020 ◽

Vol 13 (1) ◽

pp. 29

Author(s):

Seung Kyun Yoon ◽

Jin Ho Yang ◽

Hyun Tae Lim ◽

Young-Wook Chang ◽

Muhammad Ayyoob ◽

...

Keyword(s):

Lactic Acid ◽

Animal Experiments ◽

Polymeric Materials ◽

Polyglycolic Acid ◽

Poly Lactic Acid ◽

Skin Sensitization ◽

Spinal Fixation ◽

In Vivo Degradation

Herein, spinal fixation implants were constructed using degradable polymeric materials such as PGA–PLA block copolymers (poly(glycolic acid-b-lactic acid)). These materials were reinforced by blending with HA-g-PLA (hydroxyapatite-graft-poly lactic acid) and PGA fiber before being tested to confirm its biocompatibility via in vitro (MTT assay) and in vivo animal experiments (i.e., skin sensitization, intradermal intracutaneous reaction, and in vivo degradation tests). Every specimen exhibited suitable biocompatibility and biodegradability for use as resorbable spinal fixation materials.

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Molecular and biophysical mechanisms behind the enhancement of lung surfactant function during controlled therapeutic hypothermia

Scientific Reports ◽

10.1038/s41598-020-79025-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

C. Autilio ◽

M. Echaide ◽

A. Cruz ◽

C. Mouton ◽

A. Hidalgo ◽

...

Keyword(s):

Therapeutic Hypothermia ◽

Molecular Mechanisms ◽

Lung Surfactant ◽

Interfacial Structure ◽

Surfactant Activity ◽

Improved Performance ◽

Biophysical Activity ◽

Respiratory Outcomes

AbstractTherapeutic hypothermia (TH) enhances pulmonary surfactant performance in vivo by molecular mechanisms still unknown. Here, the interfacial structure and the composition of lung surfactant films have been analysed in vitro under TH as well as the molecular basis of its improved performance both under physiological and inhibitory conditions. The biophysical activity of a purified porcine surfactant was tested under slow and breathing-like dynamics by constrained drop surfactometry (CDS) and in the captive bubble surfactometer (CBS) at both 33 and 37 °C. Additionally, the temperature-dependent surfactant activity was also analysed upon inhibition by plasma and subsequent restoration by further surfactant supplementation. Interfacial performance was correlated with lateral structure and lipid composition of films made of native surfactant. Lipid/protein mixtures designed as models to mimic different surfactant contexts were also studied. The capability of surfactant to drastically reduce surface tension was enhanced at 33 °C. Larger DPPC-enriched domains and lower percentages of less active lipids were detected in surfactant films exposed to TH-like conditions. Surfactant resistance to plasma inhibition was boosted and restoration therapies were more effective at 33 °C. This may explain the improved respiratory outcomes observed in cooled patients with acute respiratory distress syndrome and opens new opportunities in the treatment of acute lung injury.

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Evaluation of the Skin Sensitization Potential of Silica nanoparticles using in vitro and in vivo assay

Toxicology Letters ◽

10.1016/s0378-4274(21)00415-x ◽

2021 ◽

Vol 350 ◽

pp. S72

Author(s):

D.H. Lee ◽

S.-H. Kim ◽

J.H. Lee ◽

J.-Y Yang ◽

H.-S. Shin ◽

...

Keyword(s):

Silica Nanoparticles ◽

Skin Sensitization ◽

In Vivo Assay

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The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test – human cell line activation test (h-CLAT)

Cell Biology and Toxicology ◽

10.1007/s10565-008-9059-9 ◽

2008 ◽

Vol 25 (2) ◽

pp. 109-126 ◽

Cited By ~ 62

Author(s):

Hitoshi Sakaguchi ◽

Takao Ashikaga ◽

Masaaki Miyazawa ◽

Nanae Kosaka ◽

Yuichi Ito ◽

...

Keyword(s):

Cell Viability ◽

Cell Line ◽

Human Cell ◽

Human Cell Line ◽

Skin Sensitization ◽

Activation Test ◽

The Relationship

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Development of an in vitro skin sensitization test based on ROS production in THP-1 cells

Toxicology in Vitro ◽

10.1016/j.tiv.2012.12.025 ◽

2013 ◽

Vol 27 (2) ◽

pp. 857-863 ◽

Cited By ~ 17

Author(s):

Kazutoshi Saito ◽

Masaaki Miyazawa ◽

Yuko Nukada ◽

Hitoshi Sakaguchi ◽

Naohiro Nishiyama

Keyword(s):

Ros Production ◽

Skin Sensitization

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Improved techniques for liquid culture of human and mouse bone marrow

Blood ◽

10.1182/blood.v47.3.369.bloodjournal473369 ◽

1976 ◽

Vol 47 (3) ◽

pp. 369-379

Author(s):

MJ Cline ◽

DW Golde

Keyword(s):

Bone Marrow ◽

Stem Cell ◽

Large Scale ◽

Membrane Surface ◽

Diffusion Chamber ◽

Mononuclear Phagocytes ◽

Mouse Bone Marrow ◽

Improved Performance ◽

Proliferation In Vitro

Previous studies using the in vitro diffusion chamber (Marbrook) have shown that bone marrow grown in this system will undergo limited stem cell replication and differentiation to mature granulocytes and mononuclear phagocytes. A series of studies with modified culture systems was initiated to improve cell production and committed stem cell (CFU-C) proliferation in vitro. Introduction of a continuous-flow system and a migration technique providing means of egress for mature neutrophils resulted in substantially improved performance. CFU-C were found to be capable of migration through a 3-mu pore membrane. These studies indicated that membrane surface area, culture medium circulation, and mature cell egress were among the conditions that could be optimized for maximum hematopoietic cell proliferation in suspension culture. The present observations also suggested that large- scale in vitro growth of mammalian bone marrow may be feasible.

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